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Protein

Pullulanase

Gene

amyX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Kineticsi

    1. Vmax=27.6 µmol/min/mg enzyme (at pH 5.4)1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei406NucleophileBy similarity1
    Active sitei435Proton donorBy similarity1
    Sitei525Transition state stabilizerBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU29930-MONOMER.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    CBM68. Carbohydrate-Binding Module Family 68.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pullulanase (EC:3.2.1.41)
    Alternative name(s):
    Alpha-dextrin endo-1,6-alpha-glucosidase
    Pullulan 6-glucanohydrolase
    Gene namesi
    Name:amyX
    Ordered Locus Names:BSU29930
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003819921 – 718PullulanaseAdd BLAST718

    Proteomic databases

    PaxDbiC0SPA0.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100016321.

    Structurei

    Secondary structure

    1718
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 14Combined sources8
    Beta strandi17 – 23Combined sources7
    Helixi24 – 26Combined sources3
    Turni27 – 29Combined sources3
    Beta strandi34 – 38Combined sources5
    Beta strandi41 – 52Combined sources12
    Beta strandi54 – 62Combined sources9
    Beta strandi74 – 76Combined sources3
    Beta strandi82 – 84Combined sources3
    Helixi89 – 92Combined sources4
    Helixi94 – 100Combined sources7
    Beta strandi107 – 110Combined sources4
    Beta strandi112 – 120Combined sources9
    Beta strandi125 – 132Combined sources8
    Beta strandi139 – 142Combined sources4
    Helixi147 – 149Combined sources3
    Beta strandi150 – 157Combined sources8
    Beta strandi163 – 170Combined sources8
    Beta strandi173 – 177Combined sources5
    Beta strandi183 – 185Combined sources3
    Helixi187 – 189Combined sources3
    Beta strandi191 – 193Combined sources3
    Helixi212 – 214Combined sources3
    Beta strandi217 – 220Combined sources4
    Helixi222 – 227Combined sources6
    Helixi239 – 243Combined sources5
    Helixi256 – 263Combined sources8
    Beta strandi266 – 271Combined sources6
    Beta strandi274 – 279Combined sources6
    Helixi284 – 286Combined sources3
    Beta strandi293 – 300Combined sources8
    Beta strandi308 – 310Combined sources3
    Helixi311 – 327Combined sources17
    Beta strandi331 – 336Combined sources6
    Helixi344 – 346Combined sources3
    Helixi348 – 352Combined sources5
    Turni354 – 356Combined sources3
    Beta strandi364 – 366Combined sources3
    Beta strandi371 – 373Combined sources3
    Helixi381 – 398Combined sources18
    Beta strandi402 – 405Combined sources4
    Helixi408 – 410Combined sources3
    Helixi413 – 426Combined sources14
    Beta strandi431 – 434Combined sources4
    Helixi445 – 447Combined sources3
    Helixi451 – 456Combined sources6
    Beta strandi461 – 463Combined sources3
    Helixi465 – 472Combined sources8
    Beta strandi475 – 477Combined sources3
    Helixi483 – 485Combined sources3
    Helixi488 – 490Combined sources3
    Helixi491 – 498Combined sources8
    Beta strandi510 – 513Combined sources4
    Helixi514 – 516Combined sources3
    Beta strandi517 – 519Combined sources3
    Beta strandi524 – 527Combined sources4
    Helixi529 – 536Combined sources8
    Helixi542 – 557Combined sources16
    Beta strandi559 – 566Combined sources8
    Helixi569 – 571Combined sources3
    Helixi586 – 589Combined sources4
    Helixi593 – 598Combined sources6
    Helixi600 – 615Combined sources16
    Helixi617 – 620Combined sources4
    Helixi624 – 630Combined sources7
    Beta strandi631 – 636Combined sources6
    Beta strandi638 – 645Combined sources8
    Turni649 – 651Combined sources3
    Beta strandi653 – 662Combined sources10
    Beta strandi664 – 671Combined sources8
    Beta strandi673 – 676Combined sources4
    Beta strandi678 – 683Combined sources6
    Beta strandi686 – 694Combined sources9
    Beta strandi696 – 710Combined sources15

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E8YX-ray2.11A/B1-718[»]
    2E8ZX-ray2.20A/B1-718[»]
    2E9BX-ray2.30A/B1-718[»]
    ProteinModelPortaliC0SPA0.
    SMRiC0SPA0.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiC0SPA0.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiENOG4108IUM. Bacteria.
    COG1523. LUCA.
    HOGENOMiHOG000059883.
    InParanoidiC0SPA0.
    KOiK01200.
    OMAiYNRIVEC.
    PhylomeDBiC0SPA0.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR011840. PulA_typeI.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 2 hits.
    PfamiPF00128. Alpha-amylase. 2 hits.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR02104. pulA_typeI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C0SPA0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSIRRSFEA YVDDMNIITV LIPAEQKEIM TPPFRLETEI TDFPLAVREE
    60 70 80 90 100
    YSLEAKYKYV CVSDHPVTFG KIHCVRASSG HKTDLQIGAV IRTAAFDDEF
    110 120 130 140 150
    YYDGELGAVY TADHTVFKVW APAATSAAVK LSHPNKSGRT FQMTRLEKGV
    160 170 180 190 200
    YAVTVTGDLH GYEYLFCICN NSEWMETVDQ YAKAVTVNGE KGVVLRPDQM
    210 220 230 240 250
    KWTAPLKPFS HPVDAVIYET HLRDFSIHEN SGMINKGKYL ALTETDTQTA
    260 270 280 290 300
    NGSSSGLAYV KELGVTHVEL LPVNDFAGVD EEKPLDAYNW GYNPLHFFAP
    310 320 330 340 350
    EGSYASNPHD PQTRKTELKQ MINTLHQHGL RVILDVVFNH VYKRENSPFE
    360 370 380 390 400
    KTVPGYFFRH DECGMPSNGT GVGNDIASER RMARKFIADC VVYWLEEYNV
    410 420 430 440 450
    DGFRFDLLGI LDIDTVLYMK EKATKAKPGI LLFGEGWDLA TPLPHEQKAA
    460 470 480 490 500
    LANAPRMPGI GFFNDMFRDA VKGNTFHLKA TGFALGNGES AQAVMHGIAG
    510 520 530 540 550
    SSGWKALAPI VPEPSQSINY VESHDNHTFW DKMSFALPQE NDSRKRSRQR
    560 570 580 590 600
    LAAAIILLAQ GVPFIHSGQE FFRTKQGVEN SYQSSDSINQ LDWDRRETFK
    610 620 630 640 650
    EDVHYIRRLI SLRKAHPAFR LRSAADIQRH LECLTLKEHL IAYRLYDLDE
    660 670 680 690 700
    VDEWKDIIVI HHASPDSVEW RLPNDIPYRL LCDPSGFQED PTEIKKTVAV
    710
    NGIGTVILYL ASDLKSFA
    Length:718
    Mass (Da):81,077
    Last modified:May 5, 2009 - v1
    Checksum:i2D23D065C50007E9
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti553A → V in AAC00283 (PubMed:9387221).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00283.1.
    AL009126 Genomic DNA. Translation: CAB14971.2.
    PIRiG69585.
    RefSeqiNP_390871.2. NC_000964.3.
    WP_003229246.1. NZ_JNCM01000036.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14971; CAB14971; BSU29930.
    GeneIDi937292.
    KEGGibsu:BSU29930.
    PATRICi18977892. VBIBacSub10457_3134.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00283.1.
    AL009126 Genomic DNA. Translation: CAB14971.2.
    PIRiG69585.
    RefSeqiNP_390871.2. NC_000964.3.
    WP_003229246.1. NZ_JNCM01000036.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E8YX-ray2.11A/B1-718[»]
    2E8ZX-ray2.20A/B1-718[»]
    2E9BX-ray2.30A/B1-718[»]
    ProteinModelPortaliC0SPA0.
    SMRiC0SPA0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100016321.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    CBM68. Carbohydrate-Binding Module Family 68.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbiC0SPA0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB14971; CAB14971; BSU29930.
    GeneIDi937292.
    KEGGibsu:BSU29930.
    PATRICi18977892. VBIBacSub10457_3134.

    Phylogenomic databases

    eggNOGiENOG4108IUM. Bacteria.
    COG1523. LUCA.
    HOGENOMiHOG000059883.
    InParanoidiC0SPA0.
    KOiK01200.
    OMAiYNRIVEC.
    PhylomeDBiC0SPA0.

    Enzyme and pathway databases

    BioCyciBSUB:BSU29930-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiC0SPA0.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR011840. PulA_typeI.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 2 hits.
    PfamiPF00128. Alpha-amylase. 2 hits.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR02104. pulA_typeI. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPULA_BACSU
    AccessioniPrimary (citable) accession number: C0SPA0
    Secondary accession number(s): O34587, Q795S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: May 5, 2009
    Last modified: November 2, 2016
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.