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C1B4K9

- C1B4K9_RHOOB

UniProt

C1B4K9 - C1B4K9_RHOOB

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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribA

Organism
Rhodococcus opacus (strain B4)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotationSAAS annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Note: Binds 1 zinc ion per subunit.UniRule annotation
  • Note: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Magnesium or manganese 1UniRule annotation
Metal bindingi29 – 291Magnesium or manganese 2UniRule annotation
Binding sitei33 – 331D-ribulose 5-phosphateUniRule annotation
Sitei127 – 1271Essential for DHBP synthase activityUniRule annotation
Metal bindingi144 – 1441Magnesium or manganese 2UniRule annotation
Binding sitei165 – 1651D-ribulose 5-phosphateUniRule annotation
Sitei165 – 1651Essential for DHBP synthase activityUniRule annotation
Metal bindingi264 – 2641Zinc; catalyticUniRule annotation
Metal bindingi275 – 2751Zinc; catalyticUniRule annotation
Metal bindingi277 – 2771Zinc; catalyticUniRule annotation
Binding sitei280 – 2801GTPUniRule annotation
Binding sitei325 – 3251GTPUniRule annotation
Active sitei337 – 3371Proton acceptor; for GTP cyclohydrolase activityUniRule annotation
Active sitei339 – 3391Nucleophile; for GTP cyclohydrolase activityUniRule annotation
Binding sitei360 – 3601GTPUniRule annotation
Binding sitei365 – 3651GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi259 – 2635GTPUniRule annotation
Nucleotide bindingi303 – 3053GTPUniRule annotation

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
  6. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported, LyaseUniRule annotation

Keywords - Biological processi

Riboflavin biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotation

Enzyme and pathway databases

BioCyciROPA632772:GH0Q-7007-MONOMER.
UniPathwayiUPA00275; UER00399.
UPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotationSAAS annotation
Gene namesi
Name:ribAImported
Synonyms:ribBAUniRule annotation
Ordered Locus Names:ROP_69510Imported
OrganismiRhodococcus opacus (strain B4)Imported
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000002212: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi632772.ROP_69510.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 204204DHBP synthaseUniRule annotationAdd
BLAST
Regioni28 – 292D-ribulose 5-phosphate bindingUniRule annotation
Regioni141 – 1455D-ribulose 5-phosphate bindingUniRule annotation
Regioni205 – 417213GTP cyclohydrolase IIUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0108.
HOGENOMiHOG000115440.
KOiK14652.
OMAiFDTIERA.
OrthoDBiEOG679TK8.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFiPIRSF001259. RibA. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

C1B4K9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRFDTIERA VADIAAGKAV VVVDDEDREN EGDLIFAAEK ATPELVAFMV
60 70 80 90 100
RYTSGYLCVP LDGADCDRLG LPPMYATNQD KHGTAYTVTV DAREGIGTGI
110 120 130 140 150
SASDRAATMR LLADPSSGAQ DFTRPGHVVP LRAKEGGVLR RPGHTEAAVD
160 170 180 190 200
LARMADLRPA GVICEIVSQK DEGHMAQTDE LRVFADDHDL ALISIADLIA
210 220 230 240 250
WRRKHEKHVE RVASARIPTR HGEFTAVGYR SIYDDVEHVA LVRGDLPGPD
260 270 280 290 300
GDGSDVLVRV HSECLTGDVF GSLRCDCGPQ LDAALDMVAQ EGRGVVLYMR
310 320 330 340 350
GHEGRGIGLM HKLQAYQLQD AGSDTVDANL ELGLPADARD YGIGAQILVD
360 370 380 390 400
LGISSMRLLT NNPAKRVGLD GYGLQITERV SMPLRANAEN LTYLRTKRDR
410
MGHDLIGLDD FEAGEML
Length:417
Mass (Da):45,184
Last modified:May 26, 2009 - v1
Checksum:i96C9BD0ECEC2F875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH55198.1.
RefSeqiWP_015890624.1. NC_012522.1.
YP_002784143.1. NC_012522.1.

Genome annotation databases

EnsemblBacteriaiBAH55198; BAH55198; ROP_69510.
GeneIDi7744531.
KEGGirop:ROP_69510.
PATRICi23231355. VBIRhoOpa21106_7036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH55198.1 .
RefSeqi WP_015890624.1. NC_012522.1.
YP_002784143.1. NC_012522.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 632772.ROP_69510.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH55198 ; BAH55198 ; ROP_69510 .
GeneIDi 7744531.
KEGGi rop:ROP_69510.
PATRICi 23231355. VBIRhoOpa21106_7036.

Phylogenomic databases

eggNOGi COG0108.
HOGENOMi HOG000115440.
KOi K14652.
OMAi FDTIERA.
OrthoDBi EOG679TK8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .
UPA00275 ; UER00400 .
BioCyci ROPA632772:GH0Q-7007-MONOMER.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
HAMAPi MF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001259. RibA. 1 hit.
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B4Imported.

Entry informationi

Entry nameiC1B4K9_RHOOB
AccessioniPrimary (citable) accession number: C1B4K9
Entry historyi
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotation

External Data

Dasty 3