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C0ZV17

- MEND_RHOE4

UniProt

C0ZV17 - MEND_RHOE4

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Rhodococcus erythropolis (strain PR4 / NBRC 100887)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciRERY234621:GHDE-1723-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:RER_17040
OrganismiRhodococcus erythropolis (strain PR4 / NBRC 100887)
Taxonomic identifieri234621 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000002204: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5375372-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_1000215861Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi234621.RER_17040.

Structurei

3D structure databases

ProteinModelPortaliC0ZV17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiWRSAVCR.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

C0ZV17-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPSTAQATA VVDELVRGGV REVVLCPGSR NAPLAFALQA ADLDGRLRLH
60 70 80 90 100
MRIDERTAGF LALGLAIAGK RPVPIVMTSG TAVANLGPAV LEANYARVPL
110 120 130 140 150
VVLSANRPYE MLGTGANQTV EQLGLFGSQV RATISLGLAE DDSTQNSQWR
160 170 180 190 200
SAVCRVLAAA RGTRSGNAGP VHFDIPLREP LVPDVHVHGP VPEGRPGGAA
210 220 230 240 250
WTTTQNATLD VPVDLDLTAD TVVISGHGSA LRPELAGLPT VAEPTAPMHG
260 270 280 290 300
IALHPLALSQ LKPKQAIITG RPTLHRQVSK VLADPSVDVY ALTTGPRWPD
310 320 330 340 350
VSGNVLATGT RAVVTGTPDP AWIARCAALT EHAETAVRKQ LDAHPKATGL
360 370 380 390 400
HVAAAVMDAL ADGDQLLLGA SNPVRDAALV SYPKPAVRVL SNRGVAGIDG
410 420 430 440 450
TVSAAVGAAL AYEGGRTVAL MGDLTFLHDA SGLLIGTGEP RPSDLTIVVA
460 470 480 490 500
NDDGGGIFEL LEQGDPQYAG VFERVFGTPH GMDLAALCAA YRVPHAAVTV
510 520 530
DALATTLAQP ANGIRVLEVA TDRSGLRELH ASVRAQL
Length:537
Mass (Da):55,603
Last modified:May 26, 2009 - v1
Checksum:i77FA0F6247E6CBF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008957 Genomic DNA. Translation: BAH32412.1.
RefSeqiYP_002765151.1. NC_012490.1.

Genome annotation databases

EnsemblBacteriaiBAH32412; BAH32412; RER_17040.
GeneIDi7715197.
KEGGirer:RER_17040.
PATRICi23189778. VBIRhoEry66701_2190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008957 Genomic DNA. Translation: BAH32412.1 .
RefSeqi YP_002765151.1. NC_012490.1.

3D structure databases

ProteinModelPortali C0ZV17.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 234621.RER_17040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH32412 ; BAH32412 ; RER_17040 .
GeneIDi 7715197.
KEGGi rer:RER_17040.
PATRICi 23189778. VBIRhoEry66701_2190.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OMAi WRSAVCR.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci RERY234621:GHDE-1723-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
    Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PR4 / NBRC 100887.

Entry informationi

Entry nameiMEND_RHOE4
AccessioniPrimary (citable) accession number: C0ZV17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3