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C0ZN14 (C0ZN14_RHOE4) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172 EMBL BAH35422.1
Ordered Locus Names:RER_47140
OrganismRhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]
Taxonomic identifier234621 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6411Zinc By similarity HAMAP MF_00172
Metal binding6431Zinc By similarity HAMAP MF_00172
Metal binding7261Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
C0ZN14 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 15C034C8AF041898

FASTA75481,415
        10         20         30         40         50         60 
MTTTFTSTVL GSPRIGPHRE LKRATESYWA GRIDADALQA VARDLRRQGL ADLRDLGLDS 

        70         80         90        100        110        120 
IPVNTFSFYD QMLDTAVLLG ALPDRVASVA DPLDRYFAAA RGTDTIAPLE MTKWFDTNYH 

       130        140        150        160        170        180 
YLVPEISPST TFALDPSKVL SELAEALSDG IAARPVVIGP VTFLLLSKAV GTDEPLLSRL 

       190        200        210        220        230        240 
DELLPLYADL LGRLAKAGAS WVQFDEPALV GDRTDEEIEA ARAAYEQLSA LSKRPSILLA 

       250        260        270        280        290        300 
SYFGSLRDAL PALASTGVEG FAVDLVAGQD SIGSVPELAR KLIVAGVVDG RNIWRSDLDS 

       310        320        330        340        350        360 
ALSTLGALLG SVDTLAVSTS CSLLHVPYTL DAESDVDKAL RSWLAFGTEK VKEVVTLATA 

       370        380        390        400        410        420 
LTQGRESVED EFALARAAAS TRKDDRRLND ASVRGRLDAV LNSATGRAPA AERRAAQDEL 

       430        440        450        460        470        480 
LSLPPLPTTT IGSYPQTSKI RIARAALRKG EIDEAEYVNR MRAEIAEVVA LQEKLDLDVL 

       490        500        510        520        530        540 
VHGEPERNDM VQYFAEQLDG FFATANGWVQ SYGTRCVRPP ILYGDVSRPK QMTVDWITYA 

       550        560        570        580        590        600 
QSLTDKPVKG MLTGPVTILA WSFVRDDQPL ADSANQVALA IRDETVDLQA AGIGIIQVDE 

       610        620        630        640        650        660 
PALRELLPLR AEDQRAYLDW SVGAFRLATS GVADSTAIHT HLCYSEFGAV IGAIAGLDAD 

       670        680        690        700        710        720 
VTSIEAARSH MEVLDDLSSV GFDLGVGPGV YDIHSPRVPS VDEIAESLRE ALKAVPVERL 

       730        740        750 
WVNPDCGLKT RGPIEVEASL RNLVEAAKVV RAEL

« Hide

References

[1]"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4."
Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008957 Genomic DNA. Translation: BAH35422.1.
RefSeqYP_002768161.1. NC_012490.1.

3D structure databases

ProteinModelPortalC0ZN14.
ModBaseSearch...

Protein-protein interaction databases

STRINGC0ZN14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7710427.
GenomeReviewsGene locus RER_47140 in contig AP008957_GR.
KEGGrer:RER_47140.
PATRIC23196028. VBIRhoEry66701_5268.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMARNIWRAN.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC0ZN14_RHOE4
AccessionPrimary (citable) accession number: C0ZN14
Entry history
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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