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C1DFK2

- C1DFK2_AZOVD

UniProt

C1DFK2 - C1DFK2_AZOVD

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei308 – 3081Coenzyme AUniRule annotation
    Binding sitei332 – 3321Coenzyme AUniRule annotation
    Binding sitei384 – 3841Substrate; via nitrogen amideUniRule annotation
    Binding sitei497 – 4971SubstrateUniRule annotation
    Binding sitei512 – 5121SubstrateUniRule annotation
    Active sitei514 – 5141UniRule annotation
    Binding sitei520 – 5201Coenzyme AUniRule annotation
    Binding sitei523 – 5231SubstrateUniRule annotation
    Metal bindingi534 – 5341Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi536 – 5361Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi539 – 5391Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei581 – 5811Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAVIN322710:GJ0M-4277-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Synonyms:acsA2Imported
    Ordered Locus Names:Avin_42750Imported
    OrganismiAzotobacter vinelandii (strain DJ / ATCC BAA-1303)Imported
    Taxonomic identifieri322710 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter
    ProteomesiUP000002424: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei606 – 6061N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi322710.Avin_42750.

    Structurei

    3D structure databases

    ProteinModelPortaliC1DFK2.
    SMRiC1DFK2. Positions 7-642.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni408 – 4136Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiKSLENPE.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C1DFK2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKIANHPVS EALRQHSWLN NDDYLRLYRQ SVEQPDSFWA EQATLFLDWF    50
    KPWRNIHRGD MNKGEIHWFE GSQLNVAHNC IDRHLKQRGD QVAIIWEGDN 100
    PADSARITYR QLHEQVCRLA NLLKSRGIGK GDRVCIYMPM IPEAAYAMLA 150
    CARIGAVHSV VFGGFSPEAL RDRILDADCR LLITADESVR GGKVVPLKRN 200
    ADKALQSCSG VSSVIVVRRT GGAIDWNVGR DLWYHEAVPG CSADCPAEPM 250
    DAEAPLFILY TSGSTGKPKG VLHTTGGYLL QAAMTHQYVF DYHDGDIYWC 300
    SADIGWITGH SYIVYGPLAN GATTLIFEGV PNYPDASRFW QVVDKHRVNI 350
    FYTAPTALRS LMREGEGPVR KTSRASLRLL GSVGEPINPE AWEWYYHVVG 400
    DSRCPIVDTW WQTETGAIMI TPLPGATLLK PGSATRPFFG VQPVLLDDKG 450
    KEIEGPGAGI LAIKASWPSQ IRSIYGDHQR LIDTYFKPCP GYYFTGDGAR 500
    RDEDGYYWIT GRVDDVINVS GHRIGTAEVE SALVLHDSIA EAAVVGCPHD 550
    VKGQCVYAFV TLVTGSRPSD QLKRELIDQV SREIGGFAKP DFLQWAPRLP 600
    KTRSGKIMRR ILRKIACNEL DNLGDTSTLS DPSVLQELID NRLSH 645
    Length:645
    Mass (Da):71,897
    Last modified:May 26, 2009 - v1
    Checksum:i2196F80A1AE5C342
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001157 Genomic DNA. Translation: ACO80398.1.
    RefSeqiYP_002801373.1. NC_012560.1.

    Genome annotation databases

    EnsemblBacteriaiACO80398; ACO80398; Avin_42750.
    GeneIDi7763150.
    KEGGiavn:Avin_42750.
    PATRICi21034580. VBIAzoVin72790_4023.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001157 Genomic DNA. Translation: ACO80398.1 .
    RefSeqi YP_002801373.1. NC_012560.1.

    3D structure databases

    ProteinModelPortali C1DFK2.
    SMRi C1DFK2. Positions 7-642.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 322710.Avin_42750.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACO80398 ; ACO80398 ; Avin_42750 .
    GeneIDi 7763150.
    KEGGi avn:Avin_42750.
    PATRICi 21034580. VBIAzoVin72790_4023.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi KSLENPE.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci AVIN322710:GJ0M-4277-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DJ / ATCC BAA-1303Imported.

    Entry informationi

    Entry nameiC1DFK2_AZOVD
    AccessioniPrimary (citable) accession number: C1DFK2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 26, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3