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Protein

Enolase

Gene

eno

Organism
Deinococcus deserti (strain VCD115 / DSM 17065 / LMG 22923)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei153SubstrateUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Active sitei203Proton donorUniRule annotation1
Metal bindingi240MagnesiumUniRule annotation1
Metal bindingi283MagnesiumUniRule annotation1
Binding sitei283SubstrateUniRule annotation1
Metal bindingi310MagnesiumUniRule annotation1
Binding sitei310SubstrateUniRule annotation1
Active sitei335Proton acceptorUniRule annotation1
Binding sitei335Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei386SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Deide_00030
OrganismiDeinococcus deserti (strain VCD115 / DSM 17065 / LMG 22923)
Taxonomic identifieri546414 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002208 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002050871 – 422EnolaseAdd BLAST422

Proteomic databases

PaxDbiC1CXJ3.
PRIDEiC1CXJ3.

Interactioni

Protein-protein interaction databases

STRINGi546414.Deide_00030.

Structurei

3D structure databases

ProteinModelPortaliC1CXJ3.
SMRiC1CXJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni362 – 365Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiDQTLICG.
OrthoDBiPOG091H02DK.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

C1CXJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIEKVIARE VLDSRGNPTV EAEVHLDSGF VGRAIVPSGA STGTHEALEL
60 70 80 90 100
RDGGSRYMGK GVQQAVRNVE EALGPAIVGL DASEQIAIDA ALMAVDGTPN
110 120 130 140 150
KGKMGGNAIL AVSLATSRAA AEELGVPLYR YLGGSNAKTL PVPMMNLING
160 170 180 190 200
GAHADNSVDF QEFMVMPIGA PTFREALRYG TETFHSLKKV LSSRGYNTNV
210 220 230 240 250
GDEGGFAPDL KSNEEALQVL LEAIEKAGYE PGKDIAIALD PAVTELYKDG
260 270 280 290 300
HYDLESEGRT LSTAEMVDFW ADWADRYPIV SIEDGLAEDD WDGWQALTTK
310 320 330 340 350
IGDRVQLVGD DLFVTNPERL QRGIDTGVGN AILVKVNQIG SLTESMDAIE
360 370 380 390 400
LAKRHHYGTI ISHRSGESED SFIADLAVAT NAGQIKTGSA SRSDRIAKYN
410 420
QLLRIEHALG DRAVYLGRKA LR
Length:422
Mass (Da):45,338
Last modified:May 26, 2009 - v1
Checksum:iC7D35CE287561C81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001114 Genomic DNA. Translation: ACO44799.1.
RefSeqiWP_012691922.1. NC_012526.1.

Genome annotation databases

EnsemblBacteriaiACO44799; ACO44799; Deide_00030.
KEGGiddr:Deide_00030.
PATRICi21613354. VBIDeiDes121019_0003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001114 Genomic DNA. Translation: ACO44799.1.
RefSeqiWP_012691922.1. NC_012526.1.

3D structure databases

ProteinModelPortaliC1CXJ3.
SMRiC1CXJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi546414.Deide_00030.

Proteomic databases

PaxDbiC1CXJ3.
PRIDEiC1CXJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACO44799; ACO44799; Deide_00030.
KEGGiddr:Deide_00030.
PATRICi21613354. VBIDeiDes121019_0003.

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiDQTLICG.
OrthoDBiPOG091H02DK.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_DEIDV
AccessioniPrimary (citable) accession number: C1CXJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: March 15, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.