C0ZXL8 (C0ZXL8_RHOE4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 1 HAMAP MF_00012
Short name=DAD 1 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD EMBL BAH33103.1
Synonyms:	ilvD1 HAMAP MF_00012
Ordered Locus Names:	RER_23950

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL BAH33103.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	197	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	C0ZXL8 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: D722041CB015D7A3	FASTA	613	64,265
	10 20 30 40 50 60 MPPLRSRTTT IGRNAAGARS LWRATGMTDS DFGKPIVAVA NSYTQFVPGH VHLKNVGEIV 70 80 90 100 110 120 AEAISAAGGV AREFHTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMANA HTADALVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNIPT VFVSGGPMEA GKAVVVGGVA QAPTDLITAI SASANDAVSD 190 200 210 220 230 240 EGLDEVERSA CPTCGSCSGM FTANSMNCLT EALGLALPGN GSTLATHKAR HALFSRAGTT 250 260 270 280 290 300 VVEAALKYYR DGDESVLPRN IATPAAFRNA MALDVAMGGS TNTVLHTLAA AQEGEVDFDL 310 320 330 340 350 360 DTIDAISRKV PCLSKVSPNS DYHMEDVHRA GGIPALLGEL RRGGLLETDV STVHTKSFDQ 370 380 390 400 410 420 WLDDWDIRSG KATDEAIELF HAAPGGVRTI EPFSTDNRWS ALDTDAAGGC IRDVENAYTV 430 440 450 460 470 480 EGGLVVLRGN IALDGAILKT AGIDEELFSF QGPALVVESQ EEAVSVILQK KIKAGDVLVV 490 500 510 520 530 540 RYEGPKGGPG MQEMLHPTAF LKGAGLGKEC ALITDGRFSG GTSGLSIGHI SPEAAAGGVI 550 560 570 580 590 600 GLVQNGDQVR IDVASRTLEI LVDDDVLAER RAKMEASERP WQPVDRQRTV TTALRAYAAL 610 ATSADKGAVR YVP

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH33103.1.
RefSeq	YP_002765842.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZXL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7709918.
GenomeReviews	Gene locus RER_23950 in contig AP008957_GR.
KEGG	rer:RER_23950.
PATRIC	23191214. VBIRhoEry66701_2901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C0ZXL8_RHOE4

Accession

Primary (citable) accession number: C0ZXL8

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)