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C0ZZZ0

- MASZ_RHOE4

UniProt

C0ZZZ0 - MASZ_RHOE4

Protein

Malate synthase G

Gene

glcB

Organism
Rhodococcus erythropolis (strain PR4 / NBRC 100887)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi435 – 4351MagnesiumUniRule annotation
    Binding sitei435 – 4351GlyoxylateUniRule annotation
    Metal bindingi463 – 4631MagnesiumUniRule annotation
    Binding sitei544 – 5441Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei634 – 6341Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRERY234621:GHDE-3253-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:RER_32170
    OrganismiRhodococcus erythropolis (strain PR4 / NBRC 100887)
    Taxonomic identifieri234621 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000002204: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_1000212377Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei620 – 6201Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi234621.RER_32170.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni460 – 4634Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C0ZZZ0-1 [UniParc]FASTAAdd to Basket

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    MTERVQVGSL QVAKVLYDFV VEEALPGTGV DAETFWAGAD KVITELAPKN    50
    RDLLAKRDDL QAQIDQWHRD HAGAPIDAAA YKAFLQEIGY LLPTPAEFSV 100
    TTANVDTEIT STAGPQLVVP ILNARFALNA SNARWGSLYD ALYGTNAISE 150
    ENGAEKGSGY NKVRGDKVIA WAREFLDAAA PLASGSHADS TKYVIDGSEL 200
    KITLADGTVT TLAQPEKFVG YLGAKDAPTS ILLLNNGLHA EIQIDASSPI 250
    GSTDAAGVKD VVLESAVTTI MDFEDSVAAV DADDKVVGYR NWLGLNRGDL 300
    SEEIKKGAKS FTRTLNGDRK YTAVDGSELS LHGRSLLFVR NVGHLMTNPA 350
    ILDADGNEVP EGILDALITS TCAVHGLSIS DANGPLDNSR TGSIYIVKPK 400
    QHGPEEVAFT TELFGRVEQV LGLPENTLKV GIMDEERRTT VNLAACIKEA 450
    VDRVVFINTG FLDRTGDEIH TSMEAGAMVR KAEMKKQKWI GAYEDWNVDT 500
    GLACGLQGKA QIGKGMWAMT ELMADMLEQK IGHPKAGANT AWVPSPTGAT 550
    LHATHYHRVD VFAVQDSLKG KPRASVDDIL TIPLAPSTDW TDAEKKEELD 600
    NNCQSILGYV VRWIDAGVGC SKVPDIHDVA LMEDRATLRI SSQLLANWLR 650
    HGIVSESDVV SALERMAPVV DRQNEGDAEY RNMAPDFDSN IAFQAAKELI 700
    LEGAKQPSGY TEPILHRRRR EYKAANA 727
    Length:727
    Mass (Da):78,695
    Last modified:May 26, 2009 - v1
    Checksum:iBD7F5753677FE3C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008957 Genomic DNA. Translation: BAH33925.1.
    RefSeqiYP_002766664.1. NC_012490.1.

    Genome annotation databases

    EnsemblBacteriaiBAH33925; BAH33925; RER_32170.
    GeneIDi7709397.
    KEGGirer:RER_32170.
    PATRICi23192880. VBIRhoEry66701_3723.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008957 Genomic DNA. Translation: BAH33925.1 .
    RefSeqi YP_002766664.1. NC_012490.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 234621.RER_32170.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH33925 ; BAH33925 ; RER_32170 .
    GeneIDi 7709397.
    KEGGi rer:RER_32170.
    PATRICi 23192880. VBIRhoEry66701_3723.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci RERY234621:GHDE-3253-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
      Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PR4 / NBRC 100887.

    Entry informationi

    Entry nameiMASZ_RHOE4
    AccessioniPrimary (citable) accession number: C0ZZZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3