C1DI48 (RNPH_AZOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Avin_02850

Organism

Azotobacter vinelandii (strain DJ / ATCC BAA-1303) [Complete proteome] [HAMAP]

Taxonomic identifier

322710 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter › Azotobacter vinelandii

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease PH HAMAP-Rule MF_00564		PRO_1000212059

Sequences

	Sequence		Length	Mass (Da)
	C1DI48 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 147199A7E03F8485	FASTA	239	25,826
	10 20 30 40 50 60 MKRPSGRAPH QLRPIRITRN YTRHAEGSVL VEFGDTRVIC TVSAEAGVPR FLKGQGKGWL 70 80 90 100 110 120 TAEYGMLPRA TGERMAREAS RGRQGGRTLE IQRLIGRSLR AALDMSKLGE NTLYVDCDVI 130 140 150 160 170 180 QADGGTRTAS ITGAMVALVD ALAVLKKRGA LKGDPLKQMV AAVSVGIYQG EPILDLDYPE 190 200 210 220 230 DSDAETDLNV VMTDAGGFIE VQGTAEGEPF QPEELNAMLA LATQGIRELF ELQRAVLAD

References

[1]

"Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes." Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G., Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L., Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A., Houmiel K., Imperial J. , Kennedy C., Larson T.J., Latreille P., Ligon L.S., Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I., Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L., Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H., Dean D.R., Dixon R., Wood D. J. Bacteriol. 191:4534-4545(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJ / ATCC BAA-1303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001157 Genomic DNA. Translation: ACO76545.1.
RefSeq	YP_002797520.1. NC_012560.1.
3D structure databases
ProteinModelPortal	C1DI48.
SMR	C1DI48. Positions 1-239.
ModBase	Search...
Protein-protein interaction databases
STRING	322710.Avin_02850.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO76545; ACO76545; Avin_02850.
GeneID	7759245.
KEGG	avn:Avin_02850.
PATRIC	21026924. VBIAzoVin72790_0264.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	AVIN322710:GJ0M-282-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_AZOVD

Accession

Primary (citable) accession number: C1DI48

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program