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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi115 – 1151Magnesium; catalyticUniRule annotation
Sitei116 – 1161Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei139 – 1391Proton acceptorUniRule annotation
Binding sitei174 – 1741Substrate; shared with dimeric partnerUniRule annotation
Binding sitei234 – 2341SubstrateUniRule annotation
Binding sitei277 – 2771Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATPUniRule annotation
Nucleotide bindingi114 – 1174ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGAUR379066:GI3W-2821-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotationSAAS annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:GAU_2768Imported
OrganismiGemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505)Imported
Taxonomic identifieri379066 [NCBI]
Taxonomic lineageiBacteriaGemmatimonadetesGemmatimonadalesGemmatimonadaceaeGemmatimonas
ProteomesiUP000002209 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi379066.GAU_2768.

Structurei

3D structure databases

ProteinModelPortaliC1AAL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1393Substrate bindingUniRule annotation
Regioni181 – 1833Substrate bindingUniRule annotation
Regioni283 – 2864Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiMHYETQV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1AAL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIAISTGGG DAPGLNAVIR AAVLSARTRG WDVLGIKRGF AGLLGEDEIV
60 70 80 90 100
PLTADTVRGI AGQGGTIIKT TNRGSPFAYP VQAPDGTWST VDRSDELVEN
110 120 130 140 150
ARNLGIEAII SIGGDGSLAI AQKLVAKGVR VVSVPKTIDN DVAGTITTFG
160 170 180 190 200
FDTAVNTAME AIDKLHTTAE SHDRVMVLEV MGREAGFIAL HAGVAGTADV
210 220 230 240 250
ILIPEIEWDL DKVCEKIMAR DATGRRFSIV VVAEGAKPRG GQESIIGESL
260 270 280 290 300
PGQDRRLGGI AERLGFDIQR KTGKETRAMV LGHLQRGGGP TGYDRLLATR
310 320 330 340 350
FGAAAVQAVA DKKWGHMVAL QSPHLVTIPI EQVLAETKRV DPSHDVVQAA

RMMGISFGD
Length:359
Mass (Da):37,853
Last modified:May 26, 2009 - v1
Checksum:iACE92D50D15B2949
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009153 Genomic DNA. Translation: BAH39810.1.
RefSeqiWP_015894579.1. NC_012489.1.
YP_002762280.1. NC_012489.1.

Genome annotation databases

EnsemblBacteriaiBAH39810; BAH39810; GAU_2768.
KEGGigau:GAU_2768.
PATRICi21958528. VBIGemAur55831_2840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009153 Genomic DNA. Translation: BAH39810.1.
RefSeqiWP_015894579.1. NC_012489.1.
YP_002762280.1. NC_012489.1.

3D structure databases

ProteinModelPortaliC1AAL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi379066.GAU_2768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH39810; BAH39810; GAU_2768.
KEGGigau:GAU_2768.
PATRICi21958528. VBIGemAur55831_2840.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiMHYETQV.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciGAUR379066:GI3W-2821-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Gemmatimonas aurantiaca T-27 that represents a novel phylum Gemmatimonadetes."
    Takasaki K., Ichikawa N., Miura H., Matsushita S., Watanabe Y., Oguchi A., Ankai A., Yashiro I., Takahashi M., Terui Y., Fukui S., Yokoyama H., Tanikawa S., Hanada S., Kamagata Y., Fujita N.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T-27 / DSM 14586 / JCM 11422 / NBRC 100505Imported.

Entry informationi

Entry nameiC1AAL2_GEMAT
AccessioniPrimary (citable) accession number: C1AAL2
Entry historyi
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: April 29, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.