C1A1W3 (C1A1W3_RHOE4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL BAH34598.1
Ordered Locus Names:	RER_38900

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 EMBL BAH34598.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	410	1	Nucleophile By similarity HAMAP MF_00685
	Active site	463	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	C1A1W3 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 9EFDE1BBB33210DA	FASTA	734	81,515
	10 20 30 40 50 60 MMPTEKSPSG IAPDNHDLEL LARGEHHDPH SVLGAHPHAD GTVLRALRPN AESVSAVIGG 70 80 90 100 110 120 KTFPLEHVSH GIFAALVPYP DLIDYRYSVT YPGGHTVLAA DPYRFLPTLG ELDLHLFGEG 130 140 150 160 170 180 RHERLWDILG AHPRSYETPD GPVDGTSFAV WAPAARGVTV VGDFDGWSGQ FAPMRVLGST 190 200 210 220 230 240 GVWELFLPDI AVGSLYKFRV HGHDGSVRDK ADPMAFGTEV PPATASRVTT SSHEWNDAAW 250 260 270 280 290 300 LETRAGVDPS QSPMSVYEVH LGSWRPGLDY RQMAEQLAVY VTETGFTHVE LLPVSEHPFG 310 320 330 340 350 360 GSWGYQVTSY YAPTSRFGTP DDFRWFVDHL HAAGIGVIVD WVPAHFPKDE WALARFDGTP 370 380 390 400 410 420 LYEHADPRRG EQLDWGTYVF DFGRREVRNF LVANALFWLE EFHIDGLRVD AVASMLYLDY 430 440 450 460 470 480 SRPEGGWTPN IHGGRENLEA VAFLQEMNAT VHKNHRGVVT VAEESTAWPG VTRATNVGGL 490 500 510 520 530 540 GFNMKWNMGW MHDTLGFLGH DPVHRTYHHH EITFSLMYAW SENYLLPISH DEVVHGKGTL 550 560 570 580 590 600 WTRMPGNDFA KASGLRSLLA YMWAHPGKQL LFMGQEFGQS AEWSEERGLD WWQLDDQYTG 610 620 630 640 650 660 TLHRGVQRLV GDLNSAYRRH PAMWTLDTSP GGYSWIDAND TENNVLSFLR YGTDGSIVAC 670 680 690 700 710 720 LFNFSGSEHS SYRVGLPEPG RWVEILNTDA EVYGGSGVGN LGAVTATSRS WHGRPASAQV 730 ALPAAGAIWI SLER

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH34598.1.
RefSeq	YP_002767337.1. NC_012490.1.
3D structure databases
ProteinModelPortal	C1A1W3.
ModBase	Search...
Protein-protein interaction databases
STRING	C1A1W3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7713172.
GenomeReviews	Gene locus RER_38900 in contig AP008957_GR.
KEGG	rer:RER_38900.
PATRIC	23194311. VBIRhoEry66701_4423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WEIFAPD.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C1A1W3_RHOE4

Accession

Primary (citable) accession number: C1A1W3

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)