C0ZXT2 (RNH2_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	RER_24590

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Ribonuclease HII HAMAP MF_00052_B		PRO_1000202290
Sites
	Metal binding	35	1	Divalent metal cation By similarity
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	129	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0ZXT2 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 5441D0E411AF88B5	FASTA	226	23,679
	10 20 30 40 50 60 MSSWPPRVVI RRSAGLRTME SALARTGLGP VAGVDEAGRG ACAGPLVIAA CVLAPKPQAA 70 80 90 100 110 120 LARLDDSKKL TERAREELFP AIKRLALAWS VVSVPAAEVD QIGIHVANIE GMRRAVAGLD 130 140 150 160 170 180 LEPGYVLTDG FRVPGLTAPS LPVIGGDGAA ACIAAASVLA KVTRDRVMTE MDETHPGYGF 190 200 210 220 AIHKGYSTPL HMDALDELGP CPEHRMTYAN VVAAGVRLEQ RAGRTG

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH33167.1.
RefSeq	YP_002765906.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZXT2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7712124.
GenomeReviews	Gene locus RER_24590 in contig AP008957_GR.
KEGG	rer:RER_24590.
PATRIC	23191344. VBIRhoEry66701_2964.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_RHOE4

Accession

Primary (citable) accession number: C0ZXT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program