C0ZMB1 (C0ZMB1_RHOE4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008

Gene names

Name:	thyA HAMAP MF_00008 EMBL BAH35169.1
Ordered Locus Names:	RER_44610

Organism

Rhodococcus erythropolis (strain PR4 / NBRC 100887) [Complete proteome] [HAMAP]

Taxonomic identifier

234621 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Subunit structure	Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. RuleBase RU004150 Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
Biological process	Nucleotide biosynthesis SAAS SAAS023451 HAMAP MF_00008 RuleBase RU000500
Cellular component	Cytoplasm HAMAP MF_00008
Molecular function	Methyltransferase HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451 EMBL BAH35169.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	149	1	By similarity HAMAP MF_00008

Sequences

	Sequence		Length	Mass (Da)
	C0ZMB1 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 272BE272712DE5B3	FASTA	266	30,037
	10 20 30 40 50 60 MTVSTAYEDL LRLVMETGTA KSDRTGTGTR SIFGHQMRFD LADGFPLVTT KKVHLKSIVN 70 80 90 100 110 120 ELLWFLRGDS NVAWLHEHGV SIWDEWADEN GELGPVYGVQ WRSWPTPSGE HIDQITQTVE 130 140 150 160 170 180 TLRSNPDSRR IIVSAWNVAD IPQMALAPCH AFFQFYVADG KLSCQLYQRS ADMFLGVPFN 190 200 210 220 230 240 IASYALLTHM MAQQAGLDVG EFVWTGGDCH IYDNHVDQVT EQLGREPLPL PTLKLNKRDS 250 260 IFDYTFEDVE IVDYQHHPAI KAPVAV

References

[1]

"Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4." Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PR4 / NBRC 100887.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008957 Genomic DNA. Translation: BAH35169.1.
RefSeq	YP_002767908.1. NC_012490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C0ZMB1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7713600.
GenomeReviews	Gene locus RER_44610 in contig AP008957_GR.
KEGG	rer:RER_44610.
PATRIC	23195513. VBIRhoEry66701_5012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMALAPC.
ProtClustDB	PRK01827.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C0ZMB1_RHOE4

Accession

Primary (citable) accession number: C0ZMB1

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)