C1B4I7 (PYRB_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	ROP_69290

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus › Rhodococcus opacus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP-Rule MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP-Rule MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway 'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Aspartate carbamoyltransferase HAMAP-Rule MF_00001		PRO_1000116154

Sequences

	Sequence		Length	Mass (Da)
	C1B4I7 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 8C2E9EB7D6F857C6	FASTA	314	33,669
	10 20 30 40 50 60 MKHLLSIADL TRESAVELLD EAERFEQALL GREVRKLPTL RGRTVMTVFF ENSTRTRVSF 70 80 90 100 110 120 EVAGKWMSAD VINVSASSSS VSKGESLRDT AMTLRAAGAD ALIVRHPASG AAHQIASWTG 130 140 150 160 170 180 RQDDGGPAVI NAGDGTHEHP TQALLDALTL RQRLGDIEGK RIAIVGDILH SRVARSNALL 190 200 210 220 230 240 LSMLGAEVVL VAPPTLLPVG VSSWPVSVAH SLDAELPGLD AVLMLRVQAE RMNGGFFPSQ 250 260 270 280 290 300 REYSINYGLS EKRLALLPEH AVVLHPGPML RGMEIASAVA DSTRTAVLQQ VTNGVHMRMA 310 VLFRLLVGAE DVAG

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH55176.1.
RefSeq	YP_002784121.1. NC_012522.1.
3D structure databases
ProteinModelPortal	C1B4I7.
ModBase	Search...
Protein-protein interaction databases
STRING	632772.ROP_69290.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAH55176; BAH55176; ROP_69290.
GeneID	7744500.
KEGG	rop:ROP_69290.
PATRIC	23231311. VBIRhoOpa21106_7014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0540.
HOGENOM	HOG000022685.
KO	K00609.
OMA	MTLNAMR.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	ROPA632772:GH0Q-7092-MONOMER.
UniPathway	UPA00070; UER00116.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr.
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR002082. Asp_carbamoyltransf. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_RHOOB

Accession

Primary (citable) accession number: C1B4I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program