C1A375 (C1A375_RHOE4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061 EMBL BAH35060.1
Ordered Locus Names:	RER_43520

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL BAH35060.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	121 – 131	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	36	1	By similarity HAMAP MF_00061
	Active site	163	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	C1A375 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 39DF539B87E19373	FASTA	331	34,093
	10 20 30 40 50 60 MGCGGRRFSP RCSQRLLLFL VLSVVPRPVV VRAPSKVNLH LAVGDLRDDG YHELTTVFQA 70 80 90 100 110 120 LSLADTVTVA PADTLTVRVI GDDAAAVPTD RANLVWRAAE MLAAEGGVAP NVEIVIEKGI 130 140 150 160 170 180 PVAGGMAGGS ADAAAALVAL NSLWKLDFSR PDLDAFAARL GSDVPFSLHG GTALGTGRGE 190 200 210 220 230 240 QLVPVLTRRT FHWVLALAKG GLSTPVVFRE LDKLRAEGTP NRLGTADELI HALTTGDPHV 250 260 270 280 290 300 LAPLLGNDLQ AAALSLNPDL RRTLRAGVEA GALAGIVSGS GPTCAFLCAD AQSAVEVSAE 310 320 330 LAGAGVCRTV RVASGPVPGA RILDNAAKGQ H

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH35060.1.
RefSeq	YP_002767799.1. NC_012490.1.
3D structure databases
ProteinModelPortal	C1A375.
ModBase	Search...
Protein-protein interaction databases
STRING	C1A375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7709818.
GenomeReviews	Gene locus RER_43520 in contig AP008957_GR.
KEGG	rer:RER_43520.
PATRIC	23195287. VBIRhoEry66701_4900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KINLHLG.
ProtClustDB	PRK03188.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C1A375_RHOE4

Accession

Primary (citable) accession number: C1A375

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)