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C1B324

- PNP_RHOOB

UniProt

C1B324 - PNP_RHOOB

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, ROP_66940
Organism
Rhodococcus opacus (strain B4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi532 – 5321Magnesium By similarity
Metal bindingi538 – 5381Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciROPA632772:GH0Q-6744-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:ROP_66940
OrganismiRhodococcus opacus (strain B4)
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000002212: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000381915Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi632772.ROP_66940.

Structurei

3D structure databases

ProteinModelPortaliC1B324.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini598 – 65760KH
Add
BLAST
Domaini669 – 73870S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiTIAGKQD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1B324-1 [UniParc]FASTAAdd to Basket

« Hide

MTENSAVEVD EGVFESTAVI DNGSFGTRTI RFETGRLAQQ AAGAVVAYLD    50
DETMLLSATS ASKHPKEHFD FFPLTVDVEE RMYAAGRIPG SFFRREGRPS 100
TDAILTCRLI DRPLRPSFVD GLRNEIQVVV TVMSLNPQDL YDVVAINAAS 150
ASTQIAGLPF SGPVGGVRVA LITSDENKAG QWVAFPTVEQ LENAVFDMVV 200
AGRIVSGSGD DADVAIMMVE AEATDNVISL IDGGAQAPTE TIVAEGLEAA 250
KPFIARLCTA QQQLAVKAAK PTGDFPVFPA YQDDVFAAVE AAAAEKLSAA 300
LTIAGKQERD DKTDEVKVEV LEQVAPNFEG REKELGAAFR SLTKKLVRQR 350
ILRDQFRIDG RGITDIRSLS AEVAIVPRAH GSALFERGET QILGVTTLDM 400
VKMAQQVDSL GPETTKRYMH HYNFPPYSTG ETGRVGSPKR REIGHGALAE 450
RALMPVLPSV EEFPYAIRQV SEALSSNGST SMGSVCASTL ALLNAGVPLK 500
APVAGIAMGL VSDQVDGETR YVALTDILGA EDAFGDMDFK VAGTKDFVTA 550
LQLDTKLDGI PSKVLAGALS QAKDARATIL EVMAEAIDTP DEMSPFAPRV 600
TAIKVPVDKI GEVIGPKGKM INSITEQTGA NISIEDDGTV FVGATDGPSA 650
QAAIDMINAI ANPQLPKVGE RFLGTVVKTT AFGAFVSLLP GRDGLVHISK 700
LGSGKRIAKV EDVVSVGSKL RVEIADIDNR GKISLIPVEE DGAAAPAEQS 750
EEAAAEK 757
Length:757
Mass (Da):80,271
Last modified:May 26, 2009 - v1
Checksum:iA16308AE80AA353E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP011115 Genomic DNA. Translation: BAH54941.1.
RefSeqiWP_015890378.1. NC_012522.1.
YP_002783886.1. NC_012522.1.

Genome annotation databases

EnsemblBacteriaiBAH54941; BAH54941; ROP_66940.
GeneIDi7743765.
KEGGirop:ROP_66940.
PATRICi23230821. VBIRhoOpa21106_6775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP011115 Genomic DNA. Translation: BAH54941.1 .
RefSeqi WP_015890378.1. NC_012522.1.
YP_002783886.1. NC_012522.1.

3D structure databases

ProteinModelPortali C1B324.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 632772.ROP_66940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH54941 ; BAH54941 ; ROP_66940 .
GeneIDi 7743765.
KEGGi rop:ROP_66940.
PATRICi 23230821. VBIRhoOpa21106_6775.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi TIAGKQD.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci ROPA632772:GH0Q-6744-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B4.

Entry informationi

Entry nameiPNP_RHOOB
AccessioniPrimary (citable) accession number: C1B324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: May 26, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi