C1AVX5 (C1AVX5_RHOOB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL BAH49405.1
Ordered Locus Names:	ROP_11580

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 EMBL BAH49405.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	408	1	Nucleophile By similarity HAMAP MF_00685
	Active site	461	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	C1AVX5 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 19F42F8771D524E0	FASTA	732	81,652
	10 20 30 40 50 60 MTVEPPVAIA PDAADLDLLA RGEHHDPHSI LGAHPHPEGT VIRALRPYAE AVDAVIGGTS 70 80 90 100 110 120 FSLEHLAHGV WGALVPYRDL MDYRLSTTWP GGHNDVSADG YRFLPTLGEL DLHLFGEGRH 130 140 150 160 170 180 ERLWEILGAH RRRYDTPDGT VTGTSFAVWA PNARGVSVIG DFDGWSGRNF PMRVLGSTGV 190 200 210 220 230 240 WELFVPGIEV GDLYKFRVHG RDGSVRDKAD PMAFATEVPP ATASRVSVST FEWNDADWLA 250 260 270 280 290 300 QRAATEPAQS PMSVYEVHLG SWRPGLNYRE MAEQLAAHVT ETGFTHVELL PVAEHPFGGS 310 320 330 340 350 360 WGYQVTSYYA PTSRFGSPDD FRWFVDHLHA AGIGVIVDWV PAHFPKDEWA LARFDGTPLY 370 380 390 400 410 420 EHSDPQRGEQ LDWGTYVFDF GRREVRNFLV ANALYWLDEF HVDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 PEGGWTPNIH GGRENLEAVA FLQETNATVH KQHRGVVTIA EESTAWPGVT RATNVGGLGF 490 500 510 520 530 540 NMKWNMGWMH DTLGFLAHDP VHRSYHHHEI TFSLMYAWSE NYLLPISHDE VVHGKGTLWT 550 560 570 580 590 600 RMPGDDWAKA AGVRALLAYM WSHPGKQLLF MGQEFGQTAE WSEERGLDWY QLDDPYTGGF 610 620 630 640 650 660 HRGLLRLVHD LNATYREHPA LWTLDTSPGG FSWIDANDTA NNVLSFLRYG TDGSILACLF 670 680 690 700 710 720 NFSGSPHANY RVGLPERGEW REILNTDAEV YAGSGWGNLG AVTATSQPWH GRPASAEVAL 730 PANGAIWMSL ER

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH49405.1.
RefSeq	YP_002778350.1. NC_012522.1.
3D structure databases
ProteinModelPortal	C1AVX5.
ModBase	Search...
Protein-protein interaction databases
STRING	C1AVX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7741599.
GenomeReviews	Gene locus ROP_11580 in contig AP011115_GR.
KEGG	rop:ROP_11580.
PATRIC	23219513. VBIRhoOpa21106_1160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C1AVX5_RHOOB

Accession

Primary (citable) accession number: C1AVX5

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)