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C0ZG86

- SYI_BREBN

UniProt

C0ZG86 - SYI_BREBN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBBRE358681:GHYS-3949-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BBR47_38180
    OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
    Taxonomic identifieri358681 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
    ProteomesiUP000001877: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 925925Isoleucine--tRNA ligasePRO_1000189132Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi358681.BBR47_38180.

    Structurei

    3D structure databases

    ProteinModelPortaliC0ZG86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C0ZG86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSKTLALP KTEFPMRGNL PSREPQMQAV WEEQNIYQQV LDRTKDRPSF    50
    VLHDGPPYAN GDIHIGHALN KILKDFIVRY KSMAGFYAPY IPGWDTHGLP 100
    IEQAIINAQG LDRRSIEVND FRQRCEEYAW SYIDKQRDQF KRLGVRGDWE 150
    NPYVTLLPEY EANQIRVFGE MAKKGYIYKG LRCVYWSPSS ETALADAEIE 200
    YKDKRSPSIY VSFQVADGKG KLDIETGVVI WTTTPWTLPA NLAISLHPEL 250
    EYNVVKVDGR KFLVANGLIE AASKEIGWEG VEILATFKGQ ELEGVETQHP 300
    FYDRKSPLIL GEHVTLDAGT GCVHTAPGHG EDDFNVGQKY NLGVLCPVDH 350
    EGKMTNEAPG FEGLFYEDAN KVITEKLKEN GALLKLSFFT HSYPHDWRTK 400
    KPVIYRATEQ WFASIDGFRT QMLEAIKNVK WIPHWGETRL ANMIADRGDW 450
    CISRQRVWGV PIPIFYCKAC NEPIINDTTI NHVADLFRKE GSKVWFSREA 500
    NELVPEGLSC TKCDCNDFRK ETDIMDVWFD SGSSHQAVLR ERGIAWPADM 550
    YLEGSDQYRG WFNSSLSTGV AVYGTAPYKS VLSHGFALDG EGRKMSKSLG 600
    NVIVPQQVID KMGADILRLW VASVDYQADV RISDAILNQI AEVYRKIRNT 650
    FRFLLGNLDG FNPATDRVAY EELGELDRYV LAKAAKVAKR TRKAYDEYQF 700
    HTVFHAVHNF CVIDLSAFYL DICKDRLYVE APDSLKRRAA QTVMYDCLLS 750
    LVKLVAPLLP HTADEVWAFI PGVEEKSVQL TDMPEGDEQH LSFAAEAESK 800
    WDAFLAIRDE VLKAMEEARR NKVFGNSVDA KLALYPQTEE VAKTLAAMDD 850
    LADLFIVAHV DVHSGSAPAE AVQLEGIAAV VSAADGGKCE RCRVVKPDVG 900
    TRESHASLCV RCADVVEQHY AHVTE 925
    Length:925
    Mass (Da):104,410
    Last modified:May 26, 2009 - v1
    Checksum:i4E716B1C7ED6E053
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008955 Genomic DNA. Translation: BAH44795.1.
    RefSeqiWP_015892079.1. NC_012491.1.
    YP_002773299.1. NC_012491.1.

    Genome annotation databases

    EnsemblBacteriaiBAH44795; BAH44795; BBR47_38180.
    GeneIDi7719311.
    KEGGibbe:BBR47_38180.
    PATRICi21237936. VBIBreBre96763_3785.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008955 Genomic DNA. Translation: BAH44795.1 .
    RefSeqi WP_015892079.1. NC_012491.1.
    YP_002773299.1. NC_012491.1.

    3D structure databases

    ProteinModelPortali C0ZG86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 358681.BBR47_38180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH44795 ; BAH44795 ; BBR47_38180 .
    GeneIDi 7719311.
    KEGGi bbe:BBR47_38180.
    PATRICi 21237936. VBIBreBre96763_3785.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BBRE358681:GHYS-3949-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Brevibacillus brevis strain 47, complete genome."
      Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.
      , Udaka S., Tanikawa S., Fujita N.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 47 / JCM 6285 / NBRC 100599.

    Entry informationi

    Entry nameiSYI_BREBN
    AccessioniPrimary (citable) accession number: C0ZG86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3