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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Rhodococcus erythropolis (strain PR4 / NBRC 100887)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi269 – 2691Manganese 2UniRule annotation
Metal bindingi274 – 2741Manganese 1UniRule annotation
Metal bindingi274 – 2741Manganese 2UniRule annotation
Active sitei281 – 2811UniRule annotation
Metal bindingi292 – 2921Manganese 2UniRule annotation
Metal bindingi351 – 3511Manganese 1UniRule annotation
Metal bindingi353 – 3531Manganese 1UniRule annotation
Metal bindingi353 – 3531Manganese 2UniRule annotation
Active sitei355 – 3551UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciRERY234621:GHDE-3657-MONOMER.

Protein family/group databases

MEROPSiM17.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:RER_36150
OrganismiRhodococcus erythropolis (strain PR4 / NBRC 100887)
Taxonomic identifieri234621 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
ProteomesiUP000002204 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Probable cytosol aminopeptidasePRO_1000203838Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi234621.RER_36150.

Structurei

3D structure databases

ProteinModelPortaliC1A138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiRKGMESE.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1A138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRTARSLG PDLVLAGTVA KRAEILVVGL TSGPDGPEIA LSEGIVAEDV
60 70 80 90 100
LAEILDSLIA VGATGKPEQL TRVPAPSALS VTSVLAVGLG SADKLDSEQI
110 120 130 140 150
RKSAGAAARS LSGIDTVATT LSILDLGAAA EGFALGAYSF TEFKSSMTAP
160 170 180 190 200
GPDSQPLARV ELLVPSPRTK ETKATLARSA AIAEAVATAR EFVNTPPSHL
210 220 230 240 250
YPAEFAARAK ALGVEAGLTV QVLDEKALEK GGYGGIIGVG KGSSRQPRLV
260 270 280 290 300
RLEYASKKRG ARKVALVGKG ITFDTGGISI KPAAGMENMT SDMGGAAAVI
310 320 330 340 350
STVVLAAKLG LPVNVVAYVP MAENMPSATA QRPGDVLTQY GGITIEVVNT
360 370 380 390 400
DAEGRLILAD AMVRAGEDNP DYMIDTATLT GAQMVALGNR TPGVMGTDEF
410 420 430 440 450
RDRVASISQS VGENGWAMPL PEELRGDLDS KVADMANVTP HRWGGMLVAA
460 470 480 490 500
HYLKEFVPEG VQWAHIDVAG PAYNTSGPWG YTGKGGTGVP VRTMISVLED

IAVNG
Length:505
Mass (Da):52,012
Last modified:May 26, 2009 - v1
Checksum:i437D2EFC0C97E40A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008957 Genomic DNA. Translation: BAH34323.1.
RefSeqiWP_020908114.1. NC_012490.1.
YP_002767062.1. NC_012490.1.

Genome annotation databases

EnsemblBacteriaiBAH34323; BAH34323; RER_36150.
GeneIDi7715119.
KEGGirer:RER_36150.
PATRICi23193706. VBIRhoEry66701_4131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008957 Genomic DNA. Translation: BAH34323.1.
RefSeqiWP_020908114.1. NC_012490.1.
YP_002767062.1. NC_012490.1.

3D structure databases

ProteinModelPortaliC1A138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234621.RER_36150.

Protein family/group databases

MEROPSiM17.950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH34323; BAH34323; RER_36150.
GeneIDi7715119.
KEGGirer:RER_36150.
PATRICi23193706. VBIRhoEry66701_4131.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiRKGMESE.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciRERY234621:GHDE-3657-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PR4 / NBRC 100887.

Entry informationi

Entry nameiAMPA_RHOE4
AccessioniPrimary (citable) accession number: C1A138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.