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C1C6R3 (PROB_STRP7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:SP70585_0970
OrganismStreptococcus pneumoniae (strain 70585) [Complete proteome] [HAMAP]
Taxonomic identifier488221 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000193704

Regions

Domain275 – 35581PUA
Nucleotide binding168 – 1692ATP By similarity
Nucleotide binding210 – 2167ATP By similarity

Sites

Binding site91ATP By similarity
Binding site491Substrate By similarity
Binding site1361Substrate By similarity
Binding site1481Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
C1C6R3 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 4E2B950B4239C1C3

FASTA36939,873
        10         20         30         40         50         60 
MKYKRIVFKV GTSSLTNEDG SLSRSKVKDI TQQLAMLHEA GHELILVSSG AIAAGFGALG 

        70         80         90        100        110        120 
FKKRPTKIAD KQASAAVGQG LLLEEYTTNL LLRQIVSAQI LLTQDDFVDK RRYKNAHQAL 

       130        140        150        160        170        180 
SVLLNRGAIP IINENDSVVI DELKVGDNDT LSAQVAAMVQ ADLLVFLTDV DGLYTGNPNS 

       190        200        210        220        230        240 
DPRAKRLERI ETINREIIDM AGGAGSSNGT GGMLTKIKAA TIATESGVPV YICSSLKSDS 

       250        260        270        280        290        300 
MIEAAEETED GSYFVAQEKG LRTQKQWLAF YAQSQGSIWV DKGAAEALSQ HGKSLLLSGI 

       310        320        330        340        350        360 
VEAEGAFSYG DIVTVFDKES GKSLGKGRVQ FGASALEDML RSQKAKGVLI YRDDWISITP 


EIQLLFTEF 

« Hide

References

[1]"Complete genome sequence of Streptococcus pneumoniae strain 70585."
Hotopp J.D., Censini S., Masignani V., Covacci A., Tettelin H.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 70585.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000918 Genomic DNA. Translation: ACO17668.1.
RefSeqYP_002740239.1. NC_012468.1.

3D structure databases

ProteinModelPortalC1C6R3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING488221.SP70585_0970.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACO17668; ACO17668; SP70585_0970.
GeneID7683259.
KEGGsnm:SP70585_0970.
PATRIC19668289. VBIStrPne83895_1009.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246369.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycSPNE488221:GH4U-954-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_STRP7
AccessionPrimary (citable) accession number: C1C6R3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways