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C1D9G3 (NAPA_LARHH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:LHK_02081
OrganismLaribacter hongkongensis (strain HLHK9) [Complete proteome] [HAMAP]
Taxonomic identifier557598 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter

Protein attributes

Sequence length839 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434Tat-type signal Potential
Chain35 – 839805Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186366

Regions

Domain46 – 102574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding561Iron-sulfur (4Fe-4S) By similarity
Metal binding601Iron-sulfur (4Fe-4S) By similarity
Metal binding881Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
C1D9G3 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 1C02424A6F70B510

FASTA83994,304
        10         20         30         40         50         60 
MTLTRRDFIK ANAAAAAATA AAVNLPLVPS MAQAATTDPA TAGADIKWDK AACRFCGTGC 

        70         80         90        100        110        120 
SVLVGTKGGR VVATQGDPDA PVNRGLNCIK GYFLSKIMYG EDRLTKPLLR MKNGKFDKNG 

       130        140        150        160        170        180 
EFTPVTWKQA FDVMEEKFKT AMKAGGPEAV AMFGSGQWTI WEGYAAAKFM KAGLRSNNLD 

       190        200        210        220        230        240 
PNARHCMASA VVGFMRTFNM DEPMGCYDDI EKADAFVLWG SNMAEMHPIL WSRITDRRLT 

       250        260        270        280        290        300 
HPDCQVHVLS TYEHRSFELA DNKLVFGPQT DLAILNYIAN HIIQTGAVNK DFIQKYCAFM 

       310        320        330        340        350        360 
KGDTDIGYGL RPTHPKQKAA KNPDSGKMDP ITFEQFAAFV KPYTLEYTAR TSCVPAERLK 

       370        380        390        400        410        420 
RLAELYADPK KKVVSFWTMG FNQHTRGTWA NNLVYNIHLL TGKISTPGCG PFSLTGQPSA 

       430        440        450        460        470        480 
CGTAREVGTF AHRLPADLVV MNPEHRKIAE NIWKLPEGTI PPKMGLHAVA MQRALKDSKL 

       490        500        510        520        530        540 
KVYWQQCNNN MQAGPNINEE MYPGWRNPET FIIVSDPYPT VSAMAADLIL PTAMWVEKEG 

       550        560        570        580        590        600 
AYGNAERRTQ FWRQQVNAPG EAKSDLWQVV EFSKRFKVEE VWPAELVAKK PEYRGKTLYD 

       610        620        630        640        650        660 
VLFANGQVNK YKLDDMARQH GTAYHNEEAR HVGFYLQKGL FEEYAAFGRG HGHDLALFDT 

       670        680        690        700        710        720 
YHKARGLRWP VVDGKETLWR YREGFDPYVK KGEGVRFYGQ KDGRARIIAL PFEPAAEVPD 

       730        740        750        760        770        780 
REYNMWLCTG RVLEHWHTGS MTRRVPELYR AVPDAQVFMH PADAELRGLK RGQQVKVISR 

       790        800        810        820        830 
RGEITLTLET RGRNKPPQGL VFIPFFDEGR LVNKLTLDAT CPLSKETDYK KCAVKVVRA 

« Hide

References

[1]"The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats."
Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y.
PLoS Genet. 5:E1000416-E1000416(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HLHK9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001154 Genomic DNA. Translation: ACO75065.1.
RefSeqYP_002796074.1. NC_012559.1.

3D structure databases

ProteinModelPortalC1D9G3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557598.LHK_02081.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACO75065; ACO75065; LHK_02081.
GeneID7757694.
KEGGlhk:LHK_02081.
PATRIC22301017. VBILarHon49832_1897.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMACACKVER.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycLHON557598:GHO5-2111-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_LARHH
AccessionPrimary (citable) accession number: C1D9G3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families