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Protein

Pullulanase

Gene

amyX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Kineticsi

    1. Vmax=27.6 µmol/min/mg enzyme (at pH 5.4)1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei406NucleophileBy similarity1
    Active sitei435Proton donorBy similarity1
    Sitei525Transition state stabilizerBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU29930-MONOMER

    Protein family/group databases

    CAZyiCBM48 Carbohydrate-Binding Module Family 48
    CBM68 Carbohydrate-Binding Module Family 68
    GH13 Glycoside Hydrolase Family 13

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pullulanase (EC:3.2.1.41)
    Alternative name(s):
    Alpha-dextrin endo-1,6-alpha-glucosidase
    Pullulan 6-glucanohydrolase
    Gene namesi
    Name:amyX
    Ordered Locus Names:BSU29930
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003819921 – 718PullulanaseAdd BLAST718

    Proteomic databases

    PaxDbiC0SPA0
    PRIDEiC0SPA0

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100016321

    Structurei

    Secondary structure

    1718
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 14Combined sources8
    Beta strandi17 – 23Combined sources7
    Helixi24 – 26Combined sources3
    Turni27 – 29Combined sources3
    Beta strandi34 – 38Combined sources5
    Beta strandi41 – 52Combined sources12
    Beta strandi54 – 62Combined sources9
    Beta strandi74 – 76Combined sources3
    Beta strandi82 – 84Combined sources3
    Helixi89 – 92Combined sources4
    Helixi94 – 100Combined sources7
    Beta strandi107 – 110Combined sources4
    Beta strandi112 – 120Combined sources9
    Beta strandi125 – 132Combined sources8
    Beta strandi139 – 142Combined sources4
    Helixi147 – 149Combined sources3
    Beta strandi150 – 157Combined sources8
    Beta strandi163 – 170Combined sources8
    Beta strandi173 – 177Combined sources5
    Beta strandi183 – 185Combined sources3
    Helixi187 – 189Combined sources3
    Beta strandi191 – 193Combined sources3
    Helixi212 – 214Combined sources3
    Beta strandi217 – 220Combined sources4
    Helixi222 – 227Combined sources6
    Helixi239 – 243Combined sources5
    Helixi256 – 263Combined sources8
    Beta strandi266 – 271Combined sources6
    Beta strandi274 – 279Combined sources6
    Helixi284 – 286Combined sources3
    Beta strandi293 – 300Combined sources8
    Beta strandi308 – 310Combined sources3
    Helixi311 – 327Combined sources17
    Beta strandi331 – 336Combined sources6
    Helixi344 – 346Combined sources3
    Helixi348 – 352Combined sources5
    Turni354 – 356Combined sources3
    Beta strandi364 – 366Combined sources3
    Beta strandi371 – 373Combined sources3
    Helixi381 – 398Combined sources18
    Beta strandi402 – 405Combined sources4
    Helixi408 – 410Combined sources3
    Helixi413 – 426Combined sources14
    Beta strandi431 – 434Combined sources4
    Helixi445 – 447Combined sources3
    Helixi451 – 456Combined sources6
    Beta strandi461 – 463Combined sources3
    Helixi465 – 472Combined sources8
    Beta strandi475 – 477Combined sources3
    Helixi483 – 485Combined sources3
    Helixi488 – 490Combined sources3
    Helixi491 – 498Combined sources8
    Beta strandi510 – 513Combined sources4
    Helixi514 – 516Combined sources3
    Beta strandi517 – 519Combined sources3
    Beta strandi524 – 527Combined sources4
    Helixi529 – 536Combined sources8
    Helixi542 – 557Combined sources16
    Beta strandi559 – 566Combined sources8
    Helixi569 – 571Combined sources3
    Helixi586 – 589Combined sources4
    Helixi593 – 598Combined sources6
    Helixi600 – 615Combined sources16
    Helixi617 – 620Combined sources4
    Helixi624 – 630Combined sources7
    Beta strandi631 – 636Combined sources6
    Beta strandi638 – 645Combined sources8
    Turni649 – 651Combined sources3
    Beta strandi653 – 662Combined sources10
    Beta strandi664 – 671Combined sources8
    Beta strandi673 – 676Combined sources4
    Beta strandi678 – 683Combined sources6
    Beta strandi686 – 694Combined sources9
    Beta strandi696 – 710Combined sources15

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E8YX-ray2.11A/B1-718[»]
    2E8ZX-ray2.20A/B1-718[»]
    2E9BX-ray2.30A/B1-718[»]
    ProteinModelPortaliC0SPA0
    SMRiC0SPA0
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiC0SPA0

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiENOG4108IUM Bacteria
    COG1523 LUCA
    HOGENOMiHOG000059883
    InParanoidiC0SPA0
    KOiK01200
    OMAiYNRIVEC
    PhylomeDBiC0SPA0

    Family and domain databases

    Gene3Di2.60.40.10, 1 hit
    2.60.40.1180, 1 hit
    InterProiView protein in InterPro
    IPR006047 Glyco_hydro_13_cat_dom
    IPR004193 Glyco_hydro_13_N
    IPR013780 Glyco_hydro_b
    IPR017853 Glycoside_hydrolase_SF
    IPR013783 Ig-like_fold
    IPR014756 Ig_E-set
    IPR011840 PulA_typeI
    PfamiView protein in Pfam
    PF00128 Alpha-amylase, 2 hits
    PF02922 CBM_48, 1 hit
    SMARTiView protein in SMART
    SM00642 Aamy, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit
    SSF81296 SSF81296, 1 hit
    TIGRFAMsiTIGR02104 pulA_typeI, 1 hit

    Sequencei

    Sequence statusi: Complete.

    C0SPA0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSIRRSFEA YVDDMNIITV LIPAEQKEIM TPPFRLETEI TDFPLAVREE
    60 70 80 90 100
    YSLEAKYKYV CVSDHPVTFG KIHCVRASSG HKTDLQIGAV IRTAAFDDEF
    110 120 130 140 150
    YYDGELGAVY TADHTVFKVW APAATSAAVK LSHPNKSGRT FQMTRLEKGV
    160 170 180 190 200
    YAVTVTGDLH GYEYLFCICN NSEWMETVDQ YAKAVTVNGE KGVVLRPDQM
    210 220 230 240 250
    KWTAPLKPFS HPVDAVIYET HLRDFSIHEN SGMINKGKYL ALTETDTQTA
    260 270 280 290 300
    NGSSSGLAYV KELGVTHVEL LPVNDFAGVD EEKPLDAYNW GYNPLHFFAP
    310 320 330 340 350
    EGSYASNPHD PQTRKTELKQ MINTLHQHGL RVILDVVFNH VYKRENSPFE
    360 370 380 390 400
    KTVPGYFFRH DECGMPSNGT GVGNDIASER RMARKFIADC VVYWLEEYNV
    410 420 430 440 450
    DGFRFDLLGI LDIDTVLYMK EKATKAKPGI LLFGEGWDLA TPLPHEQKAA
    460 470 480 490 500
    LANAPRMPGI GFFNDMFRDA VKGNTFHLKA TGFALGNGES AQAVMHGIAG
    510 520 530 540 550
    SSGWKALAPI VPEPSQSINY VESHDNHTFW DKMSFALPQE NDSRKRSRQR
    560 570 580 590 600
    LAAAIILLAQ GVPFIHSGQE FFRTKQGVEN SYQSSDSINQ LDWDRRETFK
    610 620 630 640 650
    EDVHYIRRLI SLRKAHPAFR LRSAADIQRH LECLTLKEHL IAYRLYDLDE
    660 670 680 690 700
    VDEWKDIIVI HHASPDSVEW RLPNDIPYRL LCDPSGFQED PTEIKKTVAV
    710
    NGIGTVILYL ASDLKSFA
    Length:718
    Mass (Da):81,077
    Last modified:May 5, 2009 - v1
    Checksum:i2D23D065C50007E9
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti553A → V in AAC00283 (PubMed:9387221).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF008220 Genomic DNA Translation: AAC00283.1
    AL009126 Genomic DNA Translation: CAB14971.2
    PIRiG69585
    RefSeqiNP_390871.2, NC_000964.3
    WP_003229246.1, NZ_JNCM01000036.1

    Genome annotation databases

    EnsemblBacteriaiCAB14971; CAB14971; BSU29930
    GeneIDi937292
    KEGGibsu:BSU29930
    PATRICifig|224308.179.peg.3251

    Similar proteinsi

    Entry informationi

    Entry nameiPULA_BACSU
    AccessioniPrimary (citable) accession number: C0SPA0
    Secondary accession number(s): O34587, Q795S6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: May 5, 2009
    Last modified: May 23, 2018
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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