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C0ZG86

- SYI_BREBN

UniProt

C0ZG86 - SYI_BREBN

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Protein

Isoleucine--tRNA ligase

Gene
ileS, BBR47_38180
Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi892 – 8921Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi912 – 9121Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBRE358681:GHYS-3949-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BBR47_38180
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
ProteomesiUP000001877: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 925925Isoleucine--tRNA ligaseUniRule annotationPRO_1000189132Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi358681.BBR47_38180.

Structurei

3D structure databases

ProteinModelPortaliC0ZG86.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0ZG86-1 [UniParc]FASTAAdd to Basket

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MDYSKTLALP KTEFPMRGNL PSREPQMQAV WEEQNIYQQV LDRTKDRPSF    50
VLHDGPPYAN GDIHIGHALN KILKDFIVRY KSMAGFYAPY IPGWDTHGLP 100
IEQAIINAQG LDRRSIEVND FRQRCEEYAW SYIDKQRDQF KRLGVRGDWE 150
NPYVTLLPEY EANQIRVFGE MAKKGYIYKG LRCVYWSPSS ETALADAEIE 200
YKDKRSPSIY VSFQVADGKG KLDIETGVVI WTTTPWTLPA NLAISLHPEL 250
EYNVVKVDGR KFLVANGLIE AASKEIGWEG VEILATFKGQ ELEGVETQHP 300
FYDRKSPLIL GEHVTLDAGT GCVHTAPGHG EDDFNVGQKY NLGVLCPVDH 350
EGKMTNEAPG FEGLFYEDAN KVITEKLKEN GALLKLSFFT HSYPHDWRTK 400
KPVIYRATEQ WFASIDGFRT QMLEAIKNVK WIPHWGETRL ANMIADRGDW 450
CISRQRVWGV PIPIFYCKAC NEPIINDTTI NHVADLFRKE GSKVWFSREA 500
NELVPEGLSC TKCDCNDFRK ETDIMDVWFD SGSSHQAVLR ERGIAWPADM 550
YLEGSDQYRG WFNSSLSTGV AVYGTAPYKS VLSHGFALDG EGRKMSKSLG 600
NVIVPQQVID KMGADILRLW VASVDYQADV RISDAILNQI AEVYRKIRNT 650
FRFLLGNLDG FNPATDRVAY EELGELDRYV LAKAAKVAKR TRKAYDEYQF 700
HTVFHAVHNF CVIDLSAFYL DICKDRLYVE APDSLKRRAA QTVMYDCLLS 750
LVKLVAPLLP HTADEVWAFI PGVEEKSVQL TDMPEGDEQH LSFAAEAESK 800
WDAFLAIRDE VLKAMEEARR NKVFGNSVDA KLALYPQTEE VAKTLAAMDD 850
LADLFIVAHV DVHSGSAPAE AVQLEGIAAV VSAADGGKCE RCRVVKPDVG 900
TRESHASLCV RCADVVEQHY AHVTE 925
Length:925
Mass (Da):104,410
Last modified:May 26, 2009 - v1
Checksum:i4E716B1C7ED6E053
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008955 Genomic DNA. Translation: BAH44795.1.
RefSeqiWP_015892079.1. NC_012491.1.
YP_002773299.1. NC_012491.1.

Genome annotation databases

EnsemblBacteriaiBAH44795; BAH44795; BBR47_38180.
GeneIDi7719311.
KEGGibbe:BBR47_38180.
PATRICi21237936. VBIBreBre96763_3785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008955 Genomic DNA. Translation: BAH44795.1 .
RefSeqi WP_015892079.1. NC_012491.1.
YP_002773299.1. NC_012491.1.

3D structure databases

ProteinModelPortali C0ZG86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 358681.BBR47_38180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH44795 ; BAH44795 ; BBR47_38180 .
GeneIDi 7719311.
KEGGi bbe:BBR47_38180.
PATRICi 21237936. VBIBreBre96763_3785.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BBRE358681:GHYS-3949-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Brevibacillus brevis strain 47, complete genome."
    Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.
    , Udaka S., Tanikawa S., Fujita N.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 47 / JCM 6285 / NBRC 100599.

Entry informationi

Entry nameiSYI_BREBN
AccessioniPrimary (citable) accession number: C0ZG86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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