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Protein

Malate synthase G

Gene

glcB

Organism
Rhodococcus erythropolis (strain PR4 / NBRC 100887)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi435MagnesiumUniRule annotation1
Binding sitei435GlyoxylateUniRule annotation1
Metal bindingi463MagnesiumUniRule annotation1
Binding sitei544Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei634Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:RER_32170
OrganismiRhodococcus erythropolis (strain PR4 / NBRC 100887)
Taxonomic identifieri234621 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000002204 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002123771 – 727Malate synthase GAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei620Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi234621.RER_32170.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni460 – 463Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

C0ZZZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERVQVGSL QVAKVLYDFV VEEALPGTGV DAETFWAGAD KVITELAPKN
60 70 80 90 100
RDLLAKRDDL QAQIDQWHRD HAGAPIDAAA YKAFLQEIGY LLPTPAEFSV
110 120 130 140 150
TTANVDTEIT STAGPQLVVP ILNARFALNA SNARWGSLYD ALYGTNAISE
160 170 180 190 200
ENGAEKGSGY NKVRGDKVIA WAREFLDAAA PLASGSHADS TKYVIDGSEL
210 220 230 240 250
KITLADGTVT TLAQPEKFVG YLGAKDAPTS ILLLNNGLHA EIQIDASSPI
260 270 280 290 300
GSTDAAGVKD VVLESAVTTI MDFEDSVAAV DADDKVVGYR NWLGLNRGDL
310 320 330 340 350
SEEIKKGAKS FTRTLNGDRK YTAVDGSELS LHGRSLLFVR NVGHLMTNPA
360 370 380 390 400
ILDADGNEVP EGILDALITS TCAVHGLSIS DANGPLDNSR TGSIYIVKPK
410 420 430 440 450
QHGPEEVAFT TELFGRVEQV LGLPENTLKV GIMDEERRTT VNLAACIKEA
460 470 480 490 500
VDRVVFINTG FLDRTGDEIH TSMEAGAMVR KAEMKKQKWI GAYEDWNVDT
510 520 530 540 550
GLACGLQGKA QIGKGMWAMT ELMADMLEQK IGHPKAGANT AWVPSPTGAT
560 570 580 590 600
LHATHYHRVD VFAVQDSLKG KPRASVDDIL TIPLAPSTDW TDAEKKEELD
610 620 630 640 650
NNCQSILGYV VRWIDAGVGC SKVPDIHDVA LMEDRATLRI SSQLLANWLR
660 670 680 690 700
HGIVSESDVV SALERMAPVV DRQNEGDAEY RNMAPDFDSN IAFQAAKELI
710 720
LEGAKQPSGY TEPILHRRRR EYKAANA
Length:727
Mass (Da):78,695
Last modified:May 26, 2009 - v1
Checksum:iBD7F5753677FE3C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008957 Genomic DNA. Translation: BAH33925.1.
RefSeqiWP_020907832.1. NC_012490.1.

Genome annotation databases

EnsemblBacteriaiBAH33925; BAH33925; RER_32170.
GeneIDi7709397.
KEGGirer:RER_32170.
PATRICi23192880. VBIRhoEry66701_3723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008957 Genomic DNA. Translation: BAH33925.1.
RefSeqiWP_020907832.1. NC_012490.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234621.RER_32170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH33925; BAH33925; RER_32170.
GeneIDi7709397.
KEGGirer:RER_32170.
PATRICi23192880. VBIRhoEry66701_3723.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_RHOE4
AccessioniPrimary (citable) accession number: C0ZZZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.