Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C1DCX2

- SYI_LARHH

UniProt

C1DCX2 - SYI_LARHH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941Aminoacyl-adenylateUniRule annotation
Binding sitei638 – 6381ATPUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi940 – 9401ZincUniRule annotation
Metal bindingi943 – 9431ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLHON557598:GHO5-622-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LHK_00614
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
ProteomesiUP000002010: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 957957Isoleucine--tRNA ligasePRO_1000216239Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557598.LHK_00614.

Structurei

3D structure databases

ProteinModelPortaliC1DCX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi635 – 6395"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1DCX2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLKKTVNLL DTPFPMRGDL ARREPGWLAQ WQAQQRYQKL RKIAAGRPKF
60 70 80 90 100
ILHDGPPYAN GDIHIGHAVN KILKDIIVRS KTQAGFDAPY VPGWDCHGLP
110 120 130 140 150
IEHQIEKLVK GDKKAIEAAP SIHARITEYR KANGLDAKAT DLPASFIREL
160 170 180 190 200
CREYAGLQVE RQKKDFIRLG VLGEWDNPYR TMSFRSEADE IRALGKLYQQ
210 220 230 240 250
GYLFKGLKPV YWCFDCGSAL AEAEVEYQDK TSPTIDVAFA SAEPEKLAAA
260 270 280 290 300
FGLSALPAGK TASAVIWTTT PWTIPANQAL NVHPDFDYGL YDTEKGLLIL
310 320 330 340 350
AADLAKGALE RFGLTGVEVA RCKGQALDRL TFRHPFYDRV SPVYVGDYVT
360 370 380 390 400
LDAGTGIVHS APAYGLEDFD SCRKNGMPND AILNPVTSDG TYVDSLPFFG
410 420 430 440 450
GLHIWKANPL IVEKLAEVGA LMHTSPLSHS YPHCWRHKTP VVYRATTQWF
460 470 480 490 500
VGMDREVAGS TLRQRALKGV DDTAFFPAWG QARLHAMIAN RPDWCVSRQR
510 520 530 540 550
NWGVPIPFFL DKQTGEPHPR TPELLEAVAR KVEEQGINAW FELDPAELLG
560 570 580 590 600
EDAARFDKMK DTLDVWFDSG TTHFHVLRGT HAEQLAYPAD LYLEGSDQHR
610 620 630 640 650
GWFHSSLLTG CALDGRAPYN QLLTHGFVVD GQGRKMSKSV GNVIAPQKIN
660 670 680 690 700
DSLGADILRL WVASTDYSGE LAISDTILKG TTDSYRRLRN TIRFLLANLS
710 720 730 740 750
DFNPATDALP VSELTELDRY ALVLAQRLHA GVAEDCYPRY AFHTAMQAIV
760 770 780 790 800
GYCTDDLGAF WLDIIKDRLY TTKADSKARR SAQTALWHVT RSLLSLLAPV
810 820 830 840 850
LCFTADEAWQ ALTGEAEDSP VYHTWHELPV VADAEALAAR WDVLRALRAQ
860 870 880 890 900
INKDIETLRE AGAVGSSLQA EVDIEADAGL YPLLNALGDE LKFVLIVSRV
910 920 930 940 950
GVVPGPETRI RVSASGEQKC ERCWHYHPTV GENAEAPTLC ARCYDNIFGQ

GESRSYA
Length:957
Mass (Da):106,364
Last modified:May 26, 2009 - v1
Checksum:iF4891ED2EA3BB86F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA. Translation: ACO73607.1.
RefSeqiWP_012696099.1. NC_012559.1.
YP_002794616.1. NC_012559.1.

Genome annotation databases

EnsemblBacteriaiACO73607; ACO73607; LHK_00614.
GeneIDi7756237.
KEGGilhk:LHK_00614.
PATRICi22298325. VBILarHon49832_0585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA. Translation: ACO73607.1 .
RefSeqi WP_012696099.1. NC_012559.1.
YP_002794616.1. NC_012559.1.

3D structure databases

ProteinModelPortali C1DCX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557598.LHK_00614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO73607 ; ACO73607 ; LHK_00614 .
GeneIDi 7756237.
KEGGi lhk:LHK_00614.
PATRICi 22298325. VBILarHon49832_0585.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LHON557598:GHO5-622-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats."
    Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y.
    PLoS Genet. 5:E1000416-E1000416(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLHK9.

Entry informationi

Entry nameiSYI_LARHH
AccessioniPrimary (citable) accession number: C1DCX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3