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C1B2N8 (DDL_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:ROP_65580
OrganismRhodococcus opacus (strain B4) [Complete proteome] [HAMAP]
Taxonomic identifier632772 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000189743

Regions

Domain151 – 358208ATP-grasp
Nucleotide binding179 – 23456ATP By similarity

Sites

Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3251Magnesium or manganese 1 By similarity
Metal binding3251Magnesium or manganese 2 By similarity
Metal binding3271Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
C1B2N8 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 8444A75BCA579AF0

FASTA36839,036
        10         20         30         40         50         60 
MSKPRTRVAV IFGGRSNEHS VSCVSAGSVL RNLDPERYEV VPIGITTEGS WVLGSTDPES 

        70         80         90        100        110        120 
LSIRGRSLPS VDADGSALAL TADPTRSGDL VALDDGEAGK ILASVDVVFP VLHGAYGEDG 

       130        140        150        160        170        180 
TIQGLLELAG VPYVGPGVLA SAAGMDKEFT KKLLAAEGLP IGFQVVLRPG TATLTDEQRS 

       190        200        210        220        230        240 
RLHLPVFVKP ARGGSSIGIT RVAEWAALDD AIAHARRHDP KVIVESGIAG REVECGVLEF 

       250        260        270        280        290        300 
PDGDVRASVI AEIRMPEGAD DDEAFYDFDS KYLDDVCEFD VPAKLEDSVS DEIRALAVRA 

       310        320        330        340        350        360 
FSALDCQGLA RVDFFVTEDG PVINEINTMP GFTSISMYPR MWDAVGVDYG TLVSTLVDTA 


LARGTGLR 

« Hide

References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP011115 Genomic DNA. Translation: BAH54805.1.
RefSeqYP_002783750.1. NC_012522.1.

3D structure databases

ProteinModelPortalC1B2N8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING632772.ROP_65580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH54805; BAH54805; ROP_65580.
GeneID7745281.
KEGGrop:ROP_65580.
PATRIC23230549. VBIRhoOpa21106_6639.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycROPA632772:GH0Q-6608-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RHOOB
AccessionPrimary (citable) accession number: C1B2N8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways