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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594Aminoacyl-adenylateUniRule annotation1
Binding sitei638ATPUniRule annotation1
Metal bindingi920ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1
Metal bindingi940ZincUniRule annotation1
Metal bindingi943ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLHON557598:G1GCR-591-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LHK_00614
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
Proteomesi
  • UP000002010 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162391 – 957Isoleucine--tRNA ligaseAdd BLAST957

Proteomic databases

PRIDEiC1DCX2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557598.LHK_00614

Structurei

3D structure databases

ProteinModelPortaliC1DCX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi635 – 639"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C1DCX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLKKTVNLL DTPFPMRGDL ARREPGWLAQ WQAQQRYQKL RKIAAGRPKF
60 70 80 90 100
ILHDGPPYAN GDIHIGHAVN KILKDIIVRS KTQAGFDAPY VPGWDCHGLP
110 120 130 140 150
IEHQIEKLVK GDKKAIEAAP SIHARITEYR KANGLDAKAT DLPASFIREL
160 170 180 190 200
CREYAGLQVE RQKKDFIRLG VLGEWDNPYR TMSFRSEADE IRALGKLYQQ
210 220 230 240 250
GYLFKGLKPV YWCFDCGSAL AEAEVEYQDK TSPTIDVAFA SAEPEKLAAA
260 270 280 290 300
FGLSALPAGK TASAVIWTTT PWTIPANQAL NVHPDFDYGL YDTEKGLLIL
310 320 330 340 350
AADLAKGALE RFGLTGVEVA RCKGQALDRL TFRHPFYDRV SPVYVGDYVT
360 370 380 390 400
LDAGTGIVHS APAYGLEDFD SCRKNGMPND AILNPVTSDG TYVDSLPFFG
410 420 430 440 450
GLHIWKANPL IVEKLAEVGA LMHTSPLSHS YPHCWRHKTP VVYRATTQWF
460 470 480 490 500
VGMDREVAGS TLRQRALKGV DDTAFFPAWG QARLHAMIAN RPDWCVSRQR
510 520 530 540 550
NWGVPIPFFL DKQTGEPHPR TPELLEAVAR KVEEQGINAW FELDPAELLG
560 570 580 590 600
EDAARFDKMK DTLDVWFDSG TTHFHVLRGT HAEQLAYPAD LYLEGSDQHR
610 620 630 640 650
GWFHSSLLTG CALDGRAPYN QLLTHGFVVD GQGRKMSKSV GNVIAPQKIN
660 670 680 690 700
DSLGADILRL WVASTDYSGE LAISDTILKG TTDSYRRLRN TIRFLLANLS
710 720 730 740 750
DFNPATDALP VSELTELDRY ALVLAQRLHA GVAEDCYPRY AFHTAMQAIV
760 770 780 790 800
GYCTDDLGAF WLDIIKDRLY TTKADSKARR SAQTALWHVT RSLLSLLAPV
810 820 830 840 850
LCFTADEAWQ ALTGEAEDSP VYHTWHELPV VADAEALAAR WDVLRALRAQ
860 870 880 890 900
INKDIETLRE AGAVGSSLQA EVDIEADAGL YPLLNALGDE LKFVLIVSRV
910 920 930 940 950
GVVPGPETRI RVSASGEQKC ERCWHYHPTV GENAEAPTLC ARCYDNIFGQ

GESRSYA
Length:957
Mass (Da):106,364
Last modified:May 26, 2009 - v1
Checksum:iF4891ED2EA3BB86F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA Translation: ACO73607.1
RefSeqiWP_012696099.1, NC_012559.1

Genome annotation databases

EnsemblBacteriaiACO73607; ACO73607; LHK_00614
KEGGilhk:LHK_00614

Similar proteinsi

Entry informationi

Entry nameiSYI_LARHH
AccessioniPrimary (citable) accession number: C1DCX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: March 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health