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C0ZIF7 (SYC_BREBN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Ordered Locus Names:BBR47_01980
OrganismBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) [Complete proteome] [HAMAP]
Taxonomic identifier358681 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_1000199045

Regions

Motif31 – 4111"HIGH" region HAMAP-Rule MF_00041
Motif266 – 2705"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding291Zinc By similarity
Metal binding2091Zinc By similarity
Metal binding2341Zinc By similarity
Metal binding2381Zinc By similarity
Binding site2691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C0ZIF7 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 813F1022FA502311

FASTA46853,604
        10         20         30         40         50         60 
MGIQLTNTMT RRKEPFHTLE PGKVKMYVCG PTVYNYIHIG NARPAIVFDT LRRYLKYRGY 

        70         80         90        100        110        120 
EVTFVQNITD VDDKLIRVAN QEGVTVKEVA DRYTDAYNAD LKSLNVSPPD IQPRVMQTIP 

       130        140        150        160        170        180 
EIIEFVQGLI EKGFAYESEG DVYFRTGRFQ EYGKLSHQPL DDLQAGARVE INEKKEHPLD 

       190        200        210        220        230        240 
FVLWKAAKTG EVTWDSPWGE GRPGWHIECS AMALKFLGEE IDIHAGGTDL VFPHHENEIA 

       250        260        270        280        290        300 
QSECFTGKVF ARYWLHNGML NIDNEKMSKS LGNFLLARDL SEKFGGQLIR FFMLQGHYRN 

       310        320        330        340        350        360 
PINFSEELIE QAANGLDRIT TAYTNLSHRL DTARAEEPND QAQEQARIIG ELRERFIAEM 

       370        380        390        400        410        420 
DDDINTANAI TVIFDVVKEA NLYLRHQNVG ETEVRAYMDL LVELTEVLGL DIAEEQELLD 

       430        440        450        460 
SEIDALIEER TEARKARNFA RSDEIRDLLA AKGIVLEDTP QGVRWRRK 

« Hide

References

[1]"Brevibacillus brevis strain 47, complete genome."
Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H. expand/collapse author list , Udaka S., Tanikawa S., Fujita N.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 47 / JCM 6285 / NBRC 100599.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008955 Genomic DNA. Translation: BAH41175.1.
RefSeqYP_002769679.1. NC_012491.1.

3D structure databases

ProteinModelPortalC0ZIF7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING358681.BBR47_01980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH41175; BAH41175; BBR47_01980.
GeneID7720579.
KEGGbbe:BBR47_01980.
PATRIC21230608. VBIBreBre96763_0201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0215.
HOGENOMHOG000245250.
KOK01883.
OMAEPDEIAW.
OrthoDBEOG6RVFXC.

Enzyme and pathway databases

BioCycBBRE358681:GHYS-248-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_BREBN
AccessionPrimary (citable) accession number: C0ZIF7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries