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C1D9G3

- NAPA_LARHH

UniProt

C1D9G3 - NAPA_LARHH

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi56 – 561Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi88 – 881Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciLHON557598:GHO5-2111-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:LHK_02081
    OrganismiLaribacter hongkongensis (strain HLHK9)
    Taxonomic identifieri557598 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
    ProteomesiUP000002010: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Tat-type signalUniRule annotationAdd
    BLAST
    Chaini35 – 839805Periplasmic nitrate reductasePRO_1000186366Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi557598.LHK_02081.

    Structurei

    3D structure databases

    ProteinModelPortaliC1D9G3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 102574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiCACKVER.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C1D9G3-1 [UniParc]FASTAAdd to Basket

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    MTLTRRDFIK ANAAAAAATA AAVNLPLVPS MAQAATTDPA TAGADIKWDK    50
    AACRFCGTGC SVLVGTKGGR VVATQGDPDA PVNRGLNCIK GYFLSKIMYG 100
    EDRLTKPLLR MKNGKFDKNG EFTPVTWKQA FDVMEEKFKT AMKAGGPEAV 150
    AMFGSGQWTI WEGYAAAKFM KAGLRSNNLD PNARHCMASA VVGFMRTFNM 200
    DEPMGCYDDI EKADAFVLWG SNMAEMHPIL WSRITDRRLT HPDCQVHVLS 250
    TYEHRSFELA DNKLVFGPQT DLAILNYIAN HIIQTGAVNK DFIQKYCAFM 300
    KGDTDIGYGL RPTHPKQKAA KNPDSGKMDP ITFEQFAAFV KPYTLEYTAR 350
    TSCVPAERLK RLAELYADPK KKVVSFWTMG FNQHTRGTWA NNLVYNIHLL 400
    TGKISTPGCG PFSLTGQPSA CGTAREVGTF AHRLPADLVV MNPEHRKIAE 450
    NIWKLPEGTI PPKMGLHAVA MQRALKDSKL KVYWQQCNNN MQAGPNINEE 500
    MYPGWRNPET FIIVSDPYPT VSAMAADLIL PTAMWVEKEG AYGNAERRTQ 550
    FWRQQVNAPG EAKSDLWQVV EFSKRFKVEE VWPAELVAKK PEYRGKTLYD 600
    VLFANGQVNK YKLDDMARQH GTAYHNEEAR HVGFYLQKGL FEEYAAFGRG 650
    HGHDLALFDT YHKARGLRWP VVDGKETLWR YREGFDPYVK KGEGVRFYGQ 700
    KDGRARIIAL PFEPAAEVPD REYNMWLCTG RVLEHWHTGS MTRRVPELYR 750
    AVPDAQVFMH PADAELRGLK RGQQVKVISR RGEITLTLET RGRNKPPQGL 800
    VFIPFFDEGR LVNKLTLDAT CPLSKETDYK KCAVKVVRA 839
    Length:839
    Mass (Da):94,304
    Last modified:May 26, 2009 - v1
    Checksum:i1C02424A6F70B510
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001154 Genomic DNA. Translation: ACO75065.1.
    RefSeqiYP_002796074.1. NC_012559.1.

    Genome annotation databases

    EnsemblBacteriaiACO75065; ACO75065; LHK_02081.
    GeneIDi7757694.
    KEGGilhk:LHK_02081.
    PATRICi22301017. VBILarHon49832_1897.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001154 Genomic DNA. Translation: ACO75065.1 .
    RefSeqi YP_002796074.1. NC_012559.1.

    3D structure databases

    ProteinModelPortali C1D9G3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557598.LHK_02081.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACO75065 ; ACO75065 ; LHK_02081 .
    GeneIDi 7757694.
    KEGGi lhk:LHK_02081.
    PATRICi 22301017. VBILarHon49832_1897.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi CACKVER.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci LHON557598:GHO5-2111-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats."
      Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y.
      PLoS Genet. 5:E1000416-E1000416(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HLHK9.

    Entry informationi

    Entry nameiNAPA_LARHH
    AccessioniPrimary (citable) accession number: C1D9G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3