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C0SPA0 (PULA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pullulanase
EC=3.2.1.41
Alternative name(s):
Alpha-dextrin endo-1,6-alpha-glucosidase
Pullulan 6-glucanohydrolase
Gene names
Name:amyX
Ordered Locus Names:BSU29930
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Biophysicochemical properties

Kinetic parameters:

Vmax=27.6 µmol/min/mg enzyme (at pH 5.4) Ref.4

pH dependence:

Optimum pH is 6.0.

Temperature dependence:

Optimum temperature is 40 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Pullulanase
PRO_0000381992

Sites

Active site4061Nucleophile By similarity
Active site4351Proton donor By similarity
Site5251Transition state stabilizer By similarity

Experimental info

Sequence conflict5531A → V in AAC00283. Ref.1

Secondary structure

1 ................................................................................................................ 718
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
C0SPA0 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 2D23D065C50007E9

FASTA71881,077
        10         20         30         40         50         60 
MVSIRRSFEA YVDDMNIITV LIPAEQKEIM TPPFRLETEI TDFPLAVREE YSLEAKYKYV 

        70         80         90        100        110        120 
CVSDHPVTFG KIHCVRASSG HKTDLQIGAV IRTAAFDDEF YYDGELGAVY TADHTVFKVW 

       130        140        150        160        170        180 
APAATSAAVK LSHPNKSGRT FQMTRLEKGV YAVTVTGDLH GYEYLFCICN NSEWMETVDQ 

       190        200        210        220        230        240 
YAKAVTVNGE KGVVLRPDQM KWTAPLKPFS HPVDAVIYET HLRDFSIHEN SGMINKGKYL 

       250        260        270        280        290        300 
ALTETDTQTA NGSSSGLAYV KELGVTHVEL LPVNDFAGVD EEKPLDAYNW GYNPLHFFAP 

       310        320        330        340        350        360 
EGSYASNPHD PQTRKTELKQ MINTLHQHGL RVILDVVFNH VYKRENSPFE KTVPGYFFRH 

       370        380        390        400        410        420 
DECGMPSNGT GVGNDIASER RMARKFIADC VVYWLEEYNV DGFRFDLLGI LDIDTVLYMK 

       430        440        450        460        470        480 
EKATKAKPGI LLFGEGWDLA TPLPHEQKAA LANAPRMPGI GFFNDMFRDA VKGNTFHLKA 

       490        500        510        520        530        540 
TGFALGNGES AQAVMHGIAG SSGWKALAPI VPEPSQSINY VESHDNHTFW DKMSFALPQE 

       550        560        570        580        590        600 
NDSRKRSRQR LAAAIILLAQ GVPFIHSGQE FFRTKQGVEN SYQSSDSINQ LDWDRRETFK 

       610        620        630        640        650        660 
EDVHYIRRLI SLRKAHPAFR LRSAADIQRH LECLTLKEHL IAYRLYDLDE VDEWKDIIVI 

       670        680        690        700        710 
HHASPDSVEW RLPNDIPYRL LCDPSGFQED PTEIKKTVAV NGIGTVILYL ASDLKSFA

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168."
Malle D., Itoh T., Hashimoto W., Murata K., Utsumi S., Mikami B.
Acta Crystallogr. F 62:381-384(2006) [PubMed: 16582490] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF008220 Genomic DNA. Translation: AAC00283.1.
AL009126 Genomic DNA. Translation: CAB14971.2.
PIRG69585.
RefSeqNP_390871.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E8YX-ray2.11A/B1-718[»]
2E8ZX-ray2.20A/B1-718[»]
2E9BX-ray2.30A/B1-718[»]
ProteinModelPortalC0SPA0.
SMRC0SPA0. Positions 1-712.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000000438; EBBACP00000000438; EBBACG00000000436.
GeneID937292.
GenomeReviewsGene locus BSU29930 in contig AL009126_GR.
KEGGbsu:BSU29930.
NMPDRfig|224308.1.peg.2996.
PATRIC18977892. VBIBacSub10457_3134.

Organism-specific databases

GenoListBSU29930. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000031950.
HOGENOMHBG697609.
PhylomeDBC0SPA0.
ProtClustDBCLSK873560.

Enzyme and pathway databases

BioCycBSUB:BSU29930-MONOMER.

Family and domain databases

InterProIPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR011840. PulA_typeI.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KOK01200.
PANTHERPTHR10357. Alpha_amylase. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02922. CBM_48. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMsTIGR02104. PulA_typeI. 1 hit.
ProtoNetSearch...

Entry information

Entry namePULA_BACSU
AccessionPrimary (citable) accession number: C0SPA0
Secondary accession number(s): O34587, Q795S6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: May 5, 2009
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents