C1A0Y3 (RSMH_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	RER_35600

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387076
Regions
	Region	48 – 50	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	118	1	S-adenosyl-L-methionine By similarity
	Binding site	125	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1A0Y3 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 5BD2830427BBE551	FASTA	332	36,023
	10 20 30 40 50 60 MNEPSDADGF AHVPVLLHRA DDLLGPAITA VGDGGEGAYM IDATLGLGGH SEFFLGKYPG 70 80 90 100 110 120 LHLIGLDRDT NALRIASERL APFADRTTFV HTTYDGIEDA LEQAGLPSTE SVHAILFDLG 130 140 150 160 170 180 VSSMQLDEAD RGFAYSIDAP LDMRMDQTVG LTAAEVLNTY SHGDLARILS TYGEERFAGK 190 200 210 220 230 240 IASEILRQRE REPFTTSAAL VELLYRTIPA ATRRTGGHPA KRTFQALRIE VNAELDSLRA 250 260 270 280 290 300 AVPAGLDALA VGGRVVFMSY QSLEDRVVKA ELVPRSKSTS PEGLPVELPG MGPEFRILTR 310 320 330 GAERASEQEI EENPRSAPVR LRAAERISRR RA

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH34268.1.
RefSeq	YP_002767007.1. NC_012490.1.
3D structure databases
ProteinModelPortal	C1A0Y3.
ModBase	Search...
Protein-protein interaction databases
STRING	C1A0Y3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7712611.
GenomeReviews	Gene locus RER_35600 in contig AP008957_GR.
KEGG	rer:RER_35600.
PATRIC	23193596. VBIRhoEry66701_4076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NPATRAF.
ProtClustDB	PRK00050.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_RHOE4

Accession

Primary (citable) accession number: C1A0Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program