C1CLX1 (SYT_STRZP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	SPP_1650

Organism

Streptococcus pneumoniae (strain P1031) [Complete proteome] [HAMAP]

Taxonomic identifier

488223 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Threonine--tRNA ligase HAMAP-Rule MF_00184		PRO_1000199572
Regions
	Region	242 – 540	299	Catalytic HAMAP-Rule MF_00184
Sites
	Metal binding	336	1	Zinc; catalytic By similarity
	Metal binding	387	1	Zinc; catalytic By similarity
	Metal binding	517	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1CLX1 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 311E63158C457877	FASTA	647	74,664
	10 20 30 40 50 60 MINITFPDGA VREFESGVTT FEIAQSISNS LAKKALAGKF NGKLIDTTRA ITEDGSIEIV 70 80 90 100 110 120 TPDHEDALPI LRHSAAHLFA QAARRLFPDI HLGVGPAIED GFYYDTDNTA GQISNEDLPR 130 140 150 160 170 180 IEEEMQKIVK ENFPSIREEV TKDEAREIFK NDPYKLELIE EHSEDEGGLT IYRQGEYVDL 190 200 210 220 230 240 CRGPHVPSTG RIQIFHLLHV AGAYWRGNSD NAMMQRIYGT AWFDKKDLKN YLQMREEAKE 250 260 270 280 290 300 RDHRKLGKEL DLFIISQEVG QGLPFWLPNG ATIRRELERY IVNKELASGY QHVYTPPLAS 310 320 330 340 350 360 VELYKTSGHW DHYQEDMFPT MDMGDGEEFV LRPMNCPHHI QVFKHHVHSY RELPIRIAEI 370 380 390 400 410 420 GMMHRYEKSG ALTGLQRVRE MSLNDGHLFV TPEQIQEEFQ RALQLIIDVY EDFNLTDYRF 430 440 450 460 470 480 RLSLRDPQDT HKYFDNDEMW ENAQTMLRAA LDEMGVNYFE AEGEAAFYGP KLDIQIKTAL 490 500 510 520 530 540 GKEETLSTIQ LDFLLPERFD LKYIGADGED HRPVMIHRGV ISTMERFTAI LIENYKGAFP 550 560 570 580 590 600 TWLAPHQVTL IPVSNEKHVD YAWEVAKKLR DRGVRADVDE RNEKMQFKIR ASQTSKIPYQ 610 620 630 640 LIVGDKEMED ETVNVRRYGQ KETQTVSVDN FVQAILADIA NKSRVEK

References

[1]	"Complete genome sequence of Streptococcus pneumoniae strain P1031." Hotopp J.D., Censini S., Masignani V., Covacci A., Tettelin H. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: P1031.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000920 Genomic DNA. Translation: ACO21023.1.
RefSeq	YP_002738759.1. NC_012467.1.
3D structure databases
ProteinModelPortal	C1CLX1.
ModBase	Search...
Protein-protein interaction databases
STRING	488223.SPP_1650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO21023; ACO21023; SPP_1650.
GeneID	7681679.
KEGG	spp:SPP_1650.
PATRIC	32460631. VBIStrPne91701_1711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HOG000003879.
KO	K01868.
OMA	GRKWQLG.
ProtClustDB	PRK00413.
Family and domain databases
Gene3D	3.10.20.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_00184. Thr_tRNA_synth.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_dom. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-ligase_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_STRZP

Accession

Primary (citable) accession number: C1CLX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program