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Protein

Mycothiol acetyltransferase

Gene

mshD

Organism
Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.UniRule annotation

Catalytic activityi

Desacetylmycothiol + acetyl-CoA = CoA + mycothiol.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Desacetylmycothiol; via amide nitrogenUniRule annotation
Binding sitei179 – 1791DesacetylmycothiolUniRule annotation
Binding sitei224 – 2241DesacetylmycothiolUniRule annotation
Binding sitei234 – 2341DesacetylmycothiolUniRule annotation
Binding sitei282 – 2821Desacetylmycothiol; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMBOV561275:GHDN-849-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mycothiol acetyltransferaseUniRule annotation (EC:2.3.1.189UniRule annotation)
Short name:
MSH acetyltransferaseUniRule annotation
Alternative name(s):
Mycothiol synthaseUniRule annotation
Gene namesi
Name:mshDUniRule annotation
Ordered Locus Names:JTY_0841
OrganismiMycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)
Taxonomic identifieri561275 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Mycothiol acetyltransferasePRO_0000400270Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC1ALF4.
SMRiC1ALF4. Positions 5-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 141138N-acetyltransferase 1UniRule annotationAdd
BLAST
Domaini152 – 315164N-acetyltransferase 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Acetyl-CoA binding 1UniRule annotation
Regioni88 – 936Acetyl-CoA binding 1UniRule annotation
Regioni238 – 2403Acetyl-CoA binding 2UniRule annotation
Regioni245 – 2517Acetyl-CoA binding 2UniRule annotation
Regioni287 – 2926Acetyl-CoA binding 2UniRule annotation

Sequence similaritiesi

Belongs to the acetyltransferase family. MshD subfamily.UniRule annotation
Contains 2 N-acetyltransferase domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000248937.
KOiK15520.
OMAiMLYVDES.
OrthoDBiEOG69D3BP.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_01698. MshD.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR017813. Mycothiol_AcTrfase.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 2 hits.
[Graphical view]
PIRSFiPIRSF021524. MSH_acetyltransferase. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03448. mycothiol_MshD. 1 hit.
PROSITEiPS51186. GNAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1ALF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALDWRSAL TADEQRSVRA LVTATTAVDG VAPVGEQVLR ELGQQRTEHL
60 70 80 90 100
LVAGSRPGGP IIGYLNLSPP RGAGGAMAEL VVHPQSRRRG IGTAMARAAL
110 120 130 140 150
AKTAGRNQFW AHGTLDPARA TASALGLVGV RELIQMRRPL RDIPEPTIPD
160 170 180 190 200
GVVIRTYAGT SDDAELLRVN NAAFAGHPEQ GGWTAVQLAE RRGEAWFDPD
210 220 230 240 250
GLILAFGDSP RERPGRLLGF HWTKVHPDHP GLGEVYVLGV DPAAQRRGLG
260 270 280 290 300
QMLTSIGIVS LARRLGGRKT LDPAVEPAVL LYVESDNVAA VRTYQSLGFT
310
TYSVDTAYAL AGTDN
Length:315
Mass (Da):33,599
Last modified:May 26, 2009 - v1
Checksum:iE1728C0652CF2716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010918 Genomic DNA. Translation: BAH25133.1.
RefSeqiWP_003404307.1. NZ_CP014566.1.

Genome annotation databases

EnsemblBacteriaiBAH25133; BAH25133; JTY_0841.
KEGGimbt:JTY_0841.
PATRICi18021047. VBIMycBov85238_0917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010918 Genomic DNA. Translation: BAH25133.1.
RefSeqiWP_003404307.1. NZ_CP014566.1.

3D structure databases

ProteinModelPortaliC1ALF4.
SMRiC1ALF4. Positions 5-311.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH25133; BAH25133; JTY_0841.
KEGGimbt:JTY_0841.
PATRICi18021047. VBIMycBov85238_0917.

Phylogenomic databases

HOGENOMiHOG000248937.
KOiK15520.
OMAiMLYVDES.
OrthoDBiEOG69D3BP.

Enzyme and pathway databases

BioCyciMBOV561275:GHDN-849-MONOMER.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_01698. MshD.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR017813. Mycothiol_AcTrfase.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 2 hits.
[Graphical view]
PIRSFiPIRSF021524. MSH_acetyltransferase. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03448. mycothiol_MshD. 1 hit.
PROSITEiPS51186. GNAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette-Guerin (BCG) Tokyo 172: a comparative study of BCG vaccine substrains."
    Seki M., Honda I., Fujita I., Yano I., Yamamoto S., Koyama A.
    Vaccine 27:1710-1716(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCG / Tokyo 172 / ATCC 35737 / TMC 1019.

Entry informationi

Entry nameiMSHD_MYCBT
AccessioniPrimary (citable) accession number: C1ALF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 26, 2009
Last modified: July 6, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.