C1DDA6 (SYT_LARHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	LHK_02757

Organism

Laribacter hongkongensis (strain HLHK9) [Complete proteome] [HAMAP]

Taxonomic identifier

557598 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Laribacter

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000199554
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1DDA6 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: C23244A849CF43FD	FASTA	633	71,580
	10 20 30 40 50 60 MPAIRLPDGS VRQFENPVSV HDVAASIGAG LARAALAGRV DGALVDTSFV IDHDVDLAIV 70 80 90 100 110 120 TDKDADGLGI LRHSTAHLLA CAVKELFPEA QVTIGPEIEN GFYYDFSYKR PFTPEDLVAI 130 140 150 160 170 180 EKKMAELAKK DIPVERYELP RDEAIAYFKS IGEAYKAEII ESIPQGEVLS LYREGNFTDL 190 200 210 220 230 240 CRGPHVPSTG KLKVFKLMKV AGAYWRGDSK NEMLQRIYGT AWAKKEDLEA YLHMLEEAEK 250 260 270 280 290 300 RDHRKLGRQL DLFHIQDEAP GMVFWHPKGW TLWQQVEQYM RRVLIANDYQ EVRTPQIVDR 310 320 330 340 350 360 SLWEKSGHWA MYQDLMFTTE SEKRDYAVKP MNCPCHIQIF NQGLKSYRDL PLRMAEFGSC 370 380 390 400 410 420 HRNEPSGSLH GIMRVRNFVQ DDAHIFCTEA QMQPETAAFI DLLQAVYQDF GFDNILIKLS 430 440 450 460 470 480 TRPEKRVGAD ELWDQAEAAL AAALAAKGLA YELQPGEGAF YGPKIEFSLK DCLGRVWQCG 490 500 510 520 530 540 TLQLDFNLPV RLGAEYVSED NAKKHPVMLH RAILGSLERF IGILIEHHAG AMPLWLAPVQ 550 560 570 580 590 600 MAVLNITEAQ AEYATEVAET LKKQGFRVDL DLRNEKISYK IREHSLQKLP YQIIVGDKEK 610 620 630 AGALVAVRAR TGEDLGQIPL SQFIERLKSE LLH

References

[1]

"The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats." Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y. PLoS Genet. 5:E1000416-E1000416(2009) [PubMed: 19283063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HLHK9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001154 Genomic DNA. Translation: ACO75738.1.
RefSeq	YP_002796747.1. NC_012559.1.
3D structure databases
ProteinModelPortal	C1DDA6.
ModBase	Search...
Protein-protein interaction databases
STRING	C1DDA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7758367.
GenomeReviews	Gene locus LHK_02757 in contig CP001154_GR.
KEGG	lhk:LHK_02757.
PATRIC	22302284. VBILarHon49832_2512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VGSDALW.
ProtClustDB	PRK00413.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_LARHH

Accession

Primary (citable) accession number: C1DDA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program