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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi56Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi60Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi88Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei90Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei157Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei182Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei186Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei379Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei383Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei489Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei538Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei565Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei805Substrate; via amide nitrogenUniRule annotation1
Binding sitei813Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei830Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciLHON557598:G1GCR-1924-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:LHK_02081
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
Proteomesi
  • UP000002010 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Tat-type signalUniRule annotationAdd BLAST34
ChainiPRO_100018636635 – 839Periplasmic nitrate reductaseUniRule annotationAdd BLAST805

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi557598.LHK_02081

Structurei

3D structure databases

ProteinModelPortaliC1D9G3
SMRiC1D9G3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 1024Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 226Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni250 – 254Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni269 – 271Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni515 – 516Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni729 – 738Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C1D9G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLTRRDFIK ANAAAAAATA AAVNLPLVPS MAQAATTDPA TAGADIKWDK
60 70 80 90 100
AACRFCGTGC SVLVGTKGGR VVATQGDPDA PVNRGLNCIK GYFLSKIMYG
110 120 130 140 150
EDRLTKPLLR MKNGKFDKNG EFTPVTWKQA FDVMEEKFKT AMKAGGPEAV
160 170 180 190 200
AMFGSGQWTI WEGYAAAKFM KAGLRSNNLD PNARHCMASA VVGFMRTFNM
210 220 230 240 250
DEPMGCYDDI EKADAFVLWG SNMAEMHPIL WSRITDRRLT HPDCQVHVLS
260 270 280 290 300
TYEHRSFELA DNKLVFGPQT DLAILNYIAN HIIQTGAVNK DFIQKYCAFM
310 320 330 340 350
KGDTDIGYGL RPTHPKQKAA KNPDSGKMDP ITFEQFAAFV KPYTLEYTAR
360 370 380 390 400
TSCVPAERLK RLAELYADPK KKVVSFWTMG FNQHTRGTWA NNLVYNIHLL
410 420 430 440 450
TGKISTPGCG PFSLTGQPSA CGTAREVGTF AHRLPADLVV MNPEHRKIAE
460 470 480 490 500
NIWKLPEGTI PPKMGLHAVA MQRALKDSKL KVYWQQCNNN MQAGPNINEE
510 520 530 540 550
MYPGWRNPET FIIVSDPYPT VSAMAADLIL PTAMWVEKEG AYGNAERRTQ
560 570 580 590 600
FWRQQVNAPG EAKSDLWQVV EFSKRFKVEE VWPAELVAKK PEYRGKTLYD
610 620 630 640 650
VLFANGQVNK YKLDDMARQH GTAYHNEEAR HVGFYLQKGL FEEYAAFGRG
660 670 680 690 700
HGHDLALFDT YHKARGLRWP VVDGKETLWR YREGFDPYVK KGEGVRFYGQ
710 720 730 740 750
KDGRARIIAL PFEPAAEVPD REYNMWLCTG RVLEHWHTGS MTRRVPELYR
760 770 780 790 800
AVPDAQVFMH PADAELRGLK RGQQVKVISR RGEITLTLET RGRNKPPQGL
810 820 830
VFIPFFDEGR LVNKLTLDAT CPLSKETDYK KCAVKVVRA
Length:839
Mass (Da):94,304
Last modified:May 26, 2009 - v1
Checksum:i1C02424A6F70B510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA Translation: ACO75065.1
RefSeqiWP_012697551.1, NC_012559.1

Genome annotation databases

EnsemblBacteriaiACO75065; ACO75065; LHK_02081
KEGGilhk:LHK_02081

Similar proteinsi

Entry informationi

Entry nameiNAPA_LARHH
AccessioniPrimary (citable) accession number: C1D9G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: March 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health