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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribA

Organism
Rhodococcus opacus (strain B4)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibBA (ribA), Riboflavin biosynthesis protein RibBA (ribA)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.SAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. GTP cyclohydrolase-2 (ribA), Riboflavin biosynthesis protein RibBA (ribA), Riboflavin biosynthesis protein RibBA (ribA), GTP cyclohydrolase-2 (ribA)
  2. Riboflavin biosynthesis protein RibD (ribG)
  3. Riboflavin biosynthesis protein RibD (ribG)
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Magnesium or manganese 1UniRule annotation1
Metal bindingi29Magnesium or manganese 2UniRule annotation1
Binding sitei33D-ribulose 5-phosphateUniRule annotation1
Sitei127Essential for DHBP synthase activityUniRule annotation1
Metal bindingi144Magnesium or manganese 2UniRule annotation1
Binding sitei165D-ribulose 5-phosphateUniRule annotation1
Sitei165Essential for DHBP synthase activityUniRule annotation1
Metal bindingi264Zinc; catalyticUniRule annotation1
Metal bindingi275Zinc; catalyticUniRule annotation1
Metal bindingi277Zinc; catalyticUniRule annotation1
Binding sitei280GTPUniRule annotation1
Binding sitei325GTPUniRule annotation1
Active sitei337Proton acceptor; for GTP cyclohydrolase activityUniRule annotation1
Active sitei339Nucleophile; for GTP cyclohydrolase activityUniRule annotation1
Binding sitei360GTPUniRule annotation1
Binding sitei365GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi259 – 263GTPUniRule annotation5
Nucleotide bindingi303 – 305GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported, LyaseUniRule annotation, Multifunctional enzymeUniRule annotation
Biological processRiboflavin biosynthesisUniRule annotationSAAS annotation
LigandGTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00275; UER00399
UPA00275; UER00400

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Including the following 2 domains:
3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation)
Short name:
DHBP synthaseUniRule annotation
GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation)
Alternative name(s):
GTP cyclohydrolase IIUniRule annotation
Gene namesi
Name:ribAImported
Synonyms:ribBAUniRule annotation
Ordered Locus Names:ROP_69510Imported
OrganismiRhodococcus opacus (strain B4)Imported
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000002212 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi632772.ROP_69510

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 380GTP_cyclohydro2InterPro annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 204DHBP synthaseUniRule annotationAdd BLAST204
Regioni28 – 29D-ribulose 5-phosphate bindingUniRule annotation2
Regioni141 – 145D-ribulose 5-phosphate bindingUniRule annotation5
Regioni205 – 417GTP cyclohydrolase IIUniRule annotationAdd BLAST213

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C66 Bacteria
COG0108 LUCA
COG0807 LUCA
HOGENOMiHOG000115440
KOiK14652
OMAiGCTTGIS
OrthoDBiPOG091H008U

Family and domain databases

CDDicd00641 GTP_cyclohydro2, 1 hit
Gene3Di3.40.50.10990, 1 hit
HAMAPiMF_00179 RibA, 1 hit
MF_00180 RibB, 1 hit
MF_01283 RibBA, 1 hit
InterProiView protein in InterPro
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR000422 DHBP_synthase_RibB
IPR032677 GTP_cyclohydro_II
IPR000926 RibA
IPR036144 RibA-like_sf
IPR016299 Riboflavin_synth_RibBA
PfamiView protein in Pfam
PF00926 DHBP_synthase, 1 hit
PF00925 GTP_cyclohydro2, 1 hit
SUPFAMiSSF142695 SSF142695, 1 hit
SSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00505 ribA, 1 hit
TIGR00506 ribB, 1 hit

Sequencei

Sequence statusi: Complete.

C1B4K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRFDTIERA VADIAAGKAV VVVDDEDREN EGDLIFAAEK ATPELVAFMV
60 70 80 90 100
RYTSGYLCVP LDGADCDRLG LPPMYATNQD KHGTAYTVTV DAREGIGTGI
110 120 130 140 150
SASDRAATMR LLADPSSGAQ DFTRPGHVVP LRAKEGGVLR RPGHTEAAVD
160 170 180 190 200
LARMADLRPA GVICEIVSQK DEGHMAQTDE LRVFADDHDL ALISIADLIA
210 220 230 240 250
WRRKHEKHVE RVASARIPTR HGEFTAVGYR SIYDDVEHVA LVRGDLPGPD
260 270 280 290 300
GDGSDVLVRV HSECLTGDVF GSLRCDCGPQ LDAALDMVAQ EGRGVVLYMR
310 320 330 340 350
GHEGRGIGLM HKLQAYQLQD AGSDTVDANL ELGLPADARD YGIGAQILVD
360 370 380 390 400
LGISSMRLLT NNPAKRVGLD GYGLQITERV SMPLRANAEN LTYLRTKRDR
410
MGHDLIGLDD FEAGEML
Length:417
Mass (Da):45,184
Last modified:May 26, 2009 - v1
Checksum:i96C9BD0ECEC2F875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA Translation: BAH55198.1
RefSeqiWP_015890624.1, NC_012522.1

Genome annotation databases

EnsemblBacteriaiBAH55198; BAH55198; ROP_69510
KEGGirop:ROP_69510
PATRICifig|632772.20.peg.7242

Similar proteinsi

Entry informationi

Entry nameiC1B4K9_RHOOB
AccessioniPrimary (citable) accession number: C1B4K9
Entry historyiIntegrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: April 25, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health