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C1A0S6 (BIOD_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:RER_35030
OrganismRhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]
Taxonomic identifier234621 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000205213

Regions

Nucleotide binding12 – 176ATP By similarity
Nucleotide binding110 – 1134ATP By similarity
Nucleotide binding201 – 2033ATP By similarity

Sites

Metal binding121Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding501Magnesium 2 By similarity
Metal binding1101Magnesium 2 By similarity
Binding site411Substrate By similarity
Binding site501ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C1A0S6 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: FC1BA3EE550859A2

FASTA22722,840
        10         20         30         40         50         60 
MSIFLVTGTS TDVGKTVVTA ALASVALQSG KSVAVLKPAQ TGVDVDGAGD LAEIERLTGG 

        70         80         90        100        110        120 
GVTLVELARY PEPLAPDTAA RRSGLPLLAL DDVVRVARDL DTTHDLTLIE GAGGLLVRLG 

       130        140        150        160        170        180 
VDGFTARDLA AALAAPVVLV VASGLGTLNH TALTVEALGA SGVECAGLVI GSWPQEPDLA 

       190        200        210        220 
ERCNREDLVS VSGVPLLGLM PAGSGSESVQ EFAQTAWGAL GKSPLAI

« Hide

References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008957 Genomic DNA. Translation: BAH34211.1.
RefSeqYP_002766950.1. NC_012490.1.

3D structure databases

ProteinModelPortalC1A0S6.
ModBaseSearch...

Protein-protein interaction databases

STRINGC1A0S6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7712582.
GenomeReviewsGene locus RER_35030 in contig AP008957_GR.
KEGGrer:RER_35030.
PATRIC23193482. VBIRhoEry66701_4019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAAEAVGWC.
ProtClustDBPRK00090.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_RHOE4
AccessionPrimary (citable) accession number: C1A0S6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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