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C1B324

- PNP_RHOOB

UniProt

C1B324 - PNP_RHOOB

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Rhodococcus opacus (strain B4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi532 – 5321MagnesiumUniRule annotation
    Metal bindingi538 – 5381MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciROPA632772:GH0Q-6744-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:ROP_66940
    OrganismiRhodococcus opacus (strain B4)
    Taxonomic identifieri632772 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000002212: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Polyribonucleotide nucleotidyltransferasePRO_0000381915Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi632772.ROP_66940.

    Structurei

    3D structure databases

    ProteinModelPortaliC1B324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini598 – 65760KHUniRule annotationAdd
    BLAST
    Domaini669 – 73870S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiTIAGKQD.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C1B324-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTENSAVEVD EGVFESTAVI DNGSFGTRTI RFETGRLAQQ AAGAVVAYLD    50
    DETMLLSATS ASKHPKEHFD FFPLTVDVEE RMYAAGRIPG SFFRREGRPS 100
    TDAILTCRLI DRPLRPSFVD GLRNEIQVVV TVMSLNPQDL YDVVAINAAS 150
    ASTQIAGLPF SGPVGGVRVA LITSDENKAG QWVAFPTVEQ LENAVFDMVV 200
    AGRIVSGSGD DADVAIMMVE AEATDNVISL IDGGAQAPTE TIVAEGLEAA 250
    KPFIARLCTA QQQLAVKAAK PTGDFPVFPA YQDDVFAAVE AAAAEKLSAA 300
    LTIAGKQERD DKTDEVKVEV LEQVAPNFEG REKELGAAFR SLTKKLVRQR 350
    ILRDQFRIDG RGITDIRSLS AEVAIVPRAH GSALFERGET QILGVTTLDM 400
    VKMAQQVDSL GPETTKRYMH HYNFPPYSTG ETGRVGSPKR REIGHGALAE 450
    RALMPVLPSV EEFPYAIRQV SEALSSNGST SMGSVCASTL ALLNAGVPLK 500
    APVAGIAMGL VSDQVDGETR YVALTDILGA EDAFGDMDFK VAGTKDFVTA 550
    LQLDTKLDGI PSKVLAGALS QAKDARATIL EVMAEAIDTP DEMSPFAPRV 600
    TAIKVPVDKI GEVIGPKGKM INSITEQTGA NISIEDDGTV FVGATDGPSA 650
    QAAIDMINAI ANPQLPKVGE RFLGTVVKTT AFGAFVSLLP GRDGLVHISK 700
    LGSGKRIAKV EDVVSVGSKL RVEIADIDNR GKISLIPVEE DGAAAPAEQS 750
    EEAAAEK 757
    Length:757
    Mass (Da):80,271
    Last modified:May 26, 2009 - v1
    Checksum:iA16308AE80AA353E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP011115 Genomic DNA. Translation: BAH54941.1.
    RefSeqiWP_015890378.1. NC_012522.1.
    YP_002783886.1. NC_012522.1.

    Genome annotation databases

    EnsemblBacteriaiBAH54941; BAH54941; ROP_66940.
    GeneIDi7743765.
    KEGGirop:ROP_66940.
    PATRICi23230821. VBIRhoOpa21106_6775.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP011115 Genomic DNA. Translation: BAH54941.1 .
    RefSeqi WP_015890378.1. NC_012522.1.
    YP_002783886.1. NC_012522.1.

    3D structure databases

    ProteinModelPortali C1B324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 632772.ROP_66940.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH54941 ; BAH54941 ; ROP_66940 .
    GeneIDi 7743765.
    KEGGi rop:ROP_66940.
    PATRICi 23230821. VBIRhoOpa21106_6775.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi TIAGKQD.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci ROPA632772:GH0Q-6744-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
      Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
      Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B4.

    Entry informationi

    Entry nameiPNP_RHOOB
    AccessioniPrimary (citable) accession number: C1B324
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3