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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBRE358681:GHYS-3949-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BBR47_38180
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
ProteomesiUP000001877 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 925925Isoleucine--tRNA ligasePRO_1000189132Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi358681.BBR47_38180.

Structurei

3D structure databases

ProteinModelPortaliC0ZG86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiSYLGEHV.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0ZG86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSKTLALP KTEFPMRGNL PSREPQMQAV WEEQNIYQQV LDRTKDRPSF
60 70 80 90 100
VLHDGPPYAN GDIHIGHALN KILKDFIVRY KSMAGFYAPY IPGWDTHGLP
110 120 130 140 150
IEQAIINAQG LDRRSIEVND FRQRCEEYAW SYIDKQRDQF KRLGVRGDWE
160 170 180 190 200
NPYVTLLPEY EANQIRVFGE MAKKGYIYKG LRCVYWSPSS ETALADAEIE
210 220 230 240 250
YKDKRSPSIY VSFQVADGKG KLDIETGVVI WTTTPWTLPA NLAISLHPEL
260 270 280 290 300
EYNVVKVDGR KFLVANGLIE AASKEIGWEG VEILATFKGQ ELEGVETQHP
310 320 330 340 350
FYDRKSPLIL GEHVTLDAGT GCVHTAPGHG EDDFNVGQKY NLGVLCPVDH
360 370 380 390 400
EGKMTNEAPG FEGLFYEDAN KVITEKLKEN GALLKLSFFT HSYPHDWRTK
410 420 430 440 450
KPVIYRATEQ WFASIDGFRT QMLEAIKNVK WIPHWGETRL ANMIADRGDW
460 470 480 490 500
CISRQRVWGV PIPIFYCKAC NEPIINDTTI NHVADLFRKE GSKVWFSREA
510 520 530 540 550
NELVPEGLSC TKCDCNDFRK ETDIMDVWFD SGSSHQAVLR ERGIAWPADM
560 570 580 590 600
YLEGSDQYRG WFNSSLSTGV AVYGTAPYKS VLSHGFALDG EGRKMSKSLG
610 620 630 640 650
NVIVPQQVID KMGADILRLW VASVDYQADV RISDAILNQI AEVYRKIRNT
660 670 680 690 700
FRFLLGNLDG FNPATDRVAY EELGELDRYV LAKAAKVAKR TRKAYDEYQF
710 720 730 740 750
HTVFHAVHNF CVIDLSAFYL DICKDRLYVE APDSLKRRAA QTVMYDCLLS
760 770 780 790 800
LVKLVAPLLP HTADEVWAFI PGVEEKSVQL TDMPEGDEQH LSFAAEAESK
810 820 830 840 850
WDAFLAIRDE VLKAMEEARR NKVFGNSVDA KLALYPQTEE VAKTLAAMDD
860 870 880 890 900
LADLFIVAHV DVHSGSAPAE AVQLEGIAAV VSAADGGKCE RCRVVKPDVG
910 920
TRESHASLCV RCADVVEQHY AHVTE
Length:925
Mass (Da):104,410
Last modified:May 25, 2009 - v1
Checksum:i4E716B1C7ED6E053
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH44795.1.
RefSeqiYP_002773299.1. NC_012491.1.

Genome annotation databases

EnsemblBacteriaiBAH44795; BAH44795; BBR47_38180.
KEGGibbe:BBR47_38180.
PATRICi21237936. VBIBreBre96763_3785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH44795.1.
RefSeqiYP_002773299.1. NC_012491.1.

3D structure databases

ProteinModelPortaliC0ZG86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi358681.BBR47_38180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH44795; BAH44795; BBR47_38180.
KEGGibbe:BBR47_38180.
PATRICi21237936. VBIBreBre96763_3785.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiSYLGEHV.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBBRE358681:GHYS-3949-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Brevibacillus brevis strain 47, complete genome."
    Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.
    , Udaka S., Tanikawa S., Fujita N.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 47 / JCM 6285 / NBRC 100599.

Entry informationi

Entry nameiSYI_BREBN
AccessioniPrimary (citable) accession number: C0ZG86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: May 25, 2009
Last modified: March 31, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.