Reviewed, UniProtKB/Swiss-Prot C0SPA0 (PULA_BACSU)

Names and origin

Protein names

Recommended name:
    Pullulanase
    EC=3.2.1.41
Alternative name(s):
    Alpha-dextrin endo-1,6-alpha-glucosidase
    Pullulan 6-glucanohydrolase

Gene names

Name:	amyX
Ordered Locus Names:	BSU29930

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Biophysicochemical properties	Kinetic parameters: Vmax=27.6 µmol/min/mg enzyme (at pH 5.4) pH dependence: Optimum pH is 6.0. Temperature dependence: Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro pullulanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Pullulanase		PRO_0000381992
Sites
	Active site	406	1	Nucleophile By similarity
	Active site	435	1	Proton donor By similarity
	Active site	525	1	By similarity
Experimental info
	Sequence conflict	553	1	A → V in AAC00283. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	C0SPA0-1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 2D23D065C50007E9	FASTA	718	81,077
	10 20 30 40 50 60 MVSIRRSFEA YVDDMNIITV LIPAEQKEIM TPPFRLETEI TDFPLAVREE YSLEAKYKYV 70 80 90 100 110 120 CVSDHPVTFG KIHCVRASSG HKTDLQIGAV IRTAAFDDEF YYDGELGAVY TADHTVFKVW 130 140 150 160 170 180 APAATSAAVK LSHPNKSGRT FQMTRLEKGV YAVTVTGDLH GYEYLFCICN NSEWMETVDQ 190 200 210 220 230 240 YAKAVTVNGE KGVVLRPDQM KWTAPLKPFS HPVDAVIYET HLRDFSIHEN SGMINKGKYL 250 260 270 280 290 300 ALTETDTQTA NGSSSGLAYV KELGVTHVEL LPVNDFAGVD EEKPLDAYNW GYNPLHFFAP 310 320 330 340 350 360 EGSYASNPHD PQTRKTELKQ MINTLHQHGL RVILDVVFNH VYKRENSPFE KTVPGYFFRH 370 380 390 400 410 420 DECGMPSNGT GVGNDIASER RMARKFIADC VVYWLEEYNV DGFRFDLLGI LDIDTVLYMK 430 440 450 460 470 480 EKATKAKPGI LLFGEGWDLA TPLPHEQKAA LANAPRMPGI GFFNDMFRDA VKGNTFHLKA 490 500 510 520 530 540 TGFALGNGES AQAVMHGIAG SSGWKALAPI VPEPSQSINY VESHDNHTFW DKMSFALPQE 550 560 570 580 590 600 NDSRKRSRQR LAAAIILLAQ GVPFIHSGQE FFRTKQGVEN SYQSSDSINQ LDWDRRETFK 610 620 630 640 650 660 EDVHYIRRLI SLRKAHPAFR LRSAADIQRH LECLTLKEHL IAYRLYDLDE VDEWKDIIVI 670 680 690 700 710 HHASPDSVEW RLPNDIPYRL LCDPSGFQED PTEIKKTVAV NGIGTVILYL ASDLKSFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168." Malle D., Itoh T., Hashimoto W., Murata K., Utsumi S., Mikami B. Acta Crystallogr. F 62:381-384(2006) [PubMed: 16582490] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES. Strain: 168.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF008220 Genomic DNA. Translation: AAC00283.1.
AL009126 Genomic DNA. Translation: CAB14971.2.

PIR

G69585.

RefSeq

NP_390871.2.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E8Y	X-ray	2.11	A/B	1-718	[»]
2E8Z	X-ray	2.20	A/B	1-718	[»]
2E9B	X-ray	2.30	A/B	1-718	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Genome annotation databases

GeneID

937292.

GenomeReviews

Gene locus BSU29930 in contig AL009126_GR.

NMPDR

fig|224308.1.peg.2996.

Organism-specific databases

SubtiList

BG12566. amyX. [Micado]

CMR

Search...

Family and domain databases

InterPro

IPR006047. Glyco_hydro_13_cat.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
IPR013783. Ig-like_fold.
IPR011840. PulA_typeI.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02104. pulA_typeI. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PULA_BACSU

Accession

Primary (citable) accession number: C0SPA0
Secondary accession number(s): O34587, Q795S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	May 5, 2009
Last modified:	November 24, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)