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C1B313 (IF2_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:ROP_66830
OrganismRhodococcus opacus (strain B4) [Complete proteome] [HAMAP]
Taxonomic identifier632772 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length974 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 974974Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000118771

Regions

Nucleotide binding479 – 4868GTP By similarity
Nucleotide binding529 – 5335GTP By similarity
Nucleotide binding583 – 5864GTP By similarity
Region473 – 625153G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
C1B313 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 82E392A60C241398

FASTA974100,063
        10         20         30         40         50         60 
MAGKARVHEL AKELGVTSKE LLATLKEQGE FVKSASSTVE APVARRLRES FPSAKSADSA 

        70         80         90        100        110        120 
ARPAAKPGAP APSTPATSAK PGGPRPGPKP AAPAPAAPAA AAPAATPAAQ APAPAAPAAS 

       130        140        150        160        170        180 
TATPAAPASN APKPGRPTPA APAPAAPAAP AAPAAASTPA APSTGAKPGG PRPGPKPPRV 

       190        200        210        220        230        240 
GNNPYSSAPA ERPAPRPAPG APRPGAPRPA PGQGGPRPAP GQGGPRPAPG QGGPRPAPGQ 

       250        260        270        280        290        300 
GGPRPAPGQG GPRPSPGSMP PRPNPGAMPA RSARPAPGGR PGRPGGAPGG RPGGGGGGYR 

       310        320        330        340        350        360 
GGGAPGAGAG APGGGAPAGG FRGRPGGGGR PGQRGAAAGA FGRPGGAPRR GRKSKRQKRQ 

       370        380        390        400        410        420 
EYDSMQAPAV GGVRLPRGNG ETIRLARGAS LSDFAEKIDA NPAALVQALF NLGEMVTATQ 

       430        440        450        460        470        480 
SVNDETLELL GGEMNYVVQV VSPEDEDREL LDSFDLTYGE DEGGEEDLES RPPVVTVMGH 

       490        500        510        520        530        540 
VDHGKTRLLD TIRKANVREG EAGGITQHIG AYQVLTELDG NERLVTFIDT PGHEAFTAMR 

       550        560        570        580        590        600 
ARGAKATDLA ILVVAADDGV MPQTVEAINH AQAADVPIVV AVNKIDKEGA NPDKIRQQLT 

       610        620        630        640        650        660 
EYGLVAEEYG GDTMFVDISA KQGTNIDALL EAVLLTADAA LDLRANPDMD AQGVAIEAHL 

       670        680        690        700        710        720 
DRGRGPVATV LIQRGTLRVG DSIVAGDAYG RVRRMVDEHG DDVLEAMPSR PVQVVGFTSV 

       730        740        750        760        770        780 
PGAGDNLLVV DEDRIARQIA DRRNARKRNA LAAKSRKRIS LEDLDSALKE TSQLNLILKG 

       790        800        810        820        830        840 
DNSGTVEALE EALHGIEIDD EVQLRVIDRG VGGVTETNVN LAAASNAIII GFNVRAEGKA 

       850        860        870        880        890        900 
TELANREGVD IRYYSVIYQA IDEVEKALKG MLKPIYEEVE LGKAEIRAMF RSSKVGNIAG 

       910        920        930        940        950        960 
CLVTSGTIRR NAKARLLRDN TVVAETVTIS SLKREKEDAV EVREGYECGL TLTYSDIKVG 

       970 
DVIEAYELRE KPRD 

« Hide

References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP011115 Genomic DNA. Translation: BAH54930.1.
RefSeqYP_002783875.1. NC_012522.1.

3D structure databases

ProteinModelPortalC1B313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING632772.ROP_66830.

Proteomic databases

PRIDEC1B313.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH54930; BAH54930; ROP_66830.
GeneID7745365.
KEGGrop:ROP_66830.
PATRIC23230799. VBIRhoOpa21106_6764.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
KOK02519.
OMANPDMDAQ.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycROPA632772:GH0Q-6733-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIF2_RHOOB
AccessionPrimary (citable) accession number: C1B313
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries