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C1B4K9

- C1B4K9_RHOOB

UniProt

C1B4K9 - C1B4K9_RHOOB

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Protein
Riboflavin biosynthesis protein RibBA
Gene
ribA, ribBA, ROP_69510
Organism
Rhodococcus opacus (strain B4)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.UniRule annotationSAAS annotations
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.UniRule annotationSAAS annotations

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations
Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Magnesium or manganese 1 By similarityUniRule annotation
Metal bindingi29 – 291Magnesium or manganese 2 By similarityUniRule annotation
Binding sitei33 – 331D-ribulose 5-phosphate By similarityUniRule annotation
Sitei127 – 1271Essential for DHBP synthase activity By similarityUniRule annotation
Metal bindingi144 – 1441Magnesium or manganese 2 By similarityUniRule annotation
Binding sitei165 – 1651D-ribulose 5-phosphate By similarityUniRule annotation
Sitei165 – 1651Essential for DHBP synthase activity By similarityUniRule annotation
Metal bindingi264 – 2641Zinc; catalytic By similarityUniRule annotation
Metal bindingi275 – 2751Zinc; catalytic By similarityUniRule annotation
Metal bindingi277 – 2771Zinc; catalytic By similarityUniRule annotation
Binding sitei280 – 2801GTP By similarityUniRule annotation
Binding sitei325 – 3251GTP By similarityUniRule annotation
Active sitei337 – 3371Proton acceptor; for GTP cyclohydrolase activity By similarityUniRule annotation
Active sitei339 – 3391Nucleophile; for GTP cyclohydrolase activity By similarityUniRule annotation
Binding sitei360 – 3601GTP By similarityUniRule annotation
Binding sitei365 – 3651GTP By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi259 – 2635GTP By similarityUniRule annotation
Nucleotide bindingi303 – 3053GTP By similarityUniRule annotation

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
  6. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported, LyaseUniRule annotation

Keywords - Biological processi

Riboflavin biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

GTP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding, ZincUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciROPA632772:GH0Q-7007-MONOMER.
UniPathwayiUPA00275; UER00399.
UPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Gene namesi
Name:ribAImported
Synonyms:ribBAUniRule annotation
Ordered Locus Names:ROP_69510Imported
OrganismiRhodococcus opacus (strain B4)Imported
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000002212: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi632772.ROP_69510.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 204204DHBP synthase By similarityUniRule annotation
Add
BLAST
Regioni28 – 292D-ribulose 5-phosphate binding By similarityUniRule annotation
Regioni141 – 1455D-ribulose 5-phosphate binding By similarityUniRule annotation
Regioni205 – 417213GTP cyclohydrolase II By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0108.
HOGENOMiHOG000115440.
KOiK14652.
OMAiFDTIERA.
OrthoDBiEOG679TK8.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFiPIRSF001259. RibA. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

C1B4K9-1 [UniParc]FASTAAdd to Basket

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MTRFDTIERA VADIAAGKAV VVVDDEDREN EGDLIFAAEK ATPELVAFMV    50
RYTSGYLCVP LDGADCDRLG LPPMYATNQD KHGTAYTVTV DAREGIGTGI 100
SASDRAATMR LLADPSSGAQ DFTRPGHVVP LRAKEGGVLR RPGHTEAAVD 150
LARMADLRPA GVICEIVSQK DEGHMAQTDE LRVFADDHDL ALISIADLIA 200
WRRKHEKHVE RVASARIPTR HGEFTAVGYR SIYDDVEHVA LVRGDLPGPD 250
GDGSDVLVRV HSECLTGDVF GSLRCDCGPQ LDAALDMVAQ EGRGVVLYMR 300
GHEGRGIGLM HKLQAYQLQD AGSDTVDANL ELGLPADARD YGIGAQILVD 350
LGISSMRLLT NNPAKRVGLD GYGLQITERV SMPLRANAEN LTYLRTKRDR 400
MGHDLIGLDD FEAGEML 417
Length:417
Mass (Da):45,184
Last modified:May 26, 2009 - v1
Checksum:i96C9BD0ECEC2F875
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP011115 Genomic DNA. Translation: BAH55198.1.
RefSeqiYP_002784143.1. NC_012522.1.

Genome annotation databases

EnsemblBacteriaiBAH55198; BAH55198; ROP_69510.
GeneIDi7744531.
KEGGirop:ROP_69510.
PATRICi23231355. VBIRhoOpa21106_7036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP011115 Genomic DNA. Translation: BAH55198.1 .
RefSeqi YP_002784143.1. NC_012522.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 632772.ROP_69510.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH55198 ; BAH55198 ; ROP_69510 .
GeneIDi 7744531.
KEGGi rop:ROP_69510.
PATRICi 23231355. VBIRhoOpa21106_7036.

Phylogenomic databases

eggNOGi COG0108.
HOGENOMi HOG000115440.
KOi K14652.
OMAi FDTIERA.
OrthoDBi EOG679TK8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .
UPA00275 ; UER00400 .
BioCyci ROPA632772:GH0Q-7007-MONOMER.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
HAMAPi MF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001259. RibA. 1 hit.
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B4Imported.

Entry informationi

Entry nameiC1B4K9_RHOOB
AccessioniPrimary (citable) accession number: C1B4K9
Entry historyi
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation

External Data

Dasty 3

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