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C1B4K9

- C1B4K9_RHOOB

UniProt

C1B4K9 - C1B4K9_RHOOB

Protein

Riboflavin biosynthesis protein RibBA

Gene

ribA

Organism
Rhodococcus opacus (strain B4)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
    Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotationSAAS annotation

    Catalytic activityi

    D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
    GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation
    Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi29 – 291Magnesium or manganese 1UniRule annotation
    Metal bindingi29 – 291Magnesium or manganese 2UniRule annotation
    Binding sitei33 – 331D-ribulose 5-phosphateUniRule annotation
    Sitei127 – 1271Essential for DHBP synthase activityUniRule annotation
    Metal bindingi144 – 1441Magnesium or manganese 2UniRule annotation
    Binding sitei165 – 1651D-ribulose 5-phosphateUniRule annotation
    Sitei165 – 1651Essential for DHBP synthase activityUniRule annotation
    Metal bindingi264 – 2641Zinc; catalyticUniRule annotation
    Metal bindingi275 – 2751Zinc; catalyticUniRule annotation
    Metal bindingi277 – 2771Zinc; catalyticUniRule annotation
    Binding sitei280 – 2801GTPUniRule annotation
    Binding sitei325 – 3251GTPUniRule annotation
    Active sitei337 – 3371Proton acceptor; for GTP cyclohydrolase activityUniRule annotation
    Active sitei339 – 3391Nucleophile; for GTP cyclohydrolase activityUniRule annotation
    Binding sitei360 – 3601GTPUniRule annotation
    Binding sitei365 – 3651GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi259 – 2635GTPUniRule annotation
    Nucleotide bindingi303 – 3053GTPUniRule annotation

    GO - Molecular functioni

    1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-KW
    3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP
    6. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. riboflavin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationImported, LyaseUniRule annotation

    Keywords - Biological processi

    Riboflavin biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciROPA632772:GH0Q-7007-MONOMER.
    UniPathwayiUPA00275; UER00399.
    UPA00275; UER00400.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin biosynthesis protein RibBAUniRule annotation
    Gene namesi
    Name:ribAImported
    Synonyms:ribBAUniRule annotation
    Ordered Locus Names:ROP_69510Imported
    OrganismiRhodococcus opacus (strain B4)Imported
    Taxonomic identifieri632772 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000002212: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi632772.ROP_69510.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 204204DHBP synthaseUniRule annotationAdd
    BLAST
    Regioni28 – 292D-ribulose 5-phosphate bindingUniRule annotation
    Regioni141 – 1455D-ribulose 5-phosphate bindingUniRule annotation
    Regioni205 – 417213GTP cyclohydrolase IIUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
    In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0108.
    HOGENOMiHOG000115440.
    KOiK14652.
    OMAiFDTIERA.
    OrthoDBiEOG679TK8.

    Family and domain databases

    Gene3Di3.90.870.10. 1 hit.
    HAMAPiMF_00179. RibA.
    MF_00180. RibB.
    MF_01283. RibBA.
    InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
    IPR000422. DHBP_synthase_RibB.
    IPR000926. GTP_CycHdrlaseII_RibA.
    IPR016299. Riboflavin_synth_RibBA.
    [Graphical view]
    PfamiPF00926. DHBP_synthase. 1 hit.
    PF00925. GTP_cyclohydro2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001259. RibA. 1 hit.
    SUPFAMiSSF55821. SSF55821. 1 hit.
    TIGRFAMsiTIGR00505. ribA. 1 hit.
    TIGR00506. ribB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C1B4K9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRFDTIERA VADIAAGKAV VVVDDEDREN EGDLIFAAEK ATPELVAFMV    50
    RYTSGYLCVP LDGADCDRLG LPPMYATNQD KHGTAYTVTV DAREGIGTGI 100
    SASDRAATMR LLADPSSGAQ DFTRPGHVVP LRAKEGGVLR RPGHTEAAVD 150
    LARMADLRPA GVICEIVSQK DEGHMAQTDE LRVFADDHDL ALISIADLIA 200
    WRRKHEKHVE RVASARIPTR HGEFTAVGYR SIYDDVEHVA LVRGDLPGPD 250
    GDGSDVLVRV HSECLTGDVF GSLRCDCGPQ LDAALDMVAQ EGRGVVLYMR 300
    GHEGRGIGLM HKLQAYQLQD AGSDTVDANL ELGLPADARD YGIGAQILVD 350
    LGISSMRLLT NNPAKRVGLD GYGLQITERV SMPLRANAEN LTYLRTKRDR 400
    MGHDLIGLDD FEAGEML 417
    Length:417
    Mass (Da):45,184
    Last modified:May 26, 2009 - v1
    Checksum:i96C9BD0ECEC2F875
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP011115 Genomic DNA. Translation: BAH55198.1.
    RefSeqiYP_002784143.1. NC_012522.1.

    Genome annotation databases

    EnsemblBacteriaiBAH55198; BAH55198; ROP_69510.
    GeneIDi7744531.
    KEGGirop:ROP_69510.
    PATRICi23231355. VBIRhoOpa21106_7036.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP011115 Genomic DNA. Translation: BAH55198.1 .
    RefSeqi YP_002784143.1. NC_012522.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 632772.ROP_69510.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH55198 ; BAH55198 ; ROP_69510 .
    GeneIDi 7744531.
    KEGGi rop:ROP_69510.
    PATRICi 23231355. VBIRhoOpa21106_7036.

    Phylogenomic databases

    eggNOGi COG0108.
    HOGENOMi HOG000115440.
    KOi K14652.
    OMAi FDTIERA.
    OrthoDBi EOG679TK8.

    Enzyme and pathway databases

    UniPathwayi UPA00275 ; UER00399 .
    UPA00275 ; UER00400 .
    BioCyci ROPA632772:GH0Q-7007-MONOMER.

    Family and domain databases

    Gene3Di 3.90.870.10. 1 hit.
    HAMAPi MF_00179. RibA.
    MF_00180. RibB.
    MF_01283. RibBA.
    InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
    IPR000422. DHBP_synthase_RibB.
    IPR000926. GTP_CycHdrlaseII_RibA.
    IPR016299. Riboflavin_synth_RibBA.
    [Graphical view ]
    Pfami PF00926. DHBP_synthase. 1 hit.
    PF00925. GTP_cyclohydro2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001259. RibA. 1 hit.
    SUPFAMi SSF55821. SSF55821. 1 hit.
    TIGRFAMsi TIGR00505. ribA. 1 hit.
    TIGR00506. ribB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
      Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
      Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B4Imported.

    Entry informationi

    Entry nameiC1B4K9_RHOOB
    AccessioniPrimary (citable) accession number: C1B4K9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 26, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported, Multifunctional enzymeUniRule annotation

    External Data

    Dasty 3