C0ZYV9 (DXS_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	RER_28360

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000205074

Sequences

	Sequence		Length	Mass (Da)
	C0ZYV9 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: DAE8C4E2D09FD949	FASTA	648	68,293
	10 20 30 40 50 60 MGVLARIQGP DDLRQLSHAE MTELADEIRE FLVLKVAATG GHLGPNLGVV ELTLALHRIF 70 80 90 100 110 120 DSPQDAIIFD TGHQAYVHKI LTGRQDQFDT LRKQGGLSGY PCRAESEHDW VESSHASAAL 130 140 150 160 170 180 SYADGLAKAF ALTGQNRHVV AVVGDGALTG GMCWEALNNI AAGKDRSVVI VVNDNGRSYA 190 200 210 220 230 240 PTIGGLADHL SALRTAPSYE RALDSGRRMV KRLPWVGRTA YSVLHGMKAG LKDAVSPQVM 250 260 270 280 290 300 FTDLGIKYLG PVDGHDEAAM ESALRRAKAY GGPVIVHAVT RKGNGYAHAE NDVADQMHAT 310 320 330 340 350 360 GVIDPVTGRG TKSSAPDWTS VFSAALIEQA SRREDIVAIT AAMAGPTGLA AFGEKFPDRI 370 380 390 400 410 420 FDVGIAEQHA MTSAAGLALG GLHPVVAIYS TFLNRAFDQL LMDVALLKQP VTVVLDRAGV 430 440 450 460 470 480 TGVDGASHNG VWDLSLLGII PGIRVAAPRD ADTLREELDE ALLVDDGPTV VRFPKGAVPE 490 500 510 520 530 540 AIPAVKRLDG MVDVLKASEG ERGDVLLVAV GPFASLALEI AERLDKQGIS VAVVDPRWVL 550 560 570 580 590 600 PVADSLVKMA DKYALVVTIE DGGLHGGIGS TVSAAMRAAG VHTSCRDMGV PQQFLDHASR 610 620 630 640 EAIHKELGLT AQDLSRKITG WVAGMGSVGV HVQEDASSAS AQGEVAQG

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH33544.1.
RefSeq	YP_002766283.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZYV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7713193.
GenomeReviews	Gene locus RER_28360 in contig AP008957_GR.
KEGG	rer:RER_28360.
PATRIC	23192082. VBIRhoEry66701_3332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QYDISYM.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_RHOE4

Accession

Primary (citable) accession number: C0ZYV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program