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C0ZZA8 (C0ZZA8_RHOE4) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036 EMBL BAH33693.1
Ordered Locus Names:RER_29850
OrganismRhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]
Taxonomic identifier234621 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5711Zinc By similarity HAMAP MF_00036
Metal binding5751Zinc By similarity HAMAP MF_00036
Metal binding6741Zinc By similarity HAMAP MF_00036
Metal binding6781Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
C0ZZA8 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: FC02489C458F9DCA

FASTA89095,771
        10         20         30         40         50         60 
MQTHEIRRRF LDHFVKAGHT EVPSASLILD DPNLLFVNAG MVPFVPFFLG QQTPSYSRAT 

        70         80         90        100        110        120 
SVQKCVRTLD IEEVGITTRH NTFFQMAGNF SFGDYFKKEA ISHAWQLLTG SVEDGGYGID 

       130        140        150        160        170        180 
PTRLWATVYL DDDEAFSIWR DEVGVPEERI QRRGMKDNYW SMGIPGPCGP CSEIFYDRGA 

       190        200        210        220        230        240 
EYGVEGGPEA DEDRYIEIWN LVFMQNERGE GTNKGDYPIL GPLPKKNIDT GMGVERVAFL 

       250        260        270        280        290        300 
LQGVENVYET DLLRPVIDKA AELTGSVYGT DHTSDVRFRV IADHARTAAM LIADGVNPGN 

       310        320        330        340        350        360 
YGRGYVLRRL LRRIVRSAKL LGSEQPTMRE FMIVVRDTMA PSYPVLETDF TRIENVAVGE 

       370        380        390        400        410        420 
ETAFLKTLAS GSALFENAAE TVKSSGKSVI AGDQAFALHD TYGFPIDLTL EMASEAGLTV 

       430        440        450        460        470        480 
DEDGFRTLMA EQRKRAKDDA QARKHAHADL TVYKELLDRG ATEFTGFDEL VSEAHVLALI 

       490        500        510        520        530        540 
VDGVRVPVAK AGQDVEVILD RSPLYAESGG QIADIGSLTA PGLEAKVNDV QKIAKKVWTH 

       550        560        570        580        590        600 
KVTVQKGEIT EGDVVLAHVD AEWRKGATQG HSGTHMVHAA LRQVLGPNAV QAGSLNKPGY 

       610        620        630        640        650        660 
LRFDFSWQGA LSDSQKQDIE ALTNEAVASN YAVNTFVTDL DKAKAMGAMA LFGENYGDEV 

       670        680        690        700        710        720 
RVVEIGGPFS MELCGGTHVG SSAQIGQVTL LGEQSVGSGI RRVEAYVGLD SYRYLAKERA 

       730        740        750        760        770        780 
LLAGLSSSLK VPSEEVPARV EALVERLKVA EKELEQTKAK AVLAAAGEYV AKAKRIGSVL 

       790        800        810        820        830        840 
VIAEPAPAGV GGNDLRSLVT DIKGRLGSEP AVVALLGDVD GKVPFVVASS KAAQGLGVKA 

       850        860        870        880        890 
GELVASFGPK IGGRGGGKPD MAQGSGSDSA GIPAALDAIR DRVGELAGGS

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References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008957 Genomic DNA. Translation: BAH33693.1.
RefSeqYP_002766432.1. NC_012490.1.

3D structure databases

ProteinModelPortalC0ZZA8.
ModBaseSearch...

Protein-protein interaction databases

STRINGC0ZZA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7709262.
GenomeReviewsGene locus RER_29850 in contig AP008957_GR.
KEGGrer:RER_29850.
PATRIC23192402. VBIRhoEry66701_3486.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAGESKTDQ.
ProtClustDBPRK00252.

Family and domain databases

HAMAPMF_00036_B. Ala_tRNA_synth_B.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_synth_euk/bac.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC0ZZA8_RHOE4
AccessionPrimary (citable) accession number: C0ZZA8
Entry history
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info