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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi491MagnesiumUniRule annotation1
Metal bindingi497MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:BBR47_34250
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
Proteomesi
  • UP000001877 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001857241 – 715Polyribonucleotide nucleotidyltransferaseAdd BLAST715

Interactioni

Protein-protein interaction databases

STRINGi358681.BBR47_34250.

Structurei

3D structure databases

ProteinModelPortaliC0ZF43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini558 – 617KHUniRule annotationAdd BLAST60
Domaini627 – 695S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0ZF43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQQYRTYDF ELAGRKLTLE FGKMAKQAHG SVLVRYGDTA ILSAVTVSKE
60 70 80 90 100
PKPLDFFPLT VNYEERLYAV GKIPGGFIKR EGRPSEKAIL ASRLIDRPVR
110 120 130 140 150
PLFAEGFRND VQIVNTVLSV DQDCSPEIAA MIGTSAALCV SEIPFEGPIA
160 170 180 190 200
GVIVGRIDGE FVINPTVAQA EKSDIHLTVA GTHKAINMVE AAANQVPEAI
210 220 230 240 250
MLEAIMAGHD VIKQLVEFQN MIVSEIGKQK MEVILHEVDP EIDQAVRAYA
260 270 280 290 300
EARLKEAVRI EEKQARYDAI DDIKAETKEH FAAKDAEAYP EQEKMISEVL
310 320 330 340 350
GNIVKDEVRR LITDEKVRPD GRALNEIRPL SSETTILSRT HGSAMFTRGQ
360 370 380 390 400
TQALSVCTLG ALGDVQILDG LGLEESKRFM HHYNFPPYSV GEARPLRPPG
410 420 430 440 450
RREIGHGALG ERAIEPIIPS EVEFPYTIRL VSEVIESNGS TSQASICASV
460 470 480 490 500
LALMDAGVPI KAPVAGIAMG LIMSKDEKSF SILTDIQGME DHLGDMDFKV
510 520 530 540 550
AGTEAGVTAI QMDIKISGIN REILELALEQ ARVGRLHILN HMMSTISEPR
560 570 580 590 600
KELSPYAPKI MTMTINPEKI RDVIGPQGRV INKIIEETGV KIDIEQDGRV
610 620 630 640 650
FIASINHEAN LRAKQIIEDL VREVAVGQVY LGTVKRVEKY GAFIELFAGK
660 670 680 690 700
EGLCHISQLA EERVAKTEDV VAVGDKVQVK VTEIDDQGRV NLSRKAVLKE
710
QAAAEQAAET PTKAE
Length:715
Mass (Da):78,518
Last modified:May 26, 2009 - v1
Checksum:iC4205DC2656751F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH44402.1.
RefSeqiWP_015891706.1. NC_012491.1.

Genome annotation databases

EnsemblBacteriaiBAH44402; BAH44402; BBR47_34250.
KEGGibbe:BBR47_34250.
PATRICi21237166. VBIBreBre96763_3400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH44402.1.
RefSeqiWP_015891706.1. NC_012491.1.

3D structure databases

ProteinModelPortaliC0ZF43.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi358681.BBR47_34250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH44402; BAH44402; BBR47_34250.
KEGGibbe:BBR47_34250.
PATRICi21237166. VBIBreBre96763_3400.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_BREBN
AccessioniPrimary (citable) accession number: C0ZF43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.