C1B472 (C1B472_RHOOB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116 EMBL BAH55061.1
Ordered Locus Names:	ROP_68140

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 SAAS SAAS008181
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 SAAS SAAS008181
Cofactor	Magnesium By similarity. HAMAP MF_00116 SAAS SAAS008181
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116 SAAS SAAS008181
Ligand	Magnesium HAMAP MF_00116 SAAS SAAS008181 Metal-binding HAMAP MF_00116 SAAS SAAS008181
Molecular function	Hydrolase HAMAP MF_00116 SAAS SAAS008181 EMBL BAH55061.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	78 – 80	3	Substrate binding By similarity HAMAP MF_00116
	Region	95 – 97	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	91	1	Substrate By similarity HAMAP MF_00116
	Binding site	105	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	C1B472 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: E920BF78974BE6FA	FASTA	171	17,501
	10 20 30 40 50 60 MSDLSPGHSP AATPTIPPVA LQRLDPGIPV PQRAHDGDAG VDLCSTTDVT IEPGRRVLVG 70 80 90 100 110 120 TGIAVALPLG TVGLIHPRSG LAAKSGLSVV NTPGTIDAGY RGEIKVCLIN HDLETPIEIR 130 140 150 160 170 RGDRIAQLLV QRVELVSFVE VESLDGTTRG SDGHGSSGGH ASLVGNVSEG A

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH55061.1.
RefSeq	YP_002784006.1. NC_012522.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1B472.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7745456.
GenomeReviews	Gene locus ROP_68140 in contig AP011115_GR.
KEGG	rop:ROP_68140.
PATRIC	23231067. VBIRhoOpa21106_6898.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KIAQMVI.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C1B472_RHOOB

Accession

Primary (citable) accession number: C1B472

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)