C0ZIF7 (SYC_BREBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	BBR47_01980

Organism

Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) [Complete proteome] [HAMAP]

Taxonomic identifier

358681 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Brevibacillus › Brevibacillus brevis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_1000199045
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0ZIF7 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 813F1022FA502311	FASTA	468	53,604
	10 20 30 40 50 60 MGIQLTNTMT RRKEPFHTLE PGKVKMYVCG PTVYNYIHIG NARPAIVFDT LRRYLKYRGY 70 80 90 100 110 120 EVTFVQNITD VDDKLIRVAN QEGVTVKEVA DRYTDAYNAD LKSLNVSPPD IQPRVMQTIP 130 140 150 160 170 180 EIIEFVQGLI EKGFAYESEG DVYFRTGRFQ EYGKLSHQPL DDLQAGARVE INEKKEHPLD 190 200 210 220 230 240 FVLWKAAKTG EVTWDSPWGE GRPGWHIECS AMALKFLGEE IDIHAGGTDL VFPHHENEIA 250 260 270 280 290 300 QSECFTGKVF ARYWLHNGML NIDNEKMSKS LGNFLLARDL SEKFGGQLIR FFMLQGHYRN 310 320 330 340 350 360 PINFSEELIE QAANGLDRIT TAYTNLSHRL DTARAEEPND QAQEQARIIG ELRERFIAEM 370 380 390 400 410 420 DDDINTANAI TVIFDVVKEA NLYLRHQNVG ETEVRAYMDL LVELTEVLGL DIAEEQELLD 430 440 450 460 SEIDALIEER TEARKARNFA RSDEIRDLLA AKGIVLEDTP QGVRWRRK

References

[1]

"Brevibacillus brevis strain 47, complete genome." Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H. , Udaka S., Tanikawa S., Fujita N. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 47 / JCM 6285 / NBRC 100599.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008955 Genomic DNA. Translation: BAH41175.1.
RefSeq	YP_002769679.1. NC_012491.1.
3D structure databases
ProteinModelPortal	C0ZIF7.
ModBase	Search...
Protein-protein interaction databases
STRING	358681.BBR47_01980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAH41175; BAH41175; BBR47_01980.
GeneID	7720579.
KEGG	bbe:BBR47_01980.
PATRIC	21230608. VBIBreBre96763_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
Enzyme and pathway databases
BioCyc	BBRE358681:GHYS-4771-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BREBN

Accession

Primary (citable) accession number: C0ZIF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program