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C1AFI5

- DAPF_MYCBT

UniProt

C1AFI5 - DAPF_MYCBT

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Protein
Diaminopimelate epimerase
Gene
dapF, JTY_2733
Organism
Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Substrate By similarity
Binding sitei44 – 441Substrate By similarity
Binding sitei78 – 781Substrate By similarity
Active sitei87 – 871Proton donor/acceptor By similarity
Binding sitei163 – 1631Substrate By similarity
Sitei165 – 1651Important for catalytic activity By similarity
Binding sitei199 – 1991Substrate By similarity
Sitei217 – 2171Important for catalytic activity By similarity
Active sitei226 – 2261Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMBOV561275:GHDN-2767-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:JTY_2733
OrganismiMycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)
Taxonomic identifieri561275 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000002205: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Diaminopimelate epimeraseUniRule annotation
PRO_1000124427Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 226UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi561275.JTY_2733.

Structurei

3D structure databases

ProteinModelPortaliC1AFI5.
SMRiC1AFI5. Positions 1-289.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Substrate binding By similarity
Regioni217 – 2182Substrate binding By similarity
Regioni227 – 2282Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220467.
KOiK01778.
OMAiITIEMGT.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1AFI5-1 [UniParc]FASTAAdd to Basket

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MIFAKGHGTQ NDFVLLPDVD AELVLTAARV AALCDRRKGL GADGVLRVTT    50
AGAAQAVGVL DSLPEGVRVT DWYMDYRNAD GSAAQMCGNG VRVFAHYLRA 100
SGLEVRDEFV VGSLAGPRPV TCHHVEAAYA DVSVDMGKAN RLGAGEAVVG 150
GRRFHGLAVD VGNPHLACVD SQLTVDGLAA LDVGAPVSFD GAQFPDGVNV 200
EVLTAPVDGA VWMRVHERGV GETRSCGTGT VAAAVAALAA VGSPTGTLTV 250
HVPGGEVVVT VTDATSFLRG PSVLVARGDL ADDWWNAMG 289
Length:289
Mass (Da):29,698
Last modified:May 26, 2009 - v1
Checksum:i197FE4B43CD8A022
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010918 Genomic DNA. Translation: BAH27014.1.
RefSeqiYP_002645782.1. NC_012207.1.

Genome annotation databases

EnsemblBacteriaiBAH27014; BAH27014; JTY_2733.
GeneIDi7563307.
KEGGimbt:JTY_2733.
PATRICi18025229. VBIMycBov85238_2979.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010918 Genomic DNA. Translation: BAH27014.1 .
RefSeqi YP_002645782.1. NC_012207.1.

3D structure databases

ProteinModelPortali C1AFI5.
SMRi C1AFI5. Positions 1-289.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561275.JTY_2733.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH27014 ; BAH27014 ; JTY_2733 .
GeneIDi 7563307.
KEGGi mbt:JTY_2733.
PATRICi 18025229. VBIMycBov85238_2979.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220467.
KOi K01778.
OMAi ITIEMGT.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci MBOV561275:GHDN-2767-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette-Guerin (BCG) Tokyo 172: a comparative study of BCG vaccine substrains."
    Seki M., Honda I., Fujita I., Yano I., Yamamoto S., Koyama A.
    Vaccine 27:1710-1716(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCG / Tokyo 172 / ATCC 35737 / TMC 1019.

Entry informationi

Entry nameiDAPF_MYCBT
AccessioniPrimary (citable) accession number: C1AFI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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