C1BA07 (C1BA07_RHOOB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAH52510.1
Ordered Locus Names:	ROP_42630

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C1BA07 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 8C71824BA6176E73	FASTA	973	106,106
	10 20 30 40 50 60 MAKGDNGSAQ GAAGASGQPR RLVIVESPTK ARKIAPYLGK NYVVEASVGH IRDLPRGAAD 70 80 90 100 110 120 VPAKYKGESW ARLGVNVDQD FEPLYVVSPE KKSKVTELKS LLKDADELFL ATDPDREGEA 130 140 150 160 170 180 IAWHLLETLK PKIPVRRMVF HEITEPAIRA AAEDTRELDN DLVDAQETRR ILDRLYGYEV 190 200 210 220 230 240 SPVLWKKVMP RLSAGRVQSV ATRVIVQRER ERMAFRSASY WDISATLDAG ADASPRSFGA 250 260 270 280 290 300 RLVSVDGSRV ATGRDFGSDG QLKTSTVTVL DEARAQRLAE SLAGVDLTVS SAESKPYTRK 310 320 330 340 350 360 PYAPFMTSTL QQEAGRKLRF TSERTMRIAQ RLYENGYITY MRTDSTTLSS SAIAAARAQA 370 380 390 400 410 420 TELYGAEYVH DTPRQYTRKV KNAQEAHEAI RPAGDVFQTP GQLHSRLDTD EFRLYELIWQ 430 440 450 460 470 480 RTVASQMADA RGTTLTLRIT GTAGTGEECT FSSSGRTITF AGFLKAYVES VDEEAGGQSD 490 500 510 520 530 540 DAESRLPVLV EGQAVTATKL DPDGHTTNPP ARYTEASLIK TLEELGIGRP STYSSIIKTI 550 560 570 580 590 600 LDRGYVYKRG SALVPSWVAF AVIGLLEMHF GRLVDFDFTA GMEDDLDAIA GGREQRGNWL 610 620 630 640 650 660 SSFYFGGDHG AEGSVAREGG LKKMVGVNLE EIDAREVNSI RLFDDAEGRE VHVRVGRFGP 670 680 690 700 710 720 YLERMVQNPD DPEGDLISQR ANLPDDLPPD ELTPEYAEKL FSTPQEGRKL GVDPLTGHEI 730 740 750 760 770 780 VAKEGRFGPY VTEILPEPEP EPEPAVVPVT DESGDGTTKT KTAAAKKAPA KKAAKKATGP 790 800 810 820 830 840 KPRTGSLLKS MDLATVTLDD ALKLLSLPRV VGVDPESKEE ITAQNGRYGP YLKKGTDSRS 850 860 870 880 890 900 LATEDQMFTV TLDEALKIYA EPKRRGRQAA ATPPLRELGV DSVSEKPMVI KDGRFGPYVT 910 920 930 940 950 960 DGETNASLRK GDEVESITDA RASELLADRR ARGPVKKKAA AKKAPAKKAA KKTAAKKAPA 970 KKAASKKAAD KKA

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH52510.1.
RefSeq	YP_002781455.1. NC_012522.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1BA07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7741136.
GenomeReviews	Gene locus ROP_42630 in contig AP011115_GR.
KEGG	rop:ROP_42630.
PATRIC	23225837. VBIRhoOpa21106_4300.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1BA07_RHOOB

Accession

Primary (citable) accession number: C1BA07

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)