C1AYI4 (ISPH_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	ROP_59320

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000124291

Sequences

	Sequence		Length	Mass (Da)
	C1AYI4 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 6B9650B74BCF3E03	FASTA	336	36,234
	10 20 30 40 50 60 MSSAVPLNVG ITRSADSGAS RADGEKRVLL AEPRGYCAGV DRAVETVEKA LEKHGAPIYV 70 80 90 100 110 120 RKEIVHNRHV VETLSDQGVV FVDETDEVPE GALLVFSAHG VSPAVHESAA ARNLRTIDAT 130 140 150 160 170 180 CPLVTKVHQE AKRFARDDFD ILLIGHEGHE EVEGTAGEAP DHVQLVDGPD SVDAVTVRDE 190 200 210 220 230 240 SKVIWLSQTT LSVDETMQTV ARLRERFPSL QDPPSDDICY ATQNRQVAVK AMAPECDLVI 250 260 270 280 290 300 VVGSRNSSNS VRLVEVALGA GAKASYLVDY AREVDPAWLD GVRTVGITSG ASVPEILVRG 310 320 330 VIDLLDEHGF HDVQPVTTAN ETLVFALPRE LRAART

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH54179.1.
RefSeq	YP_002783124.1. NC_012522.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1AYI4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7746550.
GenomeReviews	Gene locus ROP_59320 in contig AP011115_GR.
KEGG	rop:ROP_59320.
PATRIC	23229245. VBIRhoOpa21106_5991.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AMAPECD.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_RHOOB

Accession

Primary (citable) accession number: C1AYI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program