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C1D9G3

- NAPA_LARHH

UniProt

C1D9G3 - NAPA_LARHH

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi56 – 561Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi60 – 601Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi88 – 881Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciLHON557598:GHO5-2111-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:LHK_02081
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
ProteomesiUP000002010: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Tat-type signalUniRule annotationAdd
BLAST
Chaini35 – 839805Periplasmic nitrate reductasePRO_1000186366Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi557598.LHK_02081.

Structurei

3D structure databases

ProteinModelPortaliC1D9G3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 102574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCACKVER.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C1D9G3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLTRRDFIK ANAAAAAATA AAVNLPLVPS MAQAATTDPA TAGADIKWDK
60 70 80 90 100
AACRFCGTGC SVLVGTKGGR VVATQGDPDA PVNRGLNCIK GYFLSKIMYG
110 120 130 140 150
EDRLTKPLLR MKNGKFDKNG EFTPVTWKQA FDVMEEKFKT AMKAGGPEAV
160 170 180 190 200
AMFGSGQWTI WEGYAAAKFM KAGLRSNNLD PNARHCMASA VVGFMRTFNM
210 220 230 240 250
DEPMGCYDDI EKADAFVLWG SNMAEMHPIL WSRITDRRLT HPDCQVHVLS
260 270 280 290 300
TYEHRSFELA DNKLVFGPQT DLAILNYIAN HIIQTGAVNK DFIQKYCAFM
310 320 330 340 350
KGDTDIGYGL RPTHPKQKAA KNPDSGKMDP ITFEQFAAFV KPYTLEYTAR
360 370 380 390 400
TSCVPAERLK RLAELYADPK KKVVSFWTMG FNQHTRGTWA NNLVYNIHLL
410 420 430 440 450
TGKISTPGCG PFSLTGQPSA CGTAREVGTF AHRLPADLVV MNPEHRKIAE
460 470 480 490 500
NIWKLPEGTI PPKMGLHAVA MQRALKDSKL KVYWQQCNNN MQAGPNINEE
510 520 530 540 550
MYPGWRNPET FIIVSDPYPT VSAMAADLIL PTAMWVEKEG AYGNAERRTQ
560 570 580 590 600
FWRQQVNAPG EAKSDLWQVV EFSKRFKVEE VWPAELVAKK PEYRGKTLYD
610 620 630 640 650
VLFANGQVNK YKLDDMARQH GTAYHNEEAR HVGFYLQKGL FEEYAAFGRG
660 670 680 690 700
HGHDLALFDT YHKARGLRWP VVDGKETLWR YREGFDPYVK KGEGVRFYGQ
710 720 730 740 750
KDGRARIIAL PFEPAAEVPD REYNMWLCTG RVLEHWHTGS MTRRVPELYR
760 770 780 790 800
AVPDAQVFMH PADAELRGLK RGQQVKVISR RGEITLTLET RGRNKPPQGL
810 820 830
VFIPFFDEGR LVNKLTLDAT CPLSKETDYK KCAVKVVRA
Length:839
Mass (Da):94,304
Last modified:May 26, 2009 - v1
Checksum:i1C02424A6F70B510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA. Translation: ACO75065.1.
RefSeqiWP_012697551.1. NC_012559.1.
YP_002796074.1. NC_012559.1.

Genome annotation databases

EnsemblBacteriaiACO75065; ACO75065; LHK_02081.
GeneIDi7757694.
KEGGilhk:LHK_02081.
PATRICi22301017. VBILarHon49832_1897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA. Translation: ACO75065.1 .
RefSeqi WP_012697551.1. NC_012559.1.
YP_002796074.1. NC_012559.1.

3D structure databases

ProteinModelPortali C1D9G3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557598.LHK_02081.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO75065 ; ACO75065 ; LHK_02081 .
GeneIDi 7757694.
KEGGi lhk:LHK_02081.
PATRICi 22301017. VBILarHon49832_1897.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi CACKVER.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci LHON557598:GHO5-2111-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats."
    Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y.
    PLoS Genet. 5:E1000416-E1000416(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLHK9.

Entry informationi

Entry nameiNAPA_LARHH
AccessioniPrimary (citable) accession number: C1D9G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3