C1D558 (ISPD_LARHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	LHK_00882

Organism

Laribacter hongkongensis (strain HLHK9) [Complete proteome] [HAMAP]

Taxonomic identifier

557598 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Laribacter

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000191060
Sites
	Site	16	1	Transition state stabilizer By similarity
	Site	23	1	Transition state stabilizer By similarity
	Site	155	1	Positions MEP for the nucleophilic attack By similarity
	Site	209	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1D558 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: EDBCF48F5EB72C32	FASTA	230	24,849
	10 20 30 40 50 60 MSRCLALVPA AGGGSRMGAD RPKQYLDLAG APLLAHTLRR LLAEPRLARV LVVLAPDDVW 70 80 90 100 110 120 FDRFDWPRDV RLEILRVGGA TRAESVRNGL LHAGAAADDW VLVHDAARCC LPPDALDRLI 130 140 150 160 170 180 DTLQADPVGG LLALPVADTL KRETSGQRVA QTVSREGLWL AQTPQMFRAG MLALALDRPL 190 200 210 220 230 DRAVTDEASA IERLGLVPRL VTGDALNFKV TWPHDLVLAR AVLGLDNAGK

References

[1]

"The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats." Woo P.C.Y., Lau S.K.P., Tse H., Teng J.L.L., Curreem S.O., Tsang A.K.L., Fan R.Y.Y., Wong G.K.M., Huang Y., Loman N.J., Snyder L.A.S., Cai J.J., Huang J.-D., Mak W., Pallen M.J., Lok S., Yuen K.-Y. PLoS Genet. 5:E1000416-E1000416(2009) [PubMed: 19283063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HLHK9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001154 Genomic DNA. Translation: ACO73875.1.
RefSeq	YP_002794884.1. NC_012559.1.
3D structure databases
ProteinModelPortal	C1D558.
ModBase	Search...
Protein-protein interaction databases
STRING	C1D558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7756505.
GenomeReviews	Gene locus LHK_00882 in contig CP001154_GR.
KEGG	lhk:LHK_00882.
PATRIC	22298866. VBILarHon49832_0836.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FPQGAIL.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_LARHH

Accession

Primary (citable) accession number: C1D558

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program