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Protein

1-deoxy-D-xylulose-5-phosphate synthase

Gene

dxs

Organism
Rhodococcus opacus (strain B4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).UniRule annotationSAAS annotation

Catalytic activityi

Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891Thiamine pyrophosphateUniRule annotation
Metal bindingi161 – 1611MagnesiumUniRule annotation
Metal bindingi191 – 1911MagnesiumUniRule annotation
Binding sitei191 – 1911Thiamine pyrophosphateUniRule annotation
Binding sitei302 – 3021Thiamine pyrophosphateUniRule annotation
Binding sitei383 – 3831Thiamine pyrophosphateUniRule annotation

GO - Molecular functioni

  1. 1-deoxy-D-xylulose-5-phosphate synthase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. thiamine pyrophosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 1-deoxy-D-xylulose 5-phosphate biosynthetic process Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: UniProtKB-HAMAP
  3. thiamine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Isoprene biosynthesisUniRule annotationSAAS annotation, Thiamine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciROPA632772:GH0Q-6878-MONOMER.
UniPathwayiUPA00064; UER00091.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose-5-phosphate synthaseUniRule annotationSAAS annotation (EC:2.2.1.7UniRule annotationSAAS annotation)
Alternative name(s):
1-deoxyxylulose-5-phosphate synthaseUniRule annotation
Gene namesi
Name:dxsUniRule annotationImported
Ordered Locus Names:ROP_68280Imported
OrganismiRhodococcus opacus (strain B4)Imported
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000002212: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi632772.ROP_68280.

Structurei

3D structure databases

ProteinModelPortaliC1B486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1323Thiamine pyrophosphate bindingUniRule annotation
Regioni162 – 1632Thiamine pyrophosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the transketolase family. DXPS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1154.
HOGENOMiHOG000012986.
KOiK01662.
OMAiCIPNMVV.
OrthoDBiEOG6BKJ6P.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
HAMAPiMF_00315. DXP_synth.
InterProiIPR005477. Dxylulose-5-P_synthase.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR020826. Transketolase_BS.
IPR005476. Transketolase_C.
IPR005474. Transketolase_N.
[Graphical view]
PfamiPF13292. DXP_synthase_N. 1 hit.
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR00204. dxs. 1 hit.
PROSITEiPS00801. TRANSKETOLASE_1. 1 hit.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1B486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEARKAACI TEGAISLGVL ARIQTPDDLR QLNPAEMKQL AAEIREFLVQ
60 70 80 90 100
KVAATGGHLG PNLGVVELTL ALHRIFESPA DPIVFDTGHQ AYVHKILTGR
110 120 130 140 150
KDDFDSLRKQ GGLSGYPCRA ESDHDWVESS HASAALSYAD GLAKAFELTG
160 170 180 190 200
QARHVVAVVG DGALTGGMCW EALNNIAAGK DRSVVIVVND NGRSYAPTIG
210 220 230 240 250
GLADHLAALR LQPGYERILD SGRRMVKKLP WVGRTAYSVL HGMKAGLKDA
260 270 280 290 300
VAPQVMFTDL GIKYLGPVDG HDEAALESAL RRAKAFGGPV IVHAVTRKGM
310 320 330 340 350
GYAPAENHVA DQMHSTGVID PVTGKSAGSA SADWTSVFSA ELIDQASHRQ
360 370 380 390 400
DIVAITAAMA GPTGLAAFGE KYPDRMFDVG IAEQHAVTSA AGLALGGLHP
410 420 430 440 450
VVAVYSTFLN RAFDQLLMDV ALLKLPVTLV LDRAGITGSD GASHNGMWDM
460 470 480 490 500
SLLGIVPGMR VAAPRDTATL REELAEALAV DDGPTALRFP KGTVGDDVPA
510 520 530 540 550
VSRLDGVVDI LHAPSGRNDV LIVSVGAFAG LALAAAERLE QQGISATVVD
560 570 580 590 600
PRWVLPVPES LLKLAEDSTM VVTVEDSGLH GGVGSTVSAA LRAAGVDVPC
610 620 630 640 650
RDLGVPQRFL DHASRAQIHA ELGLTAQDVA RQITGWFAGL GNLRPGQQNG
660
VVADLDAQRA ENRGQ
Length:665
Mass (Da):69,831
Last modified:May 26, 2009 - v1
Checksum:iE0A94A32A2152E0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH55075.1.
RefSeqiYP_002784020.1. NC_012522.1.

Genome annotation databases

EnsemblBacteriaiBAH55075; BAH55075; ROP_68280.
GeneIDi7741621.
KEGGirop:ROP_68280.
PATRICi23231095. VBIRhoOpa21106_6912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH55075.1.
RefSeqiYP_002784020.1. NC_012522.1.

3D structure databases

ProteinModelPortaliC1B486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi632772.ROP_68280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH55075; BAH55075; ROP_68280.
GeneIDi7741621.
KEGGirop:ROP_68280.
PATRICi23231095. VBIRhoOpa21106_6912.

Phylogenomic databases

eggNOGiCOG1154.
HOGENOMiHOG000012986.
KOiK01662.
OMAiCIPNMVV.
OrthoDBiEOG6BKJ6P.

Enzyme and pathway databases

UniPathwayiUPA00064; UER00091.
BioCyciROPA632772:GH0Q-6878-MONOMER.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
HAMAPiMF_00315. DXP_synth.
InterProiIPR005477. Dxylulose-5-P_synthase.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR020826. Transketolase_BS.
IPR005476. Transketolase_C.
IPR005474. Transketolase_N.
[Graphical view]
PfamiPF13292. DXP_synthase_N. 1 hit.
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR00204. dxs. 1 hit.
PROSITEiPS00801. TRANSKETOLASE_1. 1 hit.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B4Imported.

Entry informationi

Entry nameiC1B486_RHOOB
AccessioniPrimary (citable) accession number: C1B486
Entry historyi
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: February 4, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.