C1A1H0 (CYSH_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	RER_37470

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite By similarity. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000202398

Sequences

	Sequence		Length	Mass (Da)
	C1A1H0 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: E4FFE03516C6A99D	FASTA	247	26,318
	10 20 30 40 50 60 MTVDISRATA DELRSIAERG AVELDGASAQ ELLQWTEDTF GDGASAATGD RNGYIVASNM 70 80 90 100 110 120 QDGVLVHLAA QVHPGVDVLF LDTGYHFPET IGTRDAVEQV YGVNVINARA LASVAEQDVA 130 140 150 160 170 180 EGKDLFARDP NRCCALRKVA PLKQTLSGYS AWVTGIRRVE APTRANAPLI SFDEAFGLVK 190 200 210 220 230 240 INPIAPWSDE EMQNYIDTNS ILVNPLVDEG YPSIGCAPCT SKPAPGSDPR SGRWAGASKT ECGLHAS

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH34455.1.
RefSeq	YP_002767194.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1A1H0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7713363.
GenomeReviews	Gene locus RER_37470 in contig AP008957_GR.
KEGG	rer:RER_37470.
PATRIC	23193989. VBIRhoEry66701_4269.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FEETLAY.
ProtClustDB	PRK02090.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. CysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_RHOE4

Accession

Primary (citable) accession number: C1A1H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program