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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi140 – 1401MagnesiumUniRule annotation
Metal bindingi143 – 1431MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBBRE358681:GHYS-1977-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:BBR47_18640
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
ProteomesiUP000001877 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 166166Crossover junction endodeoxyribonuclease RuvCPRO_1000195240Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi358681.BBR47_18640.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0ZAN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILGIDPGI AIVGFGVVDK EGSQLRPVQY GSIQTEAGLP VPLRLKQIFE
60 70 80 90 100
AMQSLLETYR PDEMSVEKLF FNKNVTTAFT VGQARGVIIL AAELAGVPVY
110 120 130 140 150
EYTPMQVKQA VTGYGGAEKK QIQEMTKLLL RLKEVPKPDD VADALGIAIT
160
HAQFRAFISI SEGVKK
Length:166
Mass (Da):18,149
Last modified:May 26, 2009 - v1
Checksum:i48EFB99A15138878
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH42841.1.
RefSeqiWP_012685580.1. NC_012491.1.
YP_002771345.1. NC_012491.1.

Genome annotation databases

EnsemblBacteriaiBAH42841; BAH42841; BBR47_18640.
KEGGibbe:BBR47_18640.
PATRICi21234042. VBIBreBre96763_1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA. Translation: BAH42841.1.
RefSeqiWP_012685580.1. NC_012491.1.
YP_002771345.1. NC_012491.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi358681.BBR47_18640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH42841; BAH42841; BBR47_18640.
KEGGibbe:BBR47_18640.
PATRICi21234042. VBIBreBre96763_1855.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciBBRE358681:GHYS-1977-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Brevibacillus brevis strain 47, complete genome."
    Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W., Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S., Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.
    , Udaka S., Tanikawa S., Fujita N.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 47 / JCM 6285 / NBRC 100599.

Entry informationi

Entry nameiRUVC_BREBN
AccessioniPrimary (citable) accession number: C0ZAN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 27, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.