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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Rhodococcus opacus (strain B4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191D-inositol 3-phosphateUniRule annotation1
Binding sitei33UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei881D-inositol 3-phosphateUniRule annotation1
Binding sitei1211D-inositol 3-phosphateUniRule annotation1
Binding sitei1451D-inositol 3-phosphateUniRule annotation1
Binding sitei1651D-inositol 3-phosphateUniRule annotation1
Binding sitei239UDP-GlcNAcUniRule annotation1
Binding sitei244UDP-GlcNAcUniRule annotation1
Binding sitei303UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi312Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi313Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi315Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei325UDP-GlcNAcUniRule annotation1
Binding sitei333UDP-GlcNAcUniRule annotation1
Metal bindingi339MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:ROP_17450
OrganismiRhodococcus opacus (strain B4)
Taxonomic identifieri632772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000002212 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001511 – 446D-inositol 3-phosphate glycosyltransferaseAdd BLAST446

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi632772.ROP_17450.

Structurei

3D structure databases

ProteinModelPortaliC1AZ64.
SMRiC1AZ64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 26UDP-GlcNAc bindingUniRule annotation2
Regioni30 – 351D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

C1AZ64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTPLHNSRP RRVAVLSVHT SPLAQPGTGD AGGMNVYVLQ SAIQMARRGV
60 70 80 90 100
EVEIFTRATS SADAPVQEAA PGVLVRNVVA GPFEGLDKQD LPTQLCAFVA
110 120 130 140 150
GVLREEARHE PGYYNLVHSH YWLSGQVGWL ARDRWGVPLV HTAHTLAAVK
160 170 180 190 200
NLSLAEGDTP EPAARQIGEQ QVVAESDRLV ANTTEESDQL VRHYGADPNR
210 220 230 240 250
IDVVAPGADL TRYRPGDRDA ARATLGLDPR ETVVTFVGRI QPLKAPDVLL
260 270 280 290 300
RAAAELIARD PASSLRVLVV GGPSGSGLAR PDALIELASS LGIAARVTFL
310 320 330 340 350
PPQAPDRLAD VYRASDLVAV PSYSESFGLV AIEAQACGTP VIAANVGGLG
360 370 380 390 400
VAVRSGETGL LVDGHRTEDW ATALQSLVSE PARLAALAAE APRHAENFSW
410 420 430 440
EHTADGLLES YRMATVNYNY GHGPSEFSPR RGRGLWKLRR AGGVRA
Length:446
Mass (Da):47,503
Last modified:May 26, 2009 - v1
Checksum:i38242ADB0D5DFA7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH49992.1.

Genome annotation databases

EnsemblBacteriaiBAH49992; BAH49992; ROP_17450.
KEGGirop:ROP_17450.
PATRICi23220719. VBIRhoOpa21106_1754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011115 Genomic DNA. Translation: BAH49992.1.

3D structure databases

ProteinModelPortaliC1AZ64.
SMRiC1AZ64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi632772.ROP_17450.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH49992; BAH49992; ROP_17450.
KEGGirop:ROP_17450.
PATRICi23220719. VBIRhoOpa21106_1754.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_RHOOB
AccessioniPrimary (citable) accession number: C1AZ64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 26, 2009
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.