Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C1C8P8

- SYI_STRP7

UniProt

C1C8P8 - SYI_STRP7

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus pneumoniae (strain 70585)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPNE488221:GH4U-1659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SP70585_1699
OrganismiStreptococcus pneumoniae (strain 70585)
Taxonomic identifieri488221 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002211: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000189202Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi488221.SP70585_1699.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1C8P8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKDTLNLG KTAFPMRAGL PTKEPVWQKE WEDAKLYQRR QELNEGKPHF
60 70 80 90 100
VLHDGPPYAN GNIHVGHAMN HISKDIIIRS KSMSGFNAPY IPGWDTHGLP
110 120 130 140 150
IEQVLAKQGV KRKEMDLVEY LKLCREYALS QVYKQRDDFK RLGMSGDWEN
160 170 180 190 200
LYVTLTPDYE AAQIRVFGEM ANKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDLVSTSLYY ANKVKDGKGI LDTDTYIVVW TTTPFTITAS RGLTVGADID
260 270 280 290 300
YVLVQPAGET RKFVVAAELL TSLSEKFGWA DVQVLATYRG QELNHIVTEH
310 320 330 340 350
PWDTAVDELV ILGDHVTTDS GTGIVHTAPG FGEDDYNVGI ANGLEVAVTV
360 370 380 390 400
DERGIMMANA GPEFEGQFYD KVVPTVIEKL GNLLLAQEEI SHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSKFR QEILDAIDKV KFHTEWGKVR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED GTAIMTAETI EHVAQLFEVH GSSIWWERDA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFKK ETDIMDVWFD SGSSWNGVLV NRPNLTYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VANHGVAPYK QILSQGFALD GKGEKMSKSL
610 620 630 640 650
GNTIAPSDVE KQFGAEILRL WVTSVDSSND VRISMDILSQ VSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPAQDTVA YDELRSVDKY MTIRFNQLVK TIRDAYADFE
710 720 730 740 750
FLTIYKALVN FINVDLSAFY LDFAKDVVYI EGAKSLERRQ MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTAEEIWSY LEFETEDFVQ LSELPEAQTF ANQEEILDTW
810 820 830 840 850
AAFMDFRGQA QKALEEARNA KVIGKSLEAH LTVYQNEVVK TLLEAVNSNV
860 870 880 890 900
AQLLIVSELT IVEGPAPEAA LSFEDVAFTV ERATGEVCDR CRRIDPTTAE
910 920 930
RSYQAVICDH CASIVEENFA EAVAEGFEEK
Length:930
Mass (Da):105,212
Last modified:May 26, 2009 - v1
Checksum:i769BFCB2D2C95C88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000918 Genomic DNA. Translation: ACO16812.1.
RefSeqiYP_002740924.1. NC_012468.1.

Genome annotation databases

EnsemblBacteriaiACO16812; ACO16812; SP70585_1699.
GeneIDi7684789.
KEGGisnm:SP70585_1699.
PATRICi19669779. VBIStrPne83895_1752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000918 Genomic DNA. Translation: ACO16812.1 .
RefSeqi YP_002740924.1. NC_012468.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 488221.SP70585_1699.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO16812 ; ACO16812 ; SP70585_1699 .
GeneIDi 7684789.
KEGGi snm:SP70585_1699.
PATRICi 19669779. VBIStrPne83895_1752.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPNE488221:GH4U-1659-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Streptococcus pneumoniae strain 70585."
    Hotopp J.D., Censini S., Masignani V., Covacci A., Tettelin H.
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70585.

Entry informationi

Entry nameiSYI_STRP7
AccessioniPrimary (citable) accession number: C1C8P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3