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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi889ZincUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBRE358681:G1G2J-3898-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BBR47_38180
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
Proteomesi
  • UP000001877 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891321 – 925Isoleucine--tRNA ligaseAdd BLAST925

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi358681.BBR47_38180

Structurei

3D structure databases

ProteinModelPortaliC0ZG86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C0ZG86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSKTLALP KTEFPMRGNL PSREPQMQAV WEEQNIYQQV LDRTKDRPSF
60 70 80 90 100
VLHDGPPYAN GDIHIGHALN KILKDFIVRY KSMAGFYAPY IPGWDTHGLP
110 120 130 140 150
IEQAIINAQG LDRRSIEVND FRQRCEEYAW SYIDKQRDQF KRLGVRGDWE
160 170 180 190 200
NPYVTLLPEY EANQIRVFGE MAKKGYIYKG LRCVYWSPSS ETALADAEIE
210 220 230 240 250
YKDKRSPSIY VSFQVADGKG KLDIETGVVI WTTTPWTLPA NLAISLHPEL
260 270 280 290 300
EYNVVKVDGR KFLVANGLIE AASKEIGWEG VEILATFKGQ ELEGVETQHP
310 320 330 340 350
FYDRKSPLIL GEHVTLDAGT GCVHTAPGHG EDDFNVGQKY NLGVLCPVDH
360 370 380 390 400
EGKMTNEAPG FEGLFYEDAN KVITEKLKEN GALLKLSFFT HSYPHDWRTK
410 420 430 440 450
KPVIYRATEQ WFASIDGFRT QMLEAIKNVK WIPHWGETRL ANMIADRGDW
460 470 480 490 500
CISRQRVWGV PIPIFYCKAC NEPIINDTTI NHVADLFRKE GSKVWFSREA
510 520 530 540 550
NELVPEGLSC TKCDCNDFRK ETDIMDVWFD SGSSHQAVLR ERGIAWPADM
560 570 580 590 600
YLEGSDQYRG WFNSSLSTGV AVYGTAPYKS VLSHGFALDG EGRKMSKSLG
610 620 630 640 650
NVIVPQQVID KMGADILRLW VASVDYQADV RISDAILNQI AEVYRKIRNT
660 670 680 690 700
FRFLLGNLDG FNPATDRVAY EELGELDRYV LAKAAKVAKR TRKAYDEYQF
710 720 730 740 750
HTVFHAVHNF CVIDLSAFYL DICKDRLYVE APDSLKRRAA QTVMYDCLLS
760 770 780 790 800
LVKLVAPLLP HTADEVWAFI PGVEEKSVQL TDMPEGDEQH LSFAAEAESK
810 820 830 840 850
WDAFLAIRDE VLKAMEEARR NKVFGNSVDA KLALYPQTEE VAKTLAAMDD
860 870 880 890 900
LADLFIVAHV DVHSGSAPAE AVQLEGIAAV VSAADGGKCE RCRVVKPDVG
910 920
TRESHASLCV RCADVVEQHY AHVTE
Length:925
Mass (Da):104,410
Last modified:May 26, 2009 - v1
Checksum:i4E716B1C7ED6E053
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA Translation: BAH44795.1
RefSeqiWP_015892079.1, NC_012491.1

Genome annotation databases

EnsemblBacteriaiBAH44795; BAH44795; BBR47_38180
GeneIDi29261062
KEGGibbe:BBR47_38180

Similar proteinsi

Entry informationi

Entry nameiSYI_BREBN
AccessioniPrimary (citable) accession number: C0ZG86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: March 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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