C1AVJ9 (PROB_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	ROP_10320

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Glutamate 5-kinase HAMAP MF_00456		PRO_1000193702
Regions
	Domain	278 – 352	75	PUA

Sequences

	Sequence		Length	Mass (Da)
	C1AVJ9 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 2779027C82A8F8B8	FASTA	366	37,538
	10 20 30 40 50 60 MSATRRTVAS AGSIVVKIGS SALTSLVGGL DVGRLDALAD AIEARMRAGS DVVVVSSGAV 70 80 90 100 110 120 GAGLAPLGLT KRPRDLATKQ AAASVGQLAL AHAWGTSFAR YGRTVGQVLL TADDIARRAQ 130 140 150 160 170 180 HRNAQRTLDR LRALHAVAIV NENDTVATAE LRFGDNDRLA ALVAHLVGAD ALVLLSDVDG 190 200 210 220 230 240 LYDGDPRKGN ATLIPEVNSP EDLDGVVAGS GGALGTGGMA SKLSAARLAA DAGVPVLLAA 250 260 270 280 290 300 ASDAGAALRD ASVGTAFAAR PSRLSARKFW VRHAADEQGI LHIDEGAVRA VVTRRRSLLP 310 320 330 340 350 360 AGITAVSGRF YGGDVVSLLG PEERPVARGV VAYDSAEISD ILGKSTQELP AEMQRPVVHA DDLVPL

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH49279.1.
RefSeq	YP_002778224.1. NC_012522.1.
3D structure databases
ProteinModelPortal	C1AVJ9.
ModBase	Search...
Protein-protein interaction databases
STRING	C1AVJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7744133.
GenomeReviews	Gene locus ROP_10320 in contig AP011115_GR.
KEGG	rop:ROP_10320.
PATRIC	23219251. VBIRhoOpa21106_1035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_RHOOB

Accession

Primary (citable) accession number: C1AVJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program