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C1ASS8 (MSHC_RHOOB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Short name=L-Cys:GlcN-Ins ligase
EC=6.3.1.13
Alternative name(s):
Mycothiol ligase
Short name=MSH ligase
Gene names
Name:mshC
Ordered Locus Names:ROP_06130
OrganismRhodococcus opacus (strain B4) [Complete proteome] [HAMAP]
Taxonomic identifier632772 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus ›

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins By similarity. HAMAP-Rule MF_01697

Catalytic activity

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate. HAMAP-Rule MF_01697

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01697

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01697

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase HAMAP-Rule MF_01697
PRO_0000400476

Regions

Region43 – 464Cysteinyl adenylate binding By similarity
Region81 – 833Cysteinyl adenylate binding By similarity
Region249 – 2513Cysteinyl adenylate binding By similarity
Motif45 – 5511"HIGH" region By similarity
Motif187 – 1926"ERGGDP" region By similarity
Motif289 – 2935"KMSKS" region By similarity

Sites

Metal binding431Zinc By similarity
Metal binding2311Zinc By similarity
Metal binding2561Zinc By similarity
Binding site581Cysteinyl adenylate By similarity
Binding site2271Cysteinyl adenylate By similarity
Binding site2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
C1ASS8 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 99C2B1F764035985

FASTA41545,710
        10         20         30         40         50         60 
MQSWSETAVP SVPGQGPPLR LFDTADRQVR PVTPGRTATM YVCGITPYDA THLGHAATYL 

        70         80         90        100        110        120 
TFDLVNRIWR DAGHDVHYVQ NVTDVDDPLF ERANRDGEDW VVLGMRETAL FREDMEALRV 

       130        140        150        160        170        180 
LPPRDYIGAV ESIGEVIEMV EKFVASGAAY VVDDPEFPDV YFRADATEQF GYESGYDRAT 

       190        200        210        220        230        240 
MDTFFAERGG DPDRPGKQNP LDALVWRAAR PGEPSWPSPF GPGRPGWHIE CSAIALNRIG 

       250        260        270        280        290        300 
SGFDVQGGGS DLIFPHHEYS AAHAESATGD RRFARHYVHT GMIGLDGEKM SKSRGNLVFV 

       310        320        330        340        350        360 
SKLRGEGVDP AAIRLGLLSG HYRQDRPWTE QVLADAHTRL QLWKDAAALE SAQSATDTIA 

       370        380        390        400        410 
RLRQHLADDL DTPKALDALD GWARRALDNG GSDTNAPTEF AAAVDALLGV RLRRP

« Hide

References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP011115 Genomic DNA. Translation: BAH48860.1.
RefSeqYP_002777805.1. NC_012522.1.

3D structure databases

ProteinModelPortalC1ASS8.
ModBaseSearch...

Protein-protein interaction databases

STRING632772.ROP_06130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH48860; BAH48860; ROP_06130.
GeneID7747768.
KEGGrop:ROP_06130.
PATRIC23218379. VBIRhoOpa21106_0608.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0215.
HOGENOMHOG000245251.
KOK15526.
OMADVHYVQN.
ProtClustDBPRK12418.

Enzyme and pathway databases

BioCycROPA632772:GH0Q-612-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_01697. MshC.
InterProIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
TIGRFAMsTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMSHC_RHOOB
AccessionPrimary (citable) accession number: C1ASS8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 26, 2009
Last modified: May 29, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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