C0ZNG9 (C0ZNG9_RHOE4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAH31221.1
Ordered Locus Names:	RER_05130

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	978 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C0ZNG9 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 6AA135117D43C97B	FASTA	978	106,618
	10 20 30 40 50 60 MAKADNGSGQ GASGNPRRLV IVESPTKARK IAPYLGKDYV VEASVGHIRD LPRGAADVPA 70 80 90 100 110 120 KYKGESWARL GVNVDHDFEP LYVVSPEKKG KVAELKSLLK DADELFLATD PDREGEAIAW 130 140 150 160 170 180 HLLETLKPKI PVRRMVFHEI TEPAIRAAAE DTRELDTDLV DAQETRRILD RLYGYEVSPV 190 200 210 220 230 240 LWKKVMPKLS AGRVQSVATR VIVQRERERM AFKSASYWDI SATLDAGAEA SPRSFGARLV 250 260 270 280 290 300 SVDGSRVASG RDFGADGKLK GSAVTVLDEA YARRLAEALQ GVDLTVSSAE DKPYTRKPYP 310 320 330 340 350 360 PFMTSTLQQE AGRKLRFTSE RAMRIAQRLY ENGYITYMRT DSTTLSESAI AAARAQATEL 370 380 390 400 410 420 YGPEYVHPTP RQYTRKVKNA QEAHEAIRPA GDVFQTPGQL HSKLDSDEFK LYELIWQRTV 430 440 450 460 470 480 ASQMADARGT TLTLRISGTA GTGEDCTFSA SGRTIVFAGF LKAYVESVDE EAGGQSDDAE 490 500 510 520 530 540 SRLPVLVKGQ EVTATKLDPD GHSTNPPARY TEASLIKTLE ELGIGRPSTY SSIIKTILDR 550 560 570 580 590 600 GYVYKRGSAL VPSWVAFAVI GLLEVHFGRL VDFDFTAGME DDLDAIAGGR EQRGNWLSSF 610 620 630 640 650 660 YFGGETGAEG SVAREGGLKK MVGVNLEEID AREVNSIRLF DDAEGREVHV RVGRFGPYLE 670 680 690 700 710 720 RMVQNPDDPE GDLISQRANL PDDLPPDELT PEYAEKLFST PQEGRKLGVD PLTGHEIVAK 730 740 750 760 770 780 EGRFGPYVTE ILPEPPAEPE PDIVPVSAPE PEEISSGSTK TAVKKAAAKK APAKKAAKKV 790 800 810 820 830 840 AGPKPRTGSL LKSMDLATIT LEDALRLLSL PRVVGVDPES KEEITAQNGR YGPYLKKGTD 850 860 870 880 890 900 SRSLANEEQM FTVTLDEALK IYAEPKRRGR QASATPPLRE LGVDPVSEKP MVIKDGRFGP 910 920 930 940 950 960 YVTDGETNAS LRKDDDVESI SDARASELLA DRRARGPVKK KAAAKKAPAK KAAAKKTAAK 970 KAPAKKAAAK KAPAKKDS

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH31221.1.
RefSeq	YP_002763960.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZNG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7711764.
GenomeReviews	Gene locus RER_05130 in contig AP008957_GR.
KEGG	rer:RER_05130.
PATRIC	23187294. VBIRhoEry66701_0957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C0ZNG9_RHOE4

Accession

Primary (citable) accession number: C0ZNG9

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)