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C1DCX2

- SYI_LARHH

UniProt

C1DCX2 - SYI_LARHH

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LHK_00614
Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941Aminoacyl-adenylate By similarity
Binding sitei638 – 6381ATP By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi940 – 9401Zinc By similarity
Metal bindingi943 – 9431Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLHON557598:GHO5-622-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LHK_00614
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
ProteomesiUP000002010: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 957957Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216239Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi557598.LHK_00614.

Structurei

3D structure databases

ProteinModelPortaliC1DCX2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi635 – 6395"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1DCX2-1 [UniParc]FASTAAdd to Basket

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MDLKKTVNLL DTPFPMRGDL ARREPGWLAQ WQAQQRYQKL RKIAAGRPKF    50
ILHDGPPYAN GDIHIGHAVN KILKDIIVRS KTQAGFDAPY VPGWDCHGLP 100
IEHQIEKLVK GDKKAIEAAP SIHARITEYR KANGLDAKAT DLPASFIREL 150
CREYAGLQVE RQKKDFIRLG VLGEWDNPYR TMSFRSEADE IRALGKLYQQ 200
GYLFKGLKPV YWCFDCGSAL AEAEVEYQDK TSPTIDVAFA SAEPEKLAAA 250
FGLSALPAGK TASAVIWTTT PWTIPANQAL NVHPDFDYGL YDTEKGLLIL 300
AADLAKGALE RFGLTGVEVA RCKGQALDRL TFRHPFYDRV SPVYVGDYVT 350
LDAGTGIVHS APAYGLEDFD SCRKNGMPND AILNPVTSDG TYVDSLPFFG 400
GLHIWKANPL IVEKLAEVGA LMHTSPLSHS YPHCWRHKTP VVYRATTQWF 450
VGMDREVAGS TLRQRALKGV DDTAFFPAWG QARLHAMIAN RPDWCVSRQR 500
NWGVPIPFFL DKQTGEPHPR TPELLEAVAR KVEEQGINAW FELDPAELLG 550
EDAARFDKMK DTLDVWFDSG TTHFHVLRGT HAEQLAYPAD LYLEGSDQHR 600
GWFHSSLLTG CALDGRAPYN QLLTHGFVVD GQGRKMSKSV GNVIAPQKIN 650
DSLGADILRL WVASTDYSGE LAISDTILKG TTDSYRRLRN TIRFLLANLS 700
DFNPATDALP VSELTELDRY ALVLAQRLHA GVAEDCYPRY AFHTAMQAIV 750
GYCTDDLGAF WLDIIKDRLY TTKADSKARR SAQTALWHVT RSLLSLLAPV 800
LCFTADEAWQ ALTGEAEDSP VYHTWHELPV VADAEALAAR WDVLRALRAQ 850
INKDIETLRE AGAVGSSLQA EVDIEADAGL YPLLNALGDE LKFVLIVSRV 900
GVVPGPETRI RVSASGEQKC ERCWHYHPTV GENAEAPTLC ARCYDNIFGQ 950
GESRSYA 957
Length:957
Mass (Da):106,364
Last modified:May 26, 2009 - v1
Checksum:iF4891ED2EA3BB86F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001154 Genomic DNA. Translation: ACO73607.1.
RefSeqiWP_012696099.1. NC_012559.1.
YP_002794616.1. NC_012559.1.

Genome annotation databases

EnsemblBacteriaiACO73607; ACO73607; LHK_00614.
GeneIDi7756237.
KEGGilhk:LHK_00614.
PATRICi22298325. VBILarHon49832_0585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001154 Genomic DNA. Translation: ACO73607.1 .
RefSeqi WP_012696099.1. NC_012559.1.
YP_002794616.1. NC_012559.1.

3D structure databases

ProteinModelPortali C1DCX2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 557598.LHK_00614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO73607 ; ACO73607 ; LHK_00614 .
GeneIDi 7756237.
KEGGi lhk:LHK_00614.
PATRICi 22298325. VBILarHon49832_0585.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LHON557598:GHO5-622-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats."
    Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y.
    PLoS Genet. 5:E1000416-E1000416(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLHK9.

Entry informationi

Entry nameiSYI_LARHH
AccessioniPrimary (citable) accession number: C1DCX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi