C1AU63 (RSMH_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	ROP_08240

Organism

Rhodococcus opacus (strain B4) [Complete proteome] [HAMAP]

Taxonomic identifier

632772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387077
Regions
	Region	53 – 55	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity
	Binding site	130	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1AU63 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 919057F1644873D5	FASTA	338	36,629
	10 20 30 40 50 60 MVDHEGEDSS PADGFGHIPV LLHRADELLG PALTANDPHG GGAVMIDATL GLGGHSEHFL 70 80 90 100 110 120 RTYPQLRLVA LDRDPHALEI AGGRLAPFAD RITFVHTRYD GIEDALTQAG LSPRESVHGI 130 140 150 160 170 180 LFDLGVSSMQ LDESDRGFAY SIDAPLDMRM DPTTGITAAE VLNTYSHGDL ARILSTYGEE 190 200 210 220 230 240 RFAGKIASEV VRQRAKEPFT TSAALVELLY RTIPAATRRT GGHPAKRTFQ ALRVEVNGEL 250 260 270 280 290 300 DSLRAAVPAA LDALTVGGRV VFMSYQSLED RVVKQEITPR SKSKSPEGLP VELPGMGPEF 310 320 330 RILTRGAERA SEQEIEENPR SAPVRLRAAE RIARRSAA

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011115 Genomic DNA. Translation: BAH49071.1.
RefSeq	YP_002778016.1. NC_012522.1.
3D structure databases
ProteinModelPortal	C1AU63.
ModBase	Search...
Protein-protein interaction databases
STRING	C1AU63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7747894.
GenomeReviews	Gene locus ROP_08240 in contig AP011115_GR.
KEGG	rop:ROP_08240.
PATRIC	23218821. VBIRhoOpa21106_0823.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NPATRAF.
ProtClustDB	PRK00050.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_RHOOB

Accession

Primary (citable) accession number: C1AU63

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program