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C1CV55 (C1CV55_DEIDV) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Deide_11660
OrganismDeinococcus deserti (strain VCD115 / DSM 17065 / LMG 22923) [Complete proteome] [HAMAP]
Taxonomic identifier546414 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length1066 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
C1CV55 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 29D6AD569DFC02F9

FASTA1,066121,169
        10         20         30         40         50         60 
MTVTPKPPMF GTVPTNPSFP ELELQTLRWW QERRIFERSL EQTADGPRFT FYEGPPTANG 

        70         80         90        100        110        120 
MPGIHHVEAR SLKDLFPRFK TMQGYFVGRK AGWDTHGLPV EIAVEKKLGL NSKREVEAYG 

       130        140        150        160        170        180 
IDKFNEQCRE TVFEYEHEWR RFTERMAYWV DLDRPYMTLQ RNYIESIWWS VQQLDQKGLL 

       190        200        210        220        230        240 
YKGFRVAPYC PKDGTTLSNA EVSEGYKDIQ DPSVYVPFRL TDPAALDLPD HTAFLVWTTT 

       250        260        270        280        290        300 
PWTLPYNVGV AIHPDLEYVA ARDKDGRVLI LAASLRAEVL GEDAEIVRTF RGSELERVAY 

       310        320        330        340        350        360 
EPLFTEAYEA EGEGKTVWMS GLDTYVSDKD GTGIVHTAPA FGEDDMRLAR NYGFPVIVGV 

       370        380        390        400        410        420 
DTEGKHRFGP WKGVFFRDAN TEIVRDLRAR GLMWKEKNFL HSYPHCWRCG TPLMYYATES 

       430        440        450        460        470        480 
WYLNNTRLKD RLIELNGTID WHPAHIRTGR YGGWLENLID WNISRSRYWG TPLPVWEAED 

       490        500        510        520        530        540 
GEYRVIGSYA ELAELSGRPE VTGESFDPHR PYVDDITFEA DGKTFRRVPY VMDVWYDSGS 

       550        560        570        580        590        600 
MPFAQHHYPF ENKELFEQGG FPADYISEAI DQTRGWFNSL HQIGTMVFDS VAYKSVICAG 

       610        620        630        640        650        660 
HFLDEKGQKM SKSKGNILDP WEVFNTYGAD AARWYTYVSA PPELSRRVGL NVIGESFRSY 

       670        680        690        700        710        720 
FMTLWNTYSF FVLYANLDQP DLRAAPPVEE RPEVDRWLIA KVQALVGTVT GALNNYDPTG 

       730        740        750        760        770        780 
SSRALQDFVV EDLSNWYVRR NRRRFWSGDG QVDLSAYATL HYALVTVTQL TAPFTPFLAE 

       790        800        810        820        830        840 
SLYQNLVRSV TPDAPESVHL ATWPQVDEAL AAPSLVGEMD AVLRVVSLGR AVRGQTGMRQ 

       850        860        870        880        890        900 
RQPLPKVMLR ARSAEQTAAL GRFVEQIKEE LNVKEVELLD QYAELVSYQL RPNLPLLGKK 

       910        920        930        940        950        960 
FGKAVPQVRA ALQEADASEV ARFVRDGKFF EVVAPTGERF ELGPDEVLVD ARSPEGFAAQ 

       970        980        990       1000       1010       1020 
EEAGYLVAFD TQLTRELELE GLARDLVRGI QDGRKKAGFE VSDRITLHLD LTGDAREAAE 

      1030       1040       1050       1060 
AWQEYLMSET LTESLVFGAA EGFAAEVEGG TAYLERLERD GSMAGA

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References

[1]"Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti."
de Groot A., Dulermo R., Ortet P., Blanchard L., Guerin P., Fernandez B., Vacherie B., Dossat C., Jolivet E., Siguier P., Chandler M., Barakat M., Dedieu A., Barbe V., Heulin T., Sommer S., Achouak W., Armengaud J.
PLoS Genet. 5:E1000434-E1000434(2009) [PubMed: 19370165] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VCD115 / DSM 17065 / LMG 22923.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001114 Genomic DNA. Translation: ACO46072.1.
RefSeqYP_002785826.1. NC_012526.1.

3D structure databases

ProteinModelPortalC1CV55.
ModBaseSearch...

Protein-protein interaction databases

STRINGC1CV55.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7738390.
GenomeReviewsGene locus Deide_11660 in contig CP001114_GR.
KEGGddr:Deide_11660.
PATRIC21616096. VBIDeiDes121019_1329.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC1CV55_DEIDV
AccessionPrimary (citable) accession number: C1CV55
Entry history
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info