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C0ZRP8

- PCKG_RHOE4

UniProt

C0ZRP8 - PCKG_RHOE4

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Rhodococcus erythropolis (strain PR4 / NBRC 100887)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851SubstrateUniRule annotation
    Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
    Metal bindingi233 – 2331ManganeseUniRule annotation
    Binding sitei233 – 2331SubstrateUniRule annotation
    Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Active sitei277 – 2771UniRule annotation
    Metal bindingi300 – 3001ManganeseUniRule annotation
    Binding sitei392 – 3921GTPUniRule annotation
    Binding sitei423 – 4231GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2816GTPUniRule annotation
    Nucleotide bindingi518 – 5214GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRERY234621:GHDE-1108-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:RER_10900
    OrganismiRhodococcus erythropolis (strain PR4 / NBRC 100887)
    Taxonomic identifieri234621 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000002204: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_1000206233Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi234621.RER_10900.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni390 – 3923Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C0ZRP8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSATIPGLN GTDGTPPTQH KELLAWVQEV AELTQPDRVV FADGSDEEWD    50
    RLTTKLVEAG TFTRLNDEKK PNSFLGNSDP SDVARVESRT YICSKEEIDA 100
    GPTNNWMDPA EMRTLMGDLY RGCMRGRTMY VVPFCMGPLD AEDPKLGVEL 150
    TDSEYVVVSM RVMTRMGSKV LDKLGTDGFF VKALHSLGAP LADGQEDVAW 200
    PCNDTKYITH FPEDREIWSF GSGYGGNALL GKKCYSLRIA SAMAHDEGWL 250
    AEHMLILKLI SPEDKAYYIA AAFPSACGKT NLAMIQPTIP GWRAETLGDD 300
    IAWMRFGEDG QLYAVNPEFG FFGVAPGTNW SSNPNAMRTI DQGNTVFTNV 350
    ALTDDGDVWW EGLEGDPQHL IDWKGNEWTP ESGTHAAHPN SRYCTPMSQC 400
    PIMAPEWDDP KGVPISAILF GGRRKTTVPL VTEARDWQHG VFMGATVGSE 450
    QTAAAEGQVG TVRRDPMAML PFLGYNVGDY FQHWIDLGKS ADASKLPKVF 500
    YVNWFRRGDD GRFLWPGFGE NSRVLKWIVE RIEHKAAGID TPIGVVPTGS 550
    ALDIDGLDVS DADITEALAV NIDEWKAEIP LIEEWFDFVG EKLPTGIRDE 600
    FEALKQRLA 609
    Length:609
    Mass (Da):67,398
    Last modified:May 26, 2009 - v1
    Checksum:i9B8F734434C0EB4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008957 Genomic DNA. Translation: BAH31798.1.
    RefSeqiYP_002764537.1. NC_012490.1.

    Genome annotation databases

    EnsemblBacteriaiBAH31798; BAH31798; RER_10900.
    GeneIDi7710911.
    KEGGirer:RER_10900.
    PATRICi23188500. VBIRhoEry66701_1552.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008957 Genomic DNA. Translation: BAH31798.1 .
    RefSeqi YP_002764537.1. NC_012490.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 234621.RER_10900.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH31798 ; BAH31798 ; RER_10900 .
    GeneIDi 7710911.
    KEGGi rer:RER_10900.
    PATRICi 23188500. VBIRhoEry66701_1552.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci RERY234621:GHDE-1108-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
      Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PR4 / NBRC 100887.

    Entry informationi

    Entry nameiPCKG_RHOE4
    AccessioniPrimary (citable) accession number: C0ZRP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3