C0ZSW0 (KATG_RHOE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	RER_12250

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Catalase-peroxidase HAMAP MF_01961		PRO_1000216226
Sites
	Active site	110	1	Proton acceptor By similarity
	Metal binding	272	1	Iron (heme axial ligand) By similarity
	Site	106	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	109 ↔ 231		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-257) By similarity
	Cross-link	231 ↔ 257		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-109) By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0ZSW0 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 89718B33D8DBE547	FASTA	740	80,838
	10 20 30 40 50 60 MSDSCPVAHD GNTASTSESE NPAIPSPTPT GNRPRTNRDW WPNQPDLSVL HAHSSKSNPM 70 80 90 100 110 120 GADFDYAQEF AKLDVEALKR DVIALMTASQ DWWPADYGHY GGLFVRMSWH AAGTYRIADG 130 140 150 160 170 180 RGGGGQGAQR FAPLNSWPDN ASLDKARRLL WPVKQKYGKQ VSWADLLVFA GNCALESMGF 190 200 210 220 230 240 TTFGFGFGRE DIWEPEEIYW GPEDTWLGDE RYSGDRELSG PLGAVQMGLI YVNPEGPNGQ 250 260 270 280 290 300 PDPVAAARDI RETFARMAMN DVETAALIAG GHTFGKTHGA GPADLVGPEP EGAPVEQQGL 310 320 330 340 350 360 GWKSAFGTGV GKDAITSGLE VVWTPTPTKW DNTFLEILYG YDWELTKSPA GAWQWIPKDG 370 380 390 400 410 420 AGAGTIPDPF DSSAGRTPTM LTTDLSLRLD PTYEKITRRW LDHPEEFAEE FAKAWYKLLH 430 440 450 460 470 480 RDMGPVTRYL GPWVPEPQLW QDPVPSADDQ LIGDADIAIL KSRLLDSGLS VSQLVSTAWA 490 500 510 520 530 540 SAASFRSTDM RGGANGARIR LEPQKNWEVN EPATLSAVLQ TLERVQQEFN QAGGAKVSLA 550 560 570 580 590 600 DLIVLGGTAA VEQAAKNAGQ DITVSFTPGR TDATQEQTDV DSFEVLEPRA DGFRNYLKGG 610 620 630 640 650 660 EKIPAEILLV DRAYMLSLTP PEVTVLVGGL RALNANFGKT GHGVFTDRPE TLTNDFFVNL 670 680 690 700 710 720 LDMGTEWKPS KTEENVYDGV DRATGDPKYT ATAVDLVFGS NSQLRALSEV YASEDAKQKF 730 740 AEDFAAAWTK VMDLDRFDVN

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH31933.1.
RefSeq	YP_002764672.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZSW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7713139.
GenomeReviews	Gene locus RER_12250 in contig AP008957_GR.
KEGG	rer:RER_12250.
PATRIC	23188774. VBIRhoEry66701_1689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_RHOE4

Accession

Primary (citable) accession number: C0ZSW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program